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Acta Biologica Hungarica

, Volume 63, Issue 1, pp 113–127 | Cite as

In Vitro Growth and Organogenesis of Prosopis Farcta Plantlets (Fabaceae, Mimosoideae) in Culture Medium Supplemented with Various Concentrations of Ca++ and Na+

  • S. Stambouli
  • S. Bouzid
  • P. Dutuit
  • Fethia Harzallah-SkhiriEmail author
Article

Abstract

The objective of this study was to vary the mineral composition of the culture medium of Prosopis farcta seedlings per addition of Na+ and Ca++ ions with the aim to identify the culture media which support the growth and/or the expression of the in vitro plant organogenesis. The Na+ and Ca++ ions were added in the culture medium in various concentrations by taking the Gamborg medium, in which macroelements were diluted 10 times, as the basic one. After two months of culture, parameters relating to the vegetative development of plant seedlings and to the various expressions of organogenesis were measured. Weak concentrations in sodium and calcium ions as well as a weak concentration in Ca++ (0.1 mM) with 50 mM in Na+ support the best vegetative development of the plantlets. The most important percentage of plant seedlings presenting a bud initiation was obtained on a medium containing 0.1 mM of Na+ and 50 mM of Ca++. Our study defined several media likely to support in vitro development of Prosopis farcta plantlets allowing the selection of salt tolerant plants or cellular lines. Some other media were chosen for improving micropropagation of the species without adding growth substances.

