Repeated Simultaneous Cortical Electrophysiological and Behavioral Recording in Rats Exposed to Manganese-Containing Nanoparticles
Male Wistar rats wearing chronically implanted cortical electrodes were exposed to Mn-containing nano-particles via the airways for 8 weeks following a 2-week pre-exposure period. The rats’ cortical electrical activity and open field motility was recorded simultaneously, in weekly repetitions. It was supposed that this technique can provide better insight in the development of Mn-induced CNS damage. Decreased motility (less distance covered, longer periods of immobility) and increased total power of cortical electrical activity developed in parallel in the first 4–5 weeks of treatment but showed little change afterwards. Both the behavioral and the electrophysiological effect were in fair correlation with the rats’ internal Mn exposure determined from brain samples. The results confirmed the non-linear dose- and time-dependence of Mn effects suggested by previous studies. Repeated simultaneous behavioral and electrophysiological recording during a longer treatment with neurotoxic metals (or other xenobiotics) seems to be a promising method.
KeywordsManganese rat cortical electrical activity open field motility chronic recording
The authors are thankful to Mr. József Koszta and Ms. Edit Pálinkás at the laboratory of the MOL Hungarian Oil and Gas Company for the manganese level determinations.
- 6.Couper, J. (1837) On the effects of black oxide of manganese when inhaled into the lungs. Br. Ann Med. Pharm. Vit. Stat. Gen. Sci. 1, 41–42.Google Scholar
- 11.ICRP (1994) Human respiratory tract model for radiological protection. A report of a task group of the ICRP. Annals of the International Commission on Radiation Protection, ICRP Publication 66, Pergamon Press, OxfordGoogle Scholar
- 12.Mate, Zs., Szabo, A., Oszlanczi, G., Papp, A. (2011) Modelling per os Mn exposure and examining its functional nervous system effects in rats. Egeszsegtudomdny 55, 71–81 (in Hungarian).Google Scholar
- 14.Normandin, L., Beaupre, L. A., Salehi, F., St-Pierre, A., Kennedy, G., Mergler, D., Butterworth, R. F., Philippe, S., Zayed, J. (2004) Manganese distribution in the brain and neurobehavioral changes following inhalation exposure of rats to three chemical forms of manganese. Neurotoxicology 25, 411–433.CrossRefGoogle Scholar
- 21.Takacs, Sz., Papp, A. (2010) Effects of antiepileptics and an anesthetic on basal cortical activity and spontaneous motility in an epilepsy-prone rat strain. Acta Physiol. Hung. 97, 480–181.Google Scholar
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.