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Acta Biologica Hungarica

, Volume 63, Issue 1, pp 52–66 | Cite as

Ultrastructural Aspects and Programmed Cell Death in the Tapetal Cells of Lathyrus Undulatus Boiss

  • Filiz VardarEmail author
  • Meral Ünal
Article

Abstract

Programmed cell death (PCD) in the tapetum of Lathyrus undulatus L. was analyzed based on light, fluorescence and electron microscopy to characterize its spatial and temporal occurrence. Development and processes of PCD in secretory tapetal cells of Lathyrus undulatus L. were correlated with the sporog-enous cells and pollen grains. At early stages of development the tapetal cells appeared similar to pollen mother cells, structurally. Concurrent with meiosis, tapetum expanded both tangentially and radially as vacuoles increased in size. Tapetal cells most fully developed at young microspore stage. However, tapetum underwent substantial changes in cell organization including nucleus morphology monitored by DAPI. The TUNEL staining confirmed the occurrence of intra-nucleosomal DNA cleavage. In addition to nuclear degeneration which is the first hallmark of PCD other diagnostic features were observed at vacuolated microspore stage intensely; such as chromatin condensation at the periphery of the nucleus, nuclear membrane degeneration, chromatin release to the cytoplasm, vacuole collapse according to tono-plast rupture, shrinkage of the cytoplasm, the increase and enlargement of the endoplasmic reticulum cisternae and disruption of the plasma membrane. After vacuole collapse due to possible release of hydro-lytic enzymes the cell components degraded. Tapetal cells completely degenerated at bicellular pollen stage.

Keywords

Lathyrus undulatus Boiss. programmed cell death tapetum TUNEL vacuole collapse 

Abbreviations

DAPI

4’,6-diamidino-2-phenylindole

ER

Endoplasmic reticulum

nDNA

nuclear deoxyribonucleic acid

PCD

Programmed Cell Death

TEM

Transmission electron microscopy

TUNEL

Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labelling

VPE

Vacuolar processing enzyme

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© Akadémiai Kiadó, Budapest 2012

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Science and Art Faculty, Department of BiologyMarmara UniversityGöztepe, İstanbulTurkey

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