Nano Silver Treatment is Effective in Reducing Bacterial Contaminations of Araucaria Excelsa R. Br. var. Glauca Explants

Abstract

The downside of plant tissue culture techniques is an unwanted microbial contamination. Elimination of contaminants is the first step of any successful investigation on plant tissue culture. Preliminary experiments on Araucaria excelsa R. Br. var. glauca (Norfolk-Island pine) (syn.: A. heterophylla) showed that most common decontaminants could not successfully eliminate the contamination. Therefore, nano silver (NS) colloids were evaluated for controlling contamination. Treatments were included soaking the explants in NS solution or adding NS to the culture medium. Explants were cultured on MS medium supplemented with appropriate growth regulators for their establishment. Results showed that surface sterilization followed by treatment with 200 mg l−1 of NS with soaking time of 180 min reduced the bacterial contamination from 61.5% to 11.3% and adding 400 mg l−1 NS to the medium reduced the bacterial contamination from 81.25% to 18.75%. Nano silver could be applied without adverse effects on plant growth and development. This is the first report on in vitro establishment of A. excelsa R. Br. using NS to reduce bacterial infections.

References

  1. 1.

    Abdi, G. H., Salehi, H., Khosh-Khui, M. (2008) Nano silver: a novel nanomaterial for removal of bacterial contaminants in valerian (Valeriana officinalis L.) tissue culture. Acta Physiol. Plant. 30, 709–714.

    CAS  Article  Google Scholar 

  2. 2.

    Baker, C., Pradhan, A., Pakstis, L., Pochan, D. J., Shah, S. I. (2005) Synthesis and antibacterial properties of silver nanoparticles. J. Nanosci. Technol. 5, 244–249.

    CAS  Google Scholar 

  3. 3.

    Batarseh, K. I. (2004) Anomaly and correlation of killing in the therapeutic properties of silver(I) chelating with glutamic and tartaric acids. J. Antimicrob. Chemoth. 54, 546–548.

    CAS  Article  Google Scholar 

  4. 4.

    Bragg, P. D., Rannie, D. J. (1974) The effect of silver ions on the respiratory chain of E. coli. Can. J. Microbiol. 20, 883–889.

    CAS  Article  Google Scholar 

  5. 5.

    Braydich-Stolle, L., Hussain, S., Schlager, J. J., Hofmann, M. C. (2005) In vitro cytotoxicity of nanoparticles in mammalian germline stem cells. Toxicol. Sci. 88, 412–419.

    CAS  Article  Google Scholar 

  6. 6.

    Constantine, D. R. (1986) Micropropagation in the commercial environment. In: Withers, L., Alderson, P. G. (eds) Plant tissue culture and its agricultural applications Butterworth, London, pp. 175–186.

    Chapter  Google Scholar 

  7. 7.

    Dodds, J. H., Roberts, W. L. (1981) Some inhibitory effectors on gentamicin on plant tissue culture. In Vitro 17, 467–470.

    CAS  Article  Google Scholar 

  8. 8.

    Eapen, S., George, L. (1997) Plant regeneration from peduncle segments of oil seed brassica species: influence of silver nitrate and silver thiosulfate. Plant Cell Tiss. Org. Cult. 51, 229–232.

    CAS  Article  Google Scholar 

  9. 9.

    Falkiner, F. R. (1990) The criteria for choosing an antibiotic for control of bacteria in plant tissue culture in TCL. Assoc. Plant Tiss. Cult. Newsl. 60, 13–23.

    Google Scholar 

  10. 10.

    Geong, Y., Hwang, H., Hi, S. C. (2005) Antibacterial properties of padded PP/PE nonwovens incorporating nano-sized silver. Colloids J. Mater. Sci. 40, 5413–5418.

    Article  Google Scholar 

  11. 11.

    Giri, C. C., Shyamkmar, B., Anjaneylnu, C. (2004) Progresses in tissue culture, genetic transformation and application of biotechnology to trees: an overview. Trees 18, 115–135.

    Article  Google Scholar 

  12. 12.

    Gyulai, G. R., Láposi, C., Herschbach, A., Veres, Gy., Fábián, L., Waters, Jr., Rennenberg, H. (2011) Conservation genetics (1710-2010) - Cloning of living fossils: Micropropagation of the oldest Hungarian black locust tree (Robinia pseudoacacia) planted in 1710 (Bábolna, Hungary). In: G. Gyulai, G. (ed.). Plant Archaeogenetics. Chapter 10. Nova Sci Publisher Inc., New York, USA.

    Google Scholar 

  13. 13.

    Harry, I. S., Thorpe, T. A. (1990) Special problems and prospects in the propagation of woody species, in plant aging basic and applied approaches. Plenum. New York, pp. 67–74.

