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Acta Biologica Hungarica

, Volume 62, Issue 3, pp 316–327 | Cite as

Induced Proteolysis within the Bird Cherry Leaves Evoked by Rhopalosiphum PadiL. (Hemiptera, Aphidoidea)

  • H. SytykiewiczEmail author
  • P. Czerniewicz
  • Iwona Sprawka
  • Sylwia Goławska
  • G. Chrzanowski
  • B. Leszczyński
Article

Abstract

The objectives of this study were to elucidate the impact of bird cherry-oat aphid (Rhopalosiphum padi L.) feeding on functioning of the proteolytic machinery in bird cherry leaves. Biochemical analyses proved that R. padi feeding in tissues of primary host stimulated activity of the two major fractions of proteinases (extracted at the optimal pH values: 5.0 and 7.0). Additionally, it has been demonstrated that aphids’ feeding on bird cherry led to a. decline in levels of albumins and globulins (main protein fractions in P. padus leaves). The opposite tendency, regarding the amounts of these protein fractions was ascertained at the phase of disappearance of R. padi population on tested shoots. Furthermore, it is reported that an increase in activity of the analysed enzymes and a. decline in the content of tested protein fractions, were proportional to density of aphid individuals developing on P. padus side shoots. It is hypothesized that long-term R. padi feeding may lead to intensifying the catabolic processing of proteins by the activated proteolytic machinery in bird cherry leaves. The multi-level biological functions of endogenous plant proteinases and their significance in triggering the defense reactions in aphid-infested plant tissues are discussed.

Keywords

Proteolysis proteinases albumins globulins aphid-plant interactions 

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© Akadémiai Kiadó, Budapest 2011

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • H. Sytykiewicz
    • 1
    Email author
  • P. Czerniewicz
    • 1
  • Iwona Sprawka
    • 1
  • Sylwia Goławska
    • 1
  • G. Chrzanowski
    • 1
  • B. Leszczyński
    • 1
  1. 1.Department of Biochemistry and Molecular BiologySiedlce University of Natural Sciences and HumanitiesSiedlcePoland

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