Male Wistar rats wearing chronically implanted cortical electrodes were exposed to Mn-containing nano-particles via the airways for 8 weeks following a 2-week pre-exposure period. The rats’ cortical electrical activity and open field motility was recorded simultaneously, in weekly repetitions. It was supposed that this technique can provide better insight in the development of Mn-induced CNS damage. Decreased motility (less distance covered, longer periods of immobility) and increased total power of cortical electrical activity developed in parallel in the first 4–5 weeks of treatment but showed little change afterwards. Both the behavioral and the electrophysiological effect were in fair correlation with the rats’ internal Mn exposure determined from brain samples. The results confirmed the non-linear dose- and time-dependence of Mn effects suggested by previous studies. Repeated simultaneous behavioral and electrophysiological recording during a longer treatment with neurotoxic metals (or other xenobiotics) seems to be a promising method.
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The authors are thankful to Mr. József Koszta and Ms. Edit Pálinkás at the laboratory of the MOL Hungarian Oil and Gas Company for the manganese level determinations.
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Takács, S., Szabó, A., Oszlánczi, G. et al. Repeated Simultaneous Cortical Electrophysiological and Behavioral Recording in Rats Exposed to Manganese-Containing Nanoparticles. BIOLOGIA FUTURA 63, 426–440 (2012). https://doi.org/10.1556/ABiol.63.2012.4.2
- cortical electrical activity
- open field motility
- chronic recording