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Acta Biologica Hungarica

, Volume 62, Issue 3, pp 265–278 | Cite as

Effects of Methyl Jasmonate on Accumulation of Flavonoids in Seedlings of Common Buckwheat (Fagopyrum Esculentum Moench)

  • M. HorbowiczEmail author
  • W. Wiczkowski
  • Danuta Koczkodaj
  • M. Saniewski
Article

Abstract

The jasmonates, which include jasmonic acid and its methyl ester (MJ), play a. central role in regulating the biosynthesis of many secondary metabolites, including flavonoids, and also are signaling molecules in environmental stresses. Synthesis of anthocyanins pigments is a. final part of flavonoids pathway route. Accumulation of the pigments in young seedlings is stimulated by various environmental stresses, such as high-intensity light, wounding, pathogen attack, drought, sugar and nutrient deficiency. The anthocyanins take part in defense system against excess of light and UV-B light, and therefore it is probably main reason why young plant tissues accumulate enlarged levels of the pigments. The effects of exogenously applied MJ on level of anthocyanins, glycosides of apigenin, luteolin, quercetin and proanthocyanidins in seedlings of common buckwheat (Fagopyrum esculentum Moench) were studied. MJ decreased contents of all the found cyanidin glycosides and its aglycone in hypocotyls of buckwheat seedlings. However contents of particular anthocyanins in cotyledons of buckwheat seedlings treated with the plant hormone were not significantly different from the control. Applied doses of MJ did not affect levels of quercetin, apigenin and luteolin glycosides in the analyzed parts of buckwheat seedlings: cotyledons and hypocotyls. On the other hand, treatment of buckwheat seedlings with MJ clearly stimulated of proanthocyanidins biosynthesis in hypocotyls. We suggest that methyl jasmonate induces in hypocotyls of buckwheat seedlings the leucocyanidin reductase or anthocyanidin reductase, possible enzymes in proanthocyanidins synthesis, and/or inhibits anthocyanidin synthase, which transforms leucocyanidin into cyanidin. According to our knowledge this is the first report regarding the effect of methyl jasmonate on enhancing the accumulation of proanthocyanidins in cultivated plants.

Keywords

Anthocyanins common buckwheat flavonoids methyl jasmonate proanthocyanidins 

Abbreviations

Api-8C-Glu

vitexin

Api-6C-Glu

isovitexin

Lut-8C-Glu

orientin

Lut-6C-Glu

isoorientin

Quer-3-Gal-Rha

quercetin 3-galactorhamnoside

Quer-3-Glu

isoquercitrin

Quer-3-Glu

Rha - rutin

Cy-3-Gal

cyanidin 3-galactoside

Cy-3-Gal-Rha

cyanidin 3-galactorhamnoside

Cy-3-Glu

cyanidin 3-Glucoside

Cy-3-Glu-Rha

cyanidin 3-glucorhamnoside

PA

proanthocyanidins

MJ

methyl jasmonate

JA

jasmonic acid

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© Akadémiai Kiadó, Budapest 2011

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • M. Horbowicz
    • 1
    Email author
  • W. Wiczkowski
    • 2
  • Danuta Koczkodaj
    • 1
  • M. Saniewski
    • 3
  1. 1.Department of Plant Physiology and Genetics, Institute of BiologyUniversity of PodlasieSiedlcePoland
  2. 2.Institute of Animal Reproduction and Food ResearchPolish Academy of SciencesOlsztynPoland
  3. 3.Research Institute of Pomology and FloricultureSkierniewicePoland

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