Acta Biologica Hungarica

, Volume 62, Issue 2, pp 182–193 | Cite as

Physiological and Anatomical Adaptations Induced by Flooding in Cotula Coronopifolia

  • A. SmaouiEmail author
  • Jihène Jouini
  • M. Rabhi
  • G. Bouzaien
  • A. Albouchi
  • C. Abdelly


Cotula coronopifolia is a wild annual Asteraceae that grows in periodically-flooded prone environments and seems highly tolerant to periodic flooding. Seedlings of about 15 cm were collected directly from the edge of Soliman sabkha (N-E Tunisia, semi-arid stage) and grown under greenhouse conditions. Two treatments were considered: drainage and flooding. After 56 days of treatment, flooded plants showed a pronounced growth increase. This performance was essentially associated with significant increment in biomass production of both shoots and roots (about 220% of the control). The appropriate response to flooding was also characterized by the ability of the species to maintain its water status under such conditions. Neither water content nor water potential showed a significant variation as compared to those of non-flooded plants. However, transpiration rate decreased slightly but significantly in flooded plants (from 0.86 to 0.64 mmol H2O m−2 s−1). Na+ and K+ concentrations were practically maintained under waterlogging conditions, except a significant increase of Na+ content in roots of flooded plants (157% of the control). These responses were concomitant with maintenance of photosynthetic rate. However, the contents of chlorophylls a and b increased to 167% and 295%, respectively. It seems that the enhancement in these photosynthetic pigments together with a significant improvement in water use efficiency (from 4.66 to 6.07 mmol CO2 mol−1 H2O) allowed to the species to compensate the decrease in photosynthetic rate. At the anatomical level, this species responded to flooding by a significant development of its root aerenchyma (+63%) and an increase in the lignification of its stem xylem tissues (+37%). Based on the presented data, the plant fitness under flooding conditions was a result of dynamic readjustment of several morphological, physiological, and anatomical adaptive traits. Flood requirement together with salt tolerance are responsible for the predominance of C. coronopifolia in a large area in its natural biotope where most plants cannot tolerate interactive effects of flooding and salinity.


