Effects of Methyl Jasmonate on Accumulation of Flavonoids in Seedlings of Common Buckwheat (Fagopyrum Esculentum Moench)

Abstract

The jasmonates, which include jasmonic acid and its methyl ester (MJ), play a. central role in regulating the biosynthesis of many secondary metabolites, including flavonoids, and also are signaling molecules in environmental stresses. Synthesis of anthocyanins pigments is a. final part of flavonoids pathway route. Accumulation of the pigments in young seedlings is stimulated by various environmental stresses, such as high-intensity light, wounding, pathogen attack, drought, sugar and nutrient deficiency. The anthocyanins take part in defense system against excess of light and UV-B light, and therefore it is probably main reason why young plant tissues accumulate enlarged levels of the pigments. The effects of exogenously applied MJ on level of anthocyanins, glycosides of apigenin, luteolin, quercetin and proanthocyanidins in seedlings of common buckwheat (Fagopyrum esculentum Moench) were studied. MJ decreased contents of all the found cyanidin glycosides and its aglycone in hypocotyls of buckwheat seedlings. However contents of particular anthocyanins in cotyledons of buckwheat seedlings treated with the plant hormone were not significantly different from the control. Applied doses of MJ did not affect levels of quercetin, apigenin and luteolin glycosides in the analyzed parts of buckwheat seedlings: cotyledons and hypocotyls. On the other hand, treatment of buckwheat seedlings with MJ clearly stimulated of proanthocyanidins biosynthesis in hypocotyls. We suggest that methyl jasmonate induces in hypocotyls of buckwheat seedlings the leucocyanidin reductase or anthocyanidin reductase, possible enzymes in proanthocyanidins synthesis, and/or inhibits anthocyanidin synthase, which transforms leucocyanidin into cyanidin. According to our knowledge this is the first report regarding the effect of methyl jasmonate on enhancing the accumulation of proanthocyanidins in cultivated plants.

Abbreviations

Api-8C-Glu:

vitexin

Api-6C-Glu:

isovitexin

Lut-8C-Glu:

orientin

Lut-6C-Glu:

isoorientin

Quer-3-Gal-Rha:

quercetin 3-galactorhamnoside

Quer-3-Glu:

isoquercitrin

Quer-3-Glu:

Rha - rutin

Cy-3-Gal:

cyanidin 3-galactoside

Cy-3-Gal-Rha:

cyanidin 3-galactorhamnoside

Cy-3-Glu:

cyanidin 3-Glucoside

Cy-3-Glu-Rha:

cyanidin 3-glucorhamnoside

PA:

proanthocyanidins

MJ:

methyl jasmonate

JA:

jasmonic acid

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Correspondence to M. Horbowicz.

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Horbowicz, M., Wiczkowski, W., Koczkodaj, D. et al. Effects of Methyl Jasmonate on Accumulation of Flavonoids in Seedlings of Common Buckwheat (Fagopyrum Esculentum Moench). BIOLOGIA FUTURA 62, 265–278 (2011). https://doi.org/10.1556/ABiol.62.2011.3.6

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Keywords

  • Anthocyanins
  • common buckwheat
  • flavonoids
  • methyl jasmonate
  • proanthocyanidins