Advertisement

Acta Biologica Hungarica

, Volume 63, Issue 1, pp 151–161 | Cite as

Phylogeny, Coat Protein Genetic Variability, and Transmission Via Seeds of Hosta Virus X

  • Sun H. Choi
  • Min H. Park
  • K. H. RyuEmail author
Article

Abstract

The complete genome of Hosta Virus X (HVX), which is thought to be a distinct species of Potexvirus, was sequenced. Nucleotide sequences of HVX were compared with those of other members of the genus Potexvirus and phylogenetic tree was constructed. The range of identities of viral replicase open reading frame 1 (ORF1) between HVX and other potexviruses were 43.1%–55.1% and 35.9%–46.6% at the nucleotide and amino acid levels, respectively. Phylogenetic analysis was performed according to the amino acid sequence of the replicase to determine the position of HVX in the genus Potexvirus. Results from the phylogenetic analysis demonstrated that HVX was in the same group as Cassava common mosaic virus (CsCMV), Plantago asiatica mosaic virus (PlAMV), Tulip virus X (TVX), and Hydrangea ring spot virus (HdRSV). In particular, coat protein (CP) sequences among viruses from different Hosta cultivars were revealed to be less variable than those from different isolates of Potato virus X (PVX), a Potexvirus type species. In the present study, HVX was transmissible by seeds of the Hosta “Blue Cadet” cultivar. Moreover, HVX was detected in the embryo but not in the seed coat or endosperm of the seed.