Aerenchyma Cotula coronopifolia photosynthesis waterlogging water relations 


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  1. 1.
    Barrett-Lennard, E. G. (2003) The interaction between waterlogging and salinity in higher plants: causes, consequences and implications. Plant Soil 253, 35–54.CrossRefGoogle Scholar
  2. 2.
    Blom, C. W. P. M. (1999) Adaptations to flooding stress: from plant community to molecule. Plant Biol. 1, 261–273.CrossRefGoogle Scholar
  3. 3.
    Blom, C. W. P. M., Voesenek, L. A. C. J. (1996) Flooding: the survival strategies of plants. Tree 11, 290–295.PubMedGoogle Scholar
  4. 4.
    Costa, J. C., Neto, C., Arsénio, P., Capelo, J. (2009) Geographic variation among Iberian communities of the exotic halophyte Cotula coronopifolia. Bot. Helv. 119, 53–61.CrossRefGoogle Scholar
  5. 5.
    Gomes, S. A. R., Kozlowski, T. T. (1980) Growth responses and adaptations of Fraxinus pennsylvanica seedlings to flooding. Plant Physiol. 66, 267–271.CrossRefGoogle Scholar
  6. 6.
    Grusak, M. A. (1995) Whole-root iron (III)-reductase activity the life cycle of iron grown Pisum sativum L. (Fabaceae): relevance to the iron nutrition of developing seeds. Planta 197, 111–117.CrossRefGoogle Scholar
  7. 7.
    Halit, Y., Mehmet, E. C., Aliskan, B., Soner, S., Musa, S. (2006) Some physiological and growth responses of watermelon [Citrullus lanatus (Thunb.) Matsum. and Nakai] grafted onto Lagenaria siceraria to flooding. Environ. Exp. Bot. 58, 1–8.Google Scholar
  8. 8.
    Insausti, P., Grimoldi, A.A., Chaneton, E.J., Vasellati, V. (2001) Flooding induces a suite of adaptive plastic responses in the grass Paspalumdilatatum. New Phytol. 152, 291–299.CrossRefGoogle Scholar
  9. 9.
    Jackson, M. B., Colmer, T. D. (2005) Response and adaptation by plants to flooding stress. Ann. Bot. 96, 501–505.CrossRefGoogle Scholar
  10. 10.
    Jackson, M. B. (1982) Ethylene as a growth promoting hormone under flooded conditions. In: Wareing, P. F. (ed.) Plant Growth Regulators. Academic Press, London, pp. 291–301.Google Scholar
  11. 11.
    Jackson, M. B., Drew, M. (1984) Effects of flooding on growth and metabolism of herbaceous plants. In: Kozlowski, T. T. (ed.) Flooding and Plant Growth. Academic Press, London, pp. 47–128.CrossRefGoogle Scholar
  12. 12.
    Jackson, M. B., Armstrong, W. (1999) Formation of aerenchyma and the processes of plant ventilation in relation to soil flooding and submergence. Plant Biol. 1, 274–287.CrossRefGoogle Scholar
  13. 13.
    Kolodynska, A., Pigliucci, M. (2003) Multivariate responses to flooding in Arabidopsis: an experimental evolutionary investigation. Funct. Ecol. 17, 131–140.CrossRefGoogle Scholar
  14. 14.
    Kozlowski, T. T. (1997) Responses of woody plants to flooding and salinity. Tree Physiol. Monogr. 1, 1–29.Google Scholar
  15. 15.
    Laan, P., Tosserams, M., Blom, C. W. P. M., Veen, B. W. (1990) Internal oxygen transport in Rumex species and its significance for respiration under hypoxic conditions. Plant Soil 122, 39–46.CrossRefGoogle Scholar
  16. 16.
    Li, S., Pezeshki, S. R., Goodwin, S. (2004) Effects of soil moisture regimes on photosynthesis and growth in cattail (Typha latifolia). Acta Oecol. 25, 17–22.CrossRefGoogle Scholar
  17. 17.
    Matsumoto, K., Ohta, T., Tanaka, T. (2005) Dependence of stomatal conductance on leaf chlorophyll concentration and meteorological variables. Agric. For. Meteorol. 132, 44–57.CrossRefGoogle Scholar
  18. 18.
    Mielke, M. S. (2005) Some photosynthetic and growth responses of Annona glabra L. seedlings to soil flooding. Acta Bot. Bras. 19, 905–911.CrossRefGoogle Scholar
  19. 19.
    Naidoo, G., Naidoo, S. (1992) Waterlogging responses of Sporobolus virginicus (L.) Kunth. Oecologia 90, 445–450.CrossRefGoogle Scholar
  20. 20.
    Pezeshki, S. R. (2001) Wetland plant responses to soil flooding. Environ. Exp. Bot. 46, 299–312.CrossRefGoogle Scholar
  21. 21.
    Salter, J., Morris, K., Paul, C. E., Bailey, Paul I. Boon (2007) Interactive effects of salinity and water depth on the growth of Melaleuca ericifolia Sm. (Swamp paperbark) seedlings. Aquat. Bot. 86, 213–222.CrossRefGoogle Scholar
  22. 22.
    Sauter, M. (2000) Rice in deep water: “How to take heed against a sea of troubles?” Naturwissenschaften 87, 289–303.CrossRefGoogle Scholar
  23. 23.
    Scholander, P. F., Hammel, H. T., Bradstreet, E. D., Hemmingsen, E. D. (1965) Sap pressure in vascular plants. Science 148, 339–346.CrossRefGoogle Scholar
  24. 24.
    Smith, K. A, Russell, R. S. (1969) Occurrence of ethylene, and its significance, in anaerobic soil. Nature 222, 769–771.CrossRefGoogle Scholar
  25. 25.
    Thomson, C. J., Armstrong, W., Waters, I., Greenway, H. (1990) Aerenchyma formation and associated oxygen movement in seminal and nodal roots of wheat. Plant Cell Environ. 13, 395–403.CrossRefGoogle Scholar
  26. 26.
    Topa, M. A., Cheeseman, J. M. (1992) Carbon and phosphorus partitioning in Pinus serotina seedlings growing under hypoxic and low-phosphorus conditions. Tree Physiol. 10, 195–207.CrossRefGoogle Scholar
  27. 27.
    Trought, M. C. T., Drew, M. C. (1980) The development of waterlogging damage in young wheat plants in anaerobic solution cultures. J. Exp. Bot. 31, 1573–1585.CrossRefGoogle Scholar
  28. 28.
    Videmesk, U., Turk, B., Vodnik, D. (2006) Root aerenchyma — formation and function. Acta Agric. Slov. 87, 445–453.Google Scholar
  29. 29.
    Visser, E. J. W., Colmer, T. D., Blom, C. W. P. M., Voesenek, L. A. C. J. (2000) Changes in growth, porosity, and radial oxygen loss from adventitious roots of selected mono- and dicotyledonous wetland species with contrasting types of aerenchyma. Plant Cell Environ. 23, 1237–1245.CrossRefGoogle Scholar
  30. 30.
    Wample, R. L., Thornton, R. K. (1984) Differences in the response of sunflower (Helanthus annuus) subjected to flooding and drought stress. Physiol. Plant. 61, 611–616.CrossRefGoogle Scholar
  31. 31.
    Wassen, M. J., Peeters, W. H. M., Venterink, H. O. (2002) Patterns in vegetation, hydrology, and nutrient availability in an undisturbed river floodplain in Poland. Plant Ecol. 165, 27–43.CrossRefGoogle Scholar
  32. 32.
    Li, M., Yang, D., Li, W. (2007) Leaf gas exchange characteristics and chlorophyll fluorescence of three wetland plants in response to long-term soil flooding. Photosynthetica 45, 222–228.CrossRefGoogle Scholar
  33. 33.
    Ye, Y., Nora, F. Y., Tam, A., Wong, Y. S., Lu, C.Y. (2003) Growth and physiological responses of two mangrove species (Bruguiera gymnorrhiza and Kandelia candel) to waterlogging. Environ. Exp. Bot. 49, 209–221.CrossRefGoogle Scholar

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© Akadémiai Kiadó, Budapest 2011

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • A. Smaoui
    • 1
    Email author
  • Jihène Jouini
    • 1
  • M. Rabhi
    • 1
  • G. Bouzaien
    • 2
  • A. Albouchi
    • 2
  • C. Abdelly
    • 1
  1. 1.Laboratory of Extremophile Plants (LPE)Biotechnology Centre of Borj-CedriaHammam-LifTunisia
  2. 2.Laboratory of AgrosylvopastoralismNational Institute of Research in Rural Engineering, Waters and ForestsArianaTunisia

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