Advertisement

Acta Biologica Hungarica

, Volume 62, Issue 4, pp 376–387 | Cite as

Morphological and Histochemical Investigations of Esophagogastric Tract of a Lizard, Laudakia stellio (Agamidae, Linnaeus 1758)

  • Yücel Başımoğlu KocaEmail author
  • Beyhan Gürcü
Article

Abstract

Histological structures of esophagus and stomach tissue samples of Lacerta stellio have been studied, and glycosaminoglycan (GAG) distribution has been histochemically determined. Histologically, esophagus and stomach of L. stellio are composed of four layers: mucosa, submucosa, muscularis mucosae and serosa. Mucosa of esophagus is covered by simple columnar ciliated epithelium with many mucous secreting goblet cells and contains branched tubular glands.

Stomach of L. stellio is composed of fundus (oral and aboral) and pylorus regions. Mucosa is covered by columnar epithelium. Fundic glands are branched tubular glands while pyloric glands are usually simple tubular glands. In both regions of the stomach, glands are subdivided into three areas as base, neck and isthmus. Both in the esophagus and stomach, muscular layer is in the form of smooth muscle having inner circular and outer longitudinal layers.

According to the results obtained by Alcian Blue (pH 5.8)/Periodic Acid Schiff staining, stomach is similar to esophagus in that neutral mucins and hyaluronic acid (HA) are dominant in isthmus and neck regions of gland tissue of stomach. In the base of the stomach, only neutral mucins have been observed. HA has been observed to be dominant in all other regions of both stomach and esophagus, along with some but not much sulphated GAGs.