    Google Scholar 

  14. 14.

    Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.

    CAS  Article  Google Scholar 

  15. 15.

    Nomiya, K., Yoshizawa, A., Tsukagoshi, K., Kasuga, N. C., Hirakava, S., Watanabe, J. (2004) Synthesis and structural characterization of silver(I), aluminium(III) and cobalt(II) complexes with 4-isopropyltropolone (hinokitiol) showing noteworthy biological activities. Action of silver(I)-oxygen bonding complexes on the antimicrobial activities. J. Inorg. Biochem. 98, 46–60.

    CAS  Article  Google Scholar 

  16. 16.

    Park, H. J., Kim, S. H., Kim, H. J., Choi, S. (2006) A new composition of nanosized silica-silver for control of various plant diseases. Plant Pathol. J. 22, 295–302.

    Article  Google Scholar 

  17. 17.

    Purnhauser, L., Medgyesy, P., Czakó, M., Dix, P. J., Márton, L. (1987) Stimulation of shoot regeneration in Triticum aestivum and Nicotiana plumbaginifolia Viv. tissue cultures using the ethylene inhibitor AgNO3. Plant Cell Report 6, 1–4.

    CAS  Article  Google Scholar 

  18. 18.

    Russell, A. D., Hugo, W. B. (1994) Antimicrobial activity and action of silver. Prog. Med. Chem. 31, 351–371.

    CAS  Article  Google Scholar 

  19. 19.

    Sarmast, M. K., Salehi, H., Khosh-Khui, M. (2009) Using plagiotropic shoot explant in tissue culture of Araucaria excelsa R. Br. var. glauca. Adv. Environ. Biol. 3, 194–294.

    Google Scholar 

  20. 20.

    Sarmast, M. K., Salehi, H., Ramezani, A., Abolimoghadam, A. A., Niazi, A., Khosh-Khui, M. (2011) RAPD fingerprint to appraise the genetic fidelity of in vitro propagated Araucaria excelsa R. Br. var. glauca plantlets. Mol. Biotechnol. DOI 10.1007/s12033-011-9421-7.

    Google Scholar 

  21. 21.

    Sehgal, L., Sehgal, O. P., Khosla, P. K. (1989) Micropropagation of Araucaria columnaris Hook. Ann. Sci. Forest 46, 158–160.

    Article  Google Scholar 

  22. 22.

    Shrivastava, S., Bera, T., Roy, A., Singh, G., Ramachandrarao, P., Debabrata, D. (2007) Characterization of enhanced antibacterial effects of novel silver nanoparticles. Nanotechnology 18, 225103. (9 pp).

    Article  Google Scholar 

  23. 23.

    Sondi, I., Salopek-Sondi, B. (2004) Silver nano particles as antimicrobial agent: A case study on E. coli as a model for Gram-negative bacteria. J. Colloid Interface Sci. 275, 177–182.

    CAS  Article  Google Scholar 

  24. 24.

    Taiz, L., Zeiger, E. (2006) Plant Physiology. Sinauer Assoc. Inc., 4 ed. 700 p.

    Google Scholar 

  25. 25.

    Teixeira da Silva, G. A., Duong, T., Michi, T., Seiichi, F. (2003) The effect of antibiotics on the in vitro growth response of chrysanthemum and tobacco stem transverse thin cell layers (tTCLs). Sci. Hortic. 97, 397–410.

    CAS  Article  Google Scholar 

  26. 26.

    Wainwright, M., Grayston, S. J., de Jong, P. (1986) Adsorption of insoluble compounds by mycelium of the fungus Mucor flavus. Enzyme Micro. Technol. 8, 597–600.

    CAS  Article  Google Scholar 

  27. 27.

    Zhang, P., Phansiri, S., Kaerlas, J. P. (2001) Improvement of cassava shoot organogenesis by the use of silver nitrate in vitro. Plant Cell Tiss. Org. Cult. 67, 47–54.

    CAS  Article  Google Scholar 

  28. 28.

    Zhang, W., Qiao, X., Chen, J. (2007) Synthesis of nanosilver. Colloids and Surfaces A: Phys. Eng. Asp. 299, 22–28.

    CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to H. Salehi.

Rights and permissions

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and Permissions

About this article

Cite this article

Sarmast, M.K., Salehi, H. & Khosh-Khui, M. Nano Silver Treatment is Effective in Reducing Bacterial Contaminations of Araucaria Excelsa R. Br. var. Glauca Explants. BIOLOGIA FUTURA 62, 477–484 (2011). https://doi.org/10.1556/ABiol.62.2011.4.12

Download citation

Keywords

  • Contamination
  • nanobiotechnology
  • Norfolk-Island pine
  • silver nanoparticle
  • tissue culture