Keywords

Prosopis farcta sodium calcium in vitro growth organogenesis 

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References

  1. 1.
    Awada, S., Campbell, W. F., Dudley, L. M., Jurinak, J. J., Khan, M. A. (1995) Interactive effects of sodium-chloride, sodium-sulfate, calcium-sulfate, calcium-chloride on snapbean growth, photosynthesis, ion uptake. J. Plant Nutr. 18, 889–900.CrossRefGoogle Scholar
  2. 2.
    Benrebiha, F., Pourrat, Y., Dutuit, R,. (1992) Induction de la callogenèse chez l’Atriplex halimus sur des milieux de culture dépourvus d’hormones de croissance. Rôle des éléments minéraux. B. Soc. Bot. Fr. 139, 219–222.Google Scholar
  3. 3.
    Buendía-González, L., Orozco-Villafuerte, J., Cruz-Sosa, F., Chávez, Á., Vernon-Carter, E. J. (2007) Clonal propagation of mesquite tree (Prosopis laevigata Humb. & Bonpl. ex Willd. M. C. Johnston) via cotyledonary nodes. In Vitro Cell. Dev.-Pl. 43, 260–266.CrossRefGoogle Scholar
  4. 4.
    Burkart, A. (1976) A monography of the genus Prosopis (Leguminosae Subfam. Mimosoideae). J. Arnold Arboretum 57, 219–249, 450–525.Google Scholar
  5. 5.
    Caro, L. A., Polci, P. A., Lindstrom, L. I., Echenique, C. V., Hernandez, L. F. (2002) Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plants. Biocell 26, 25–33.PubMedGoogle Scholar
  6. 6.
    Chaieb, M. (1992) Biologie et comportement écologique du Prosopis stephaniana (M.B.) Kunth. à la limite Ouest de son aire de répartition (cas du site de Gabès). Forêt méditerranéenne 13, 85–90.Google Scholar
  7. 7.
    Cottin, R. (1988) La taxonomie numérique, application aux agrumes. Fruits 43, 721–733.Google Scholar
  8. 8.
    Cramer, G. R., Epstein, E., Läuchli, A. (1990) Effects of sodium, potassium and calcium on salt-stressed barley. 1 Growth analysis. Plant Physiol. 80, 83–88.CrossRefGoogle Scholar
  9. 9.
    Cramer, G. R. (2002) Sodium-calcium interactions under salinity stress. In: Läuchli, M., Lüttge, U. (eds) Salinity-Environment-Plants-Molecules. Kluwer Academic Publishers, London, pp. 205–228.Google Scholar
  10. 10.
    Crisci, J. V., Lopez Armengol, M. F. (1983) Introduccion a la teoria y practica de la taxonomia numerica. Monografia nro. 26, Serie de Biologia, Programa de Monografias Cientificas. Organizacion de los Estados Americanos (ed.), Washington, D.C.Google Scholar
  11. 11.
    F.A.O. (1985) The current state of knowledge on Prosopis tamarugo. Habit, M. A. (ed.), Tarapaca University, National Forestry Corporation, Arica, Chile.Google Scholar
  12. 12.
    Felker, P., Cannell, G. H., Clark, P. R. (1981) Variation in growth among 13 Prosopis (Mesquite) species. Exp. Agr. 17, 209–218.CrossRefGoogle Scholar
  13. 13.
    Felker, P., Clark, P. R., Laag, A. E., Pratt, P. F. (1981) Salinity tolerance of the tree legumes: Mesquite (Prosopis glandulosa var. torreyana, P. velutina, and P. articulata) Algarrobo (P. Chilensis), kiawe (P. pallida) and Tamarugo (P. tamarugo) grown in sand culture on nitrogen-free media. Plant and Soil 61, 311–317.CrossRefGoogle Scholar
  14. 14.
    Flowers, T. J., Troke, P. F., Yeo, A. R. (1977) The mechanism of salt tolerance in halophytes. Plant Physiol. 28, 89–121.CrossRefGoogle Scholar
  15. 15.
    Gamborg, O. (1970) The effects of amino acids and ammonium on the growth plant cells in suspension culture. Plant Physiol. 45, 372–375.CrossRefGoogle Scholar
  16. 16.
    Gautheret, R. J. (1959) La culture des tissus végétaux. Techniques et réalisations. Masson, Paris.Google Scholar
  17. 17.
    Harzallah-Skhiri, F. (2003) Le Prosopis en Tunisie: diversité biologique aptitudes, morphogénétiques et adaptations aux conditions édapho-climatiques, Thèse de Doctorat d’Etat, Faculté des Sciences de Tunis, Tunisie.Google Scholar
  18. 18.
    Harzallah-Skhiri, F., Ben Ouada, H., Bouzid, S., Dutuit, P. (2004) Diversité morphologique des populations à base de Prosopis, identification et évaluation de ces ressources génétiques. In: Ferchichi, A. (comp.) Réhabilitation des pâturages et des parcours en milieux méditerranéens. Réunion du Sous-Réseau Ressources Fourragères Méditerranéennes du Réseau Coopératif Interrégional FAO-CIHEAM de Recherche et Développement sur les Pâturages et les Cultures Fourragères, 2002/10/29-2002/11/01, Djerba (Tunisie).Google Scholar
  19. 19.
    Hu, Y., Schmidhalter, U. (2005) Drought and salinity: A comparison of their effects on mineral nutrition of plants. J. Plant Nutr. Soil Sci. 168, 541–549.CrossRefGoogle Scholar
  20. 20.
    Kumar, S., Singh, N. (2009) Micropropagation of Prosopis Cineraria (L.) Druce. A Multipurpose Desert Tree. Researcher 1, 3.Google Scholar
  21. 21.
    Marschner, H. (1995) Mineral Nutrition of Higher Plants. 2nd ed., Academic Press Inc., San Diego.Google Scholar
  22. 22.
    Molina Cano, J. L. (1977) Introduction a la taxonomia numerica. Monografias de la Escuela Tecnica superior de Ingenieros Agronomos de Madrid 80.Google Scholar
  23. 23.
    Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15, 473–497.CrossRefGoogle Scholar
  24. 24.
    Mwangi, E., Swallow, B. (2005) Invasion of Prosopis and local livelihoods: Case study from the lake Baringo area of Kenya. ICRAF Working Paper — no. 3, Nairobi, World Agroforestry Centre.Google Scholar
  25. 25.
    Pasiecznik, N. M., Felker, P., Harris, P. J. C., Harsh, L. N., Cruz, G., Tewari, J. C., Cadoret, K., Maldonado, L. J. (2001) The Prosopis juliflora — Prosopis pallida Complex: A Monograph. HDRA, Coventry, UK. https://doi.org/hdra.org.uk/international_programme/ip_publications.htm.Google Scholar
  26. 26.
    Pottier-Alapetite, G. (1979) Flore de la Tunisie Angiospermes-Dicotylédones, Apétales-Dialypétales. Publications Scientifiques tunisiennes 293.Google Scholar
  27. 27.
    Pourrat, Y., Dutuit, P. (1993) Effects of the sodium and calcium concentration on the in vitro growth of Atriplex halimus L. plantlets. J. Plant Nutr. 16, 1417–1429.CrossRefGoogle Scholar
  28. 28.
    Serrato-Valenti, G., Melone, L., Orsi, O., Riveros, F. (1992) Anatomical changes in Prosopis cineraria (L.) Druce seedlings growing at different levels of NaCl salinity. Ann. Bot. London 70, 399–404.CrossRefGoogle Scholar
  29. 29.
    Zaafouri, M. S., Chaieb, M. (1999) Arbres et arbustes de la Tunisie méridionale menacés de disparition (= Threatened trees and shrubs of the Southern Tunisia). Acta Bot. Gallica 146, 361–373.CrossRefGoogle Scholar

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© Akadémiai Kiadó, Budapest 2012

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • S. Stambouli
    • 1
  • S. Bouzid
    • 1
  • P. Dutuit
    • 2
  • Fethia Harzallah-Skhiri
    • 3
    Email author
  1. 1.Morphogenesis and Plant Biotechnology Research Unit, Faculty of Sciences of Tunis University, Tunis El ManarUniversity CampusTunisTunisia
  2. 2.Ecotechnologie Laboratory, Faculty of Pharmacy of Châtenay-MalabryParis-Sud XI UniversityChâtenay-Malabry, Hauts-de-SeineFrance
  3. 3.Higher Institute of Biotechnology of MonastirAgrobiodiversity Research Unit, University of Sousse, Higher Institute of Agronomy of Chott-Meriem, Sousse/University of MonastirMonastirTunisia

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