Whole Body Static Magnetic Field Exposure Increases Thermal Nociceptive Threshold in the Snail, helix Pomatia
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We investigated the effect of homogeneous and inhomogeneous static magnetic field (SMF) exposure on the thermal nociceptive threshold of snail in the hot plate test (43 °C). Both homogeneous (hSMF) and inhomogeneous (iSMF) SMF increased the thermo-nociceptive threshold: 40.2%, 29.2%, or 41.7% after an exposure of 20, 30, or 40 min hSMF by p<0.001, p<0.0001, or p<0.001, and 32.7% or 46.2% after an exposure of 20 or 40 min iSMF by p<0.05 or p<0.0001. These results suggest that SMF has an antinociceptive effect in snail. On the other hand, naloxone as an atypical opioid antagonist in an amount of 1 μg/g was found to significantly decrease the thermo-nociceptive threshold (41.9% by p<0.002), which could be antagonized by hSMF exposure implying that hSMF exerts its antinociceptive effect partly via opioid receptors.
KeywordsStatic magnetic field (SMF) nociception naloxone analgesia opioid receptors-snail
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The authors are grateful to Prof. Zs. Fürst and Dr. M. Al-Khrasani for their kind contribution to the measurements.
- 1.Achaval, M., Penha, M. A. P., Swarowsky, A., Rigon, P., Xavier, L. L., Viola, G. G., Zancan, D. M. (2005) The terrestrial gastropoda Megalobulimus abbreviates as a useful model for nociceptive experiments. Effects of morphine and naloxone on thermal avoidance behavior. Brazilian J. Med Biol. Res. 38, 73–80.Google Scholar
- 3.Burrowes, W. R., Assanah, P., Stefano, G. B. (1983) Behavioral effects of opiates on the land snail Helix aspersa. Life Sci. 33S1, 381–384.Google Scholar
- 4.Chase, R. (2002) Behavior and its neural control in gastropod mollusks. Oxford Univ. Press.Google Scholar
- 5.Crozier, S., Trakic, A., Wang, H., Liu, F. (2007) Numerical study of currents in workers induced by body-motion around high-ultrahigh field magnets. J. Magn. Res. Imaging 26, 1261–1277.Google Scholar
- 6.Del Seppia, C., Ghionea, S., Luschib, P., Ossenkopp, K. P., Choleris, E., Kavaliers, M. (2007) Pain perception and electromagnetic fields. Neurosc. Biobehav. R. 31, 619–642.Google Scholar
- 7.Elekes, K., Stefano, G. B., Carpenter, D. O. (1993) Enkephalin-like immunoreactive neurons in the central nervous system of gastropods (Helix pomatia, Lymnaea stagnalis, Aplysia californica): a comparative immunocytochemical study. Cell Tiss. Res. 272, 329–341.Google Scholar
- 9.Hernadi, L., Vehovszky, A., Gyori, J., Hiripi, L. (2008) Neuronal background of activation of aesti-vated snails. With special attention to the monoaminergic system: a biochemical, physiological, and neuroanatomical study. Cell Tiss. Res. 331, 539–553.Google Scholar
- 11.Jokela, K., Saunders, R. D. (2011) Physiologic and dosimetric considerations for limiting electric fields induced in the body by movement in a static magnetic field. Health Rhys. 100(6), 641–653.Google Scholar
- 20.Laszlo, J. F. (2011) External static magnetic field as a device for self-motion perception: A pathophysiological rodent model and its consequences. Horizons in Neuroscience Research. Volume 5 (Eds. A Costa, E Villalba) Nova Science Publishers, Hauppauge, NY, USA, pp. 106–124.Google Scholar
- 21.Laszlo, J., Reiczigel, J., Szekely, L., Gasparics, A., Bogar, I., Bors, L., Racz, B., Gyires, K. (2007) Optimization of static magnetic field parameters improves analgesic effect in mice. Bioelectromagnetics 25, 615–627.Google Scholar
- 22.Laszlo, J., Timar, J., Gyarmati, Zs., Fiirst, Zs., Gyires, K. (2009) Pain-inhibiting inhomogeneous static magnetic field fails to influence locomotor activity and anxiety behaviour in mice: no interference between magnetic field- and morphine-treatment. Brain Res. Bull. 79, 316–321.PubMedGoogle Scholar
- 24.Miller-Perez, C., Sanzes-Islas, E., Pellicer, F., Rodriguez-Manzo, G., Cruz, S. L., Leon-Olea, M. (2008) Role of nociceptin/orphanin FQ and the pseudopeptide [Phe1 <P(CH2NH) Gly2]-nociceptin (1-13)-NH2 and their interaction with classic opioids in the modulation of thermonociception in the land snail Helix aspersa. E. J. Pharmacol. 581, 77–85.Google Scholar
- 25.Nikolic, L., Kartelija, G., Nedeljkovic, M. (2008) Effect of static magnetic fields on bioelectric properties of the Br and Nl neurons of snail Helix pomatia. Comp. Biochem. Physiol. A 151, 657–663.Google Scholar
- 26.Norekian, T. P., Sakharov, D. A. (1991) Mechanoreception in the pteropod mollusk Clione limachina: tactile inputs are blocked by opiate anatagonist. Sensory Systemy 5, 5–11.Google Scholar
- 27.Pivovarov, A. S. (1993) Differently directed modulation of cholinoreceptor plasticity of RPa3 and LPa3 neurons by opiate mu and kappa agonists in the common snail. Zh. Vyssh. Nerv. Deyat. 43, 826–836.Google Scholar
- 28.Prato, F. S., Kavaliers, M., Carson, J. L. (1996) Behavioural responses to magnetic fields by land snails are dependent on both magnetic field direction and light. Proc. Roy Soc. LB 263, 1437–1442.Google Scholar
- 30.Rosen, A. D. (1992) Magnetic field influence on acetylcholine release at the neuromuscular junction. Am. J. Physiol. C 262, 1418–1422.Google Scholar
- 33.Rosen, A. D. (2003) Mechanism of action of moderate intensity static magnetic fields on biological systems. Cell Biochem. Biophysics 39, 163–173.Google Scholar
- 37.Satow, Y., Satake, H., Matsunami, K. (1990) Effect of long exposure to large static magnetic field on the recovery process of bullfrog sciatic nerve activity. Proc. Jpn. Acad. 66, 151–155.Google Scholar
- 41.Stefano, G. B., Vadasz, I., Hiripi, L. (1980) Methionine enkephalin inhibits the bursting activity of the Br type neuron in Helix pomatia L. Experientia 15, 666–667.Google Scholar
- 42.Thomas, A. W., Kavaliers, M., Prato, F. S., Ossenkopp, K. P. (1997) Pulsed magnetic field induced analgesia in the land snail Cepaea nemoralis, and the effect of u, S, and K opioid receptor agonist/ antagonist. Peptides 18, 703–709.Google Scholar
- 43.Waldhoer, M, Bartlett, S. E., Whistler, J. L. (2004) Opioid receptors. Ann. Rev. Biochem. 73, 953–990.Google Scholar
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