Acta Biologica Hungarica

, Volume 62, Issue 2, pp 171–181 | Cite as

Non-TIR-NBS-LRR Resistance Gene Analogs in Apricot (Prunus Armeniaca L.)

  • Á. GutermuthEmail author
  • Zsuzsanna György
  • A. Hegedűs
  • A. Pedryc


Genes encoding for proteins with nucleotide-binding site and leucine-rich repeat motifs (NBS-LRR) have been suggested to play a general role in plant defence mechanism. In Prunus species, many TIR (Toll / Interleukin-1 Receptor), and only very few non-TIR sequences were identified, which was explained either by the unequal distribution of TIR/non-TIR sequences in the Prunus genome or by the incapability of primers in the amplification of non-TIR RGAs. The objective of this work was to check whether a new semi-nested PCR strategy can be developed for the targeted isolation of non-TIR-NBS-LRR Resistance Gene Analog (RGA) sequences from apricot. Three primers (CUB-P-loop F, CUB-Kin2 F and CUB-HD R) were designed, from which CUB-Kin2 F and CUB-HD R were constructed to anneal selectively to the non-TIR sequences. A colony Polymerase Chain Reaction (PCR) indicated that out of the 96 clones tested 28 showed amplification using the newly developed primers, while no amplification occurred when using the formerly described primers. Half of the 28 positive clones were sequenced and they turned out to represent 11 different non-TIR RGA sequences. A phylogenetic analysis was carried out based on an alignment containing 293 Rosaceae and 21 non-Rosaceaa sequences. A significantly higher ratio (91%) of non-TIR sequences were arranged in multi-genera clades than that of (57%) the TIR groups confirming that non-TIR sequences might be of more ancient origin than TIR sequences.


Apricot Prunus armeniaca L. resistance gene analogs RGA non-TIR-NBS-LRR 


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  1. 1.
    Di Gaspero, G., Cipriani, G. (2003) Nucleotide binding site/leucine-rich repeats, Pto-like and receptor- like kinases related to disease resistance in grapevine. Mol. Gen. Genomics 269, 612–623.CrossRefGoogle Scholar
  2. 2.
    Dondini, L., Costa, F., Tataranni, G., Tartarini, S., Sansavini, S. (2004) Cloning of apricot RGA (Resistance Gene Analogs) and development of molecular markers associated with Sharka (PPV) resistance. J. Hortic. Sci. Biotech. 79, 729–734.CrossRefGoogle Scholar
  3. 3.
    Gentzbittel, L., Mouzeyar, S., Badaoui, S., Mestries, E., Vear, F., De Labrouhe, D. T., Nicolas, P. (1998) Cloning of molecular markers for disease resistance in sunflower, Helianthus annuus L. Theor. Appl. Genet. 96, 519–525.CrossRefGoogle Scholar
  4. 4.
    Higgins, D., Thompson, J., Gibson, T., Thompson, J. D., Higgins, D., Gibson, T. J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic. Acids Res. 22, 4673–4680.CrossRefGoogle Scholar
  5. 5.
    The Arabidopsis Genome Initiative (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408, 796–816.CrossRefGoogle Scholar
  6. 6.
    Kanazin, V., Marek, L. F., Shoemaker, R. C. (1996) Resistance-gene analogs are conserved and clustered in soybean. Proc. Natl. Acad. Sci. 93, 11746–11750.CrossRefGoogle Scholar
  7. 7.
    Lalli, D. A., Decroocq, V., Blenda, V., Shurdi-Levraud, V., Garay, L., Le Gall, O., Damsteegt, V., Reighard, G. L., Abbott, A. G. (2005) Identification and mapping of resistance gene analogs (RGAs) in Prunus: a resistance map for Prunus. Theor. Appl. Genet. 111, 1504–1513.CrossRefGoogle Scholar
  8. 8.
    Leister, D., Ballvora, A., Salamini, F., Gebhardt, C. (1996) A PCR based approach for isolating pathogen resistance genes from potato with potential for wide application in plants. Nat. Genet. 14, 421–429.CrossRefGoogle Scholar
  9. 9.
    McHale, L., Tan, X., Koehl, P., Michelmore, R. W. (2006) Plant NBS-LRR proteins: adaptable guards. Genome Biol. 7, 212.CrossRefGoogle Scholar
  10. 10.
    Meyers, B. C., Kaushik, S., Nandety, R. S. (2005) Evolving disease resistance genes. Curr. Opin. Plant Biol. 8, 129–134.CrossRefGoogle Scholar
  11. 11.
    Meyers, B. C., Dickerman, A. W., Michelmore, R. W., Sivaramakrishnan, S., Sobral, B. W., Young, N. D. (1999) Plant disease resistance genes encode members of an ancient and diverse protein family within the nucleotide-binding superfamily. Plant J. 20, 317–332.CrossRefGoogle Scholar
  12. 12.
    Samuelian, S. K., Baldo, A. M., Pattison, J. A., Weber, C. A. (2008) Isolation and linkage mapping of NBS-LRR resistance gene analogs in red raspberry (Rubus idaeus L.) and classification among Rosaceae NBS-LRR genes. Tree Genet. Genomes 4, 881–896.CrossRefGoogle Scholar
  13. 13.
    Soriano, J. M., Vilanova, S., Romero, C., Llácer, G., Badenes, M. L. (2005) Characterization and mapping of NBS-LRR resistance gene analogs in apricot (Prunus armeniaca L.). Theor. Appl. Genet. 110, 980–989.CrossRefGoogle Scholar
  14. 14.
    Takken, F. L., Albrecht, M., Tameling, W. I. (2006) Resistance proteins: molecular switches of plant defence. Curr. Opin. Plant Biol. 9, 383–390.CrossRefGoogle Scholar
  15. 15.
    Tamura, K., Dudley, J., Nei, M., Kumar, S. (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24, 1596–1599.CrossRefGoogle Scholar
  16. 16.
    Xiao, S. (2006) Current perspectives on molecular mechanisms of plant disease resistance. In: Teixeira da Silva, J. A. (ed.) Floriculture, Ornamental and Plant Biotechnology. Global Science Books, Isleworth, pp. 317–333.Google Scholