Acta Biologica Hungarica

, Volume 64, Issue 2, pp 161–168 | Cite as

Temporospatial Expression of Dmrt1 in Chicken Urogenital System (Gallus Gallus) using Whole Mount In Situ Hybridization

  • Y. Yang
  • Ping Gong
  • Yan P. Feng
  • S. J. Li
  • Xiu L. Peng
  • Z. P. Ran
  • Y. G. Qian
  • Y. Z. GongEmail author


Doublesex and mab-3-related transcription factor 1 (Dmrt1) is a Z-linked gene that putatively determines the phenotype of gonads in birds. The sex differential expression of Dmrt1 was examined using wholemount in situ hybridization (WISH) in the urogenital systems during embryogenesis. The results revealed that Dmrt1 showed dimorphic expression in chicken gonads, which increased from day 6.5 to day 10.5. The expression of Dmrt1 in male (ZZ) gonads was not twice as much as in female (ZW) gonads, suggesting the existence of other regulatory mechanisms in addition to Z chromosome dosage effect.


Dmrt1 gene whole-mount in situ hybridization chicken embryos testes ovaries 


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© Akadémiai Kiadó, Budapest 2013

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Y. Yang
    • 1
    • 2
  • Ping Gong
    • 2
  • Yan P. Feng
    • 1
  • S. J. Li
    • 1
  • Xiu L. Peng
    • 1
  • Z. P. Ran
    • 2
  • Y. G. Qian
    • 2
  • Y. Z. Gong
    • 1
    Email author
  1. 1.Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of EducationHuazhong Agricultural UniversityWuhan, HubeiP. R. China
  2. 2.Wuhan Institute of Animal Husbandry and Veterinary ScienceWuhan, Hubei

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