Keywords

Hosta longipes HVX Potexvirus phylogeny seed transmission 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Calvert, L. A., Cuervo, M. I., Ospina, M. D., Fauquet, C. M., Ramirez, B. (1996) Characterization of cassava common mosaic virus and a defective RNA species. J. Gen. Virol. 77, 525–530.CrossRefGoogle Scholar
  2. 2.
    Chen, J., Zheng, H. Y., Chen, J. R, Adams, M. J. (2002) Characterisation of a potyvirus and a potex-virus from Chinese scallion. Arch. Virol. 147, 683–693.CrossRefGoogle Scholar
  3. 3.
    Chen, J., Shi, Y. H., Adams, M. J., Chen, J. P. (2005) The complete sequence of the genomic RNA of an isolate of Lily virus X (genus Potexvirus). Arch. Virol. 150, 825–832.CrossRefGoogle Scholar
  4. 4.
    Choi, S. H., Ryu, K. H. (2008) Determination of complete genome sequence of Korean isolate of Potato virus X. Plant Pathol. J. 24, 361–364.CrossRefGoogle Scholar
  5. 5.
    Cote, F., Pare, C., Majeau, N., Bolduc, M., Leblanc, E., Bergeron, M. G., Bernardy, M. G., Leclerc, D. (2007) Nucleotide sequence and phylogenetic analysis of a new potexvirus: Malva Mosaic Virus. Infection, Genetics and Evolution 8, 83–93.CrossRefGoogle Scholar
  6. 6.
    Cotillon, A. C., Girard, M., Ducouret, S. (2002) Complete nucleotide sequence of the genomic RNA of a French isolate of Pepino mosaic virus (PepMV). Arch. Virol. 147, 2231–2238.CrossRefGoogle Scholar
  7. 7.
    Currier, S., Lockhart, B. E. L. (1996) Characterization of a potexvirus infecting Hosta spp. Plant Dis. 80, 1040–1043.CrossRefGoogle Scholar
  8. 8.
    Dizadji, A., Joohi-Habibi, M., Izadpanah, K., Dietrich, C., Mossahebi, G. H., Winter, S. (2008) Characterisation of lettuce virus X, a new potexvirus infecting lettuce in Iran. Arch. Virol. 153, 1867–1875.CrossRefGoogle Scholar
  9. 9.
    Dolja, V. V., Boyko, V. P., Agranovsky, A. A., Koomin, E. V. (1991) Phylogeny of the capsid proteins of rod-shaped and filamentous RNA plant viruses: two families with distinct patterns of sequence and probably structure conservation. Virology 184, 79–86.CrossRefGoogle Scholar
  10. 10.
    Fajolu, O. L., Wen, R. H., Windham, A. S., Windham, M. T., Moulton, J. K., Hajimorad, M. R. (2009) Genetic variability and phylogenetic analysis of hosta virus X. Arch. Virol. 154, 1909–1916.CrossRefGoogle Scholar
  11. 11.
    Fuji, S., Shinoda, K., Ikeda, M., Furuya, H., Naito, H., Fukumoto, F. (2005) Complete nucleotide sequence of the new potexvirus “Alstroemeria virus X”. Arch. Virol. 150, 2377–2385.+CrossRefGoogle Scholar
  12. 12.
    Fuji, S., Shinoda, K., Furuya, H., Naito, H., Fukumoto, F. (2006) Complete nucleotide sequence of Nerine virus X (NVX-J) isolated from the African lily plant (Agapanthus camanulatus) in Japan. Arch. Virol. 151, 205–208.CrossRefGoogle Scholar
  13. 13.
    Hammond, J., Reinsel, M. D., Maroon-Lango, C. J. (2006) Identification and full sequence of an isolate of Alternanthera mosaic potexvirus infecting Phlox stolonifera. Arch. Virol. 151, 477–493.CrossRefGoogle Scholar
  14. 14.
    Hughes, P. L., Lawrence, J. E., Scott, S. W. (2005) The complete nucleotide sequence of Hydrangea ringspot virus. Arch. Virol. 150, 2339–2345.CrossRefGoogle Scholar
  15. 15.
    Jelkmann, W., Maiss, E., Martin, R. R. (1992) The nucleotide sequence and genome organization of strawberry mild yellow edge-associated potexvirus. J. Gen. Virol. 73, 475–479.CrossRefGoogle Scholar
  16. 16.
    Kanyuka, K. V., Vishnichenko, V. K., Levay, K. E., Kondrikov, D. Yu., Ryabov, E. V., Zavriev, S. K. (1992) Nucleotide sequence of Shallot Virus X RNA reveals a 5′-proximal cistron closely related to those of potexviruses and a unique arrangement of the 3′-proximal cistrons. J. Gen. Virol. 73, 2553–2560.CrossRefGoogle Scholar
  17. 17.
    Lockhart, B. E. L. (2002) Differential response of Hosta cultivars to infection by Hosta Virus X Potexvirus — A basis for practical disease management. ActaHort. 586, 69–72.Google Scholar
  18. 18.
    Marchler-Bauer, A., Anderson, J. B., Derbyshire, M. K., DeWeese-Scott, C., Gonzales, N. R., Gwadz, M., Hao, L., He, S., Hurwitz, D. I., Jackson, J. D., Ke, Z., Krylov, D., Lanczycki, C. J., Liebert, C. A., Liu, C., Lu, F., Lu, S., Marchler, G. H., Mullokandov, M., Song, J. S., Thanki, N., Yamashita, R. A., Yin, J. J., Zhang, D., Bryant, S. H. (2007) CDD: a conserved domain database for interactive domain family analysis. Nucleic Acids Res. 35, 237–240.CrossRefGoogle Scholar
  19. 19.
    Morozov, S. Yu, Solovyev, A. G. (2003) Triple gene block: modular design of a multifunctional machine for plant virus movement. J. Gen. Virol. 84, 1351–1366.CrossRefGoogle Scholar
  20. 20.
    Park, M. H., Ryu, K. H. (2003) Molecular evidence supporting the classification of Hosta virus X as a distinct species of the genus. Arch. Virol. 148, 2039–2045.CrossRefGoogle Scholar
  21. 21.
    Ryu, K. H., Park, M. H., Lee, J. S. (2002) Occurrence of mosaic disease of Hosta plants caused by Hosta virus X. Plant Pathol. J. 18, 313–316.CrossRefGoogle Scholar
  22. 22.
    Saitou, N., Nei, M. (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425.Google Scholar
  23. 23.
    Sanger, F., Nicklen, S., Coulson, A. R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463–5467.CrossRefGoogle Scholar
  24. 24.
    Solovyev, A. G., Savenkov, E. I., Agranovsky, A. A., Morozov, S. Yu. (1996) Comparisons of the genomic cis-elements and coding regions in RNA β components of the Hordeiviruses Barley Stripe Mosaic Virus, Lychnis Ringspot Virus, and Poa Semilatent Virus. Virology 219, 9–18.CrossRefGoogle Scholar
  25. 25.
    White, K. A., Bancroft, J. B., Mackie, G. A. (1992) Coding capacity determines in vivo accumulation of a defective RNA of clover yellow mosaic virus. J. Virol. 66, 3069–3076.PubMedPubMedCentralGoogle Scholar
  26. 26.
    Wong, S. M., Mahtani, P. H., Lee, K. C., Yu, H. H., Tan, Y., Neo, K. K., Chan, Y., Wu, M. Chang, C. G. (1997) Cymbidium mosaic potexvirus RNA: complete nucleotide sequence and phylogenetic analysis. Arch. Virol. 142, 383–391.CrossRefGoogle Scholar
  27. 27.
    Yamaji, Y., Kagiwada, S., Nakabayashi, H., Ugaki, M., Namba, S. (2001) Complete nucleotide sequence of Tulip virus X (TVX-J): the border between species and strains within the genus Potexvirus. Arch. Virol. 146, 2309–2320.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2012

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Department of Horticulture, Biotechnology and Landscape ArchitectureSeoul Women’s UniversitySeoulKorea

Personalised recommendations