Keywords

Histology histochemistry esophagus stomach L. stellio 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ahmed, Y. A., El-Hafez, A. A. E., Zayed, A. E. (2009) Histological and histochemical studies on the esophagus, stomach and small intestines of Varanus niloticus. J. Vet. Anat. 2, 35–48.CrossRefGoogle Scholar
  2. 2.
    Allen, A., Flemström, G. (2005) Gastroduodenal mucus bicarbonate barrier: protection against acid and pepsin. Am. J. Physiol. Cell Physiol. 288, C1–C19.PubMedCrossRefPubMedCentralGoogle Scholar
  3. 3.
    Arellano, J. M., Storch, V., Sarasquete, C. (2002) Ultrastructural study on the intestine of Senegal sole, Solea senegalensis. J. Appl. Ichthyol. 18, 154–158.CrossRefGoogle Scholar
  4. 4.
    Aughey, E., Frye, L. F. (2001) Comparative Veterinary Histology. Manson Publishing Ltd., pp. 105–107.CrossRefGoogle Scholar
  5. 5.
    Bancroft, J. D., Cook, H. C. (1994) Manual of Histological Techniques and Their Diagnostic Application. Churchill Livingstone. London, p. 457.Google Scholar
  6. 6.
    Bansil, R., Turner, B. S. (2006) Mucin structure, aggregation, physiological functions and biomedical applications. Current Opinion in Colloid & Interface Science 11, 164–170.CrossRefGoogle Scholar
  7. 7.
    Domeneghini, C., Ponnelli, R. S., Veggetti, A. (1998) Gut glycoconjugates in Sparus aurata L. (Pisces, Teleostei). A comparative histochemical study in larval and adult ages. Histol. Histopathol. 13, 359–372.PubMedPubMedCentralGoogle Scholar
  8. 8.
    Domeneghini, C., Arrighi, S., Radaelli, G., Bosi, G., Veggetti, A. (2005) Histochemical analysis of glycoconjugate secretion in the alimentary canal Anguilla anguilla L. Acta Histochem. 106, 477–487.PubMedCrossRefPubMedCentralGoogle Scholar
  9. 9.
    Ezeasor, D. N. (1984) Light and electron microscopic studies on the esophageal epithelium of the Rainbow Trout, Salmo gairdneri. Anat. Anz. 155, 71–83.PubMedPubMedCentralGoogle Scholar
  10. 10.
    Ferri, D., Liquori, G. E. (1994) Immunohistochemical investigations on the pyloric glands of the ruin lizard (Podarcis sicula campestris de Betta). Acta Histochem. 96, 96–103.PubMedCrossRefPubMedCentralGoogle Scholar
  11. 11.
    Ferri, D., Liquori, G. E., Natale, L., Santarelli, G., Scillitani, G. (2001) Mucin histochemistry of the digestive tract of the red-legged frog Rana aurora aurora. Acta Histochem. 103, 225–237.PubMedCrossRefPubMedCentralGoogle Scholar
  12. 12.
    Ferri, D., Liquori, G. E. (1992) Characterization of secretory cell glycoconjugates in the alimentary tract of the ruin lizard (Podarcis sicula campestris de Betta) by means of lectin histochemistry. Acta Histochem. 93, 341–349.PubMedCrossRefPubMedCentralGoogle Scholar
  13. 13.
    Gabe, M., Saint-Girons, H. (1972) Contribution al’histologie de l’estomac des lepidossauriens (Reptilis). Zool. Jb. 89, 572–599.Google Scholar
  14. 14.
    Gandhi, N. S., Mancera, R. L. (2008) The structure of glycosaminoglycans and their interaction with proteins. Chem. Biol. Drug Des. 72, 455–482.PubMedPubMedCentralCrossRefGoogle Scholar
  15. 15.
    Geyikoglu, F., Temelli, A., Ozkaral, A. (2002) Muscle fiber types of the tunica muscularis externa at the upper part of the sparrow (Passer domesticus) esophagus. Turk. J. Zool. 26, 217–221.Google Scholar
  16. 16.
    Goldstein, C. D., Jankiewicz, J. J., Desmond, M. E. (1986) Identification of glycosaminoglycans in the chondrocranium of the chick embryo before and at the onset of chondrogenesis. J. Embryol. Exp. Morphol. 93, 29–49.PubMedPubMedCentralGoogle Scholar
  17. 17.
    Humbert, W. R., Kirsch, R., Meister, M. F. (1984) Scanning electron microscopic study of the oesophageal mucous layer in the eel, Anguilla anguilla L. J. Fish Biol. 25, 117–122.CrossRefGoogle Scholar
  18. 18.
    Imai, M., Shibata, T., Izumi, T. (1992) Histological and histochemical investigations on Japanese lizard esophagus. Okajimas Folia Anat. Jpn. 69, 25–34.PubMedCrossRefPubMedCentralGoogle Scholar
  19. 19.
    Imai, M., Shibata, T., Moriguchi, K. (1991) Pepsinogen granules in the esophageal epithelium of the rock snake. Okajimas Folia Anat. Jpn. 68, 231–234.PubMedCrossRefGoogle Scholar
  20. 20.
    Imai, M., Shibata, T., Moriguchi, K., Hayama, H. (1991) Glands distributed in the lamina propria mucosae of the oesophagus in the gecko and Japanese lizard. Okajimas Folia Anat. Jpn. 68, 289–293.PubMedCrossRefPubMedCentralGoogle Scholar
  21. 21.
    Ingelfinger, F. J. (1958) Esophageal motility. Physiol. Rev. 38, 533–584.PubMedCrossRefPubMedCentralGoogle Scholar
  22. 22.
    Liquori, G. E., Mastrodonato, M., Zizza, S., Ferri, D. (2007) Glycoconjugate histochemistry of the digestive tract of Triturus carnifex (Amphibia, Caudata). J. Mol. Hist. 38, 191–199.CrossRefGoogle Scholar
  23. 23.
    Loumbourdis, N. S. (2003) Mucosubstance histochemistry in the esophagus of the lizard Agama stellio stellio. Amphibia-Reptilia 24, 224–229.CrossRefGoogle Scholar
  24. 24.
    Lupa, H. (1977) Histology of the digestive tract. In: Gans, C., Parsons, T. S. (ed.). Biology of the Reptilia. Academic Press, London, pp. 225–302.Google Scholar
