Acta Biologica Hungarica

, Volume 63, Issue 1, pp 113–127 | Cite as

In Vitro Growth and Organogenesis of Prosopis Farcta Plantlets (Fabaceae, Mimosoideae) in Culture Medium Supplemented with Various Concentrations of Ca++ and Na+

  • S. Stambouli
  • S. Bouzid
  • P. Dutuit
  • Fethia Harzallah-SkhiriEmail author


The objective of this study was to vary the mineral composition of the culture medium of Prosopis farcta seedlings per addition of Na+ and Ca++ ions with the aim to identify the culture media which support the growth and/or the expression of the in vitro plant organogenesis. The Na+ and Ca++ ions were added in the culture medium in various concentrations by taking the Gamborg medium, in which macroelements were diluted 10 times, as the basic one. After two months of culture, parameters relating to the vegetative development of plant seedlings and to the various expressions of organogenesis were measured. Weak concentrations in sodium and calcium ions as well as a weak concentration in Ca++ (0.1 mM) with 50 mM in Na+ support the best vegetative development of the plantlets. The most important percentage of plant seedlings presenting a bud initiation was obtained on a medium containing 0.1 mM of Na+ and 50 mM of Ca++. Our study defined several media likely to support in vitro development of Prosopis farcta plantlets allowing the selection of salt tolerant plants or cellular lines. Some other media were chosen for improving micropropagation of the species without adding growth substances.


Prosopis farcta sodium calcium in vitro growth organogenesis 


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© Akadémiai Kiadó, Budapest 2012

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • S. Stambouli
    • 1
  • S. Bouzid
    • 1
  • P. Dutuit
    • 2
  • Fethia Harzallah-Skhiri
    • 3
    Email author
  1. 1.Morphogenesis and Plant Biotechnology Research Unit, Faculty of Sciences of Tunis University, Tunis El ManarUniversity CampusTunisTunisia
  2. 2.Ecotechnologie Laboratory, Faculty of Pharmacy of Châtenay-MalabryParis-Sud XI UniversityChâtenay-Malabry, Hauts-de-SeineFrance
  3. 3.Higher Institute of Biotechnology of MonastirAgrobiodiversity Research Unit, University of Sousse, Higher Institute of Agronomy of Chott-Meriem, Sousse/University of MonastirMonastirTunisia

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