  25. 25.
    Morrison, C. M., Wright, J. R. (1999) A study of the histology of the digestive tract of the Nile tilapia. J. Fish Biol. 54, 597–606.CrossRefGoogle Scholar
  26. 26.
    Oinuma, T., Kawano, J. I., Suganuma T. (1991) Glycoconjugate histochemistry of Xenopus laevis fundic gland with special reference to mucouse neck cells during development. Anat. Rec. 230, 502–512.PubMedCrossRefPubMedCentralGoogle Scholar
  27. 27.
    Pedini, V., Scocco, P., Radaelli, G., Fagioli, O., Ceccarelli, P. (2001) Carbohydrate histochemistry of the alimentary canal of the shi drum, umbrina cirrosa L. Anat. Histol. Embryol. 30, 345–349.PubMedCrossRefPubMedCentralGoogle Scholar
  28. 28.
    Pedini, V., Dall’Aglio, C., Parillo, F., Scocco, P. (2004) A lectin histochemical study of the oesophagus of shi drum. J. Fish Biol. 64, 625–631.CrossRefGoogle Scholar
  29. 29.
    Prabhakar, V., Capila, I., Sasisekharan, R. (2009) Glycosaminoglycan characterization methodologies: probing biomolecular interactions. Meth. Mol. Biol. 534, 331–340.Google Scholar
  30. 30.
    Russell, A. L. (1999) Glycosaminoglycan (GAG) deficiency in protective barrier as an underlying, primary cause of ulcerative colitis, Crohn’s disease, interstitial cystitis and possibly Reiter’s syndrome. Medical Hypothesis 52, 297–301.CrossRefGoogle Scholar
  31. 31.
    Sancar-Bas, S., Kaptan, E., Sengezer-Inceli, M., Sezen, A., Us, H. (2009) Glycoconjugate histochemistry in the Fundic stomach and small intestine of the frog (Rana ridibunda). IUFS J. Biol. 68, 93–104.Google Scholar
  32. 32.
    Sartori, S. S. R., Nogueira, K. O. P. C., Rocha, A. S. R., Neves, C. A. (2010) Morphology of the stomach of the tropical house gecko Hemidactylus mabouia (Squamata: Gekkonidae). Acta Zoologica, 1–8.Google Scholar
  33. 33.
    Scott, J. E. (1996) Alcian blue. Now you see it, now you don’t. Eur. J. Oral Sci. 104, 2–9.CrossRefGoogle Scholar
  34. 34.
    Selvan, P. S., Ushakumary, S., Ramesh, G. (2008) Studies on the histochemistry of the proventriculus and gizzard of post-hatch guinea fowl (Numida meleagris). Inter. J. Poultry Scien. 7, 1112–1116.CrossRefGoogle Scholar
  35. 35.
    Sheahan, D. G., Jervis, H. R. (1976) Comparative histochemistry of gastrointestinal mucosubstances. Am. J. Anat. 146, 103–131.PubMedCrossRefPubMedCentralGoogle Scholar
  36. 36.
    Smith, L. S. (1989) Digestive functions in teleost fish. In: J. E. Halvert (ed.), Fish Nutrition Academic Press, San Diego, pp. 331–421.Google Scholar
  37. 37.
    Starck, J. M., Beese, K. (2002) Structural flexibility of the small intestine and liver of garter snakes in response to feding and fasting. J. Exp. Biol. 205, 1377–1388.PubMedPubMedCentralGoogle Scholar
  38. 38.
    Suganuma, T., Katsuyama, T., Tsukahara, M., Tatematsu, M., Sakakura, Y., Murata, F. (1981) Comparative histochemical study of alimentary tracts with special reference to the mucous neck cell of the stomach. Am. J. Anat. 161, 219–238.PubMedCrossRefPubMedCentralGoogle Scholar
  39. 39.
    Suprasert, A., Fujioka, T., Yamad, K. (1987) The histochemistry of glycoconjugates in the colonic epithelium of the chicken. Histochemistry 86, 491–497.PubMedCrossRefPubMedCentralGoogle Scholar
  40. 40.
    Suzuki, S., Mizutani, A., Koike, Y., Kato, M., Yoshida, K., Kimata, K. (1991) Glycosaminoglycan chains of proteoglycans: approaches to the study of their structure and function. Pure Appl. Chem. 63, 545–554.CrossRefGoogle Scholar
  41. 41.
    Taylor, P. M., Tyler, M. J. (1986) Pepsin in the toad Bufo marinus. Comp. Biochem. Physiol. 84, 669–672.CrossRefGoogle Scholar
  42. 42.
    Tuckett, F., Morris-Kay, G. (1988) Alcian blue staining of glycosaminoglycans in embrionic material: Effect of different fixatives. Histochem. J. 20, 174–182.PubMedCrossRefPubMedCentralGoogle Scholar
  43. 43.
    Uriona, T. J., Farmer, C. G., Dazely, J., Clayton, F., Moore, J. (2005) Structure and function of the esophagus of the American alligator (Alligator mississippiensis). J. Exp. Biol. 208, 3047–3053.PubMedCrossRefPubMedCentralGoogle Scholar
  44. 44.
    Ünal, G., Çetinkaya, O., Kankaya, E., Elp, M. (2001) Histological study of the organogenesis of the digestive system and swim bladder of the Chalcalburnus tarichi Palas, 1811 (Cyprinidae). Turk. J. Zool. 25, 217–228.Google Scholar
  45. 45.
    Yamabayashi, S. (1987) Periodic acid-Sciff-Alcian blue: A method for the differential staining of glycoproteins. Histochem. J. 19, 565–571.PubMedCrossRefPubMedCentralGoogle Scholar
  46. 46.
    Yoshida, M. (2001) A light microscope study of the distribution of muscle in the frog esophagus and stomach. J. Smooth Muscle Res. 37, 95–104.PubMedCrossRefPubMedCentralGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2011

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Department of Biology, Faculty of Sciences and ArtAdnan Menderes UniversityAydinTurkey
  2. 2.Department of Biology, Faculty of Sciences and ArtCelal Bayar UniversityManisaTurkey

Personalised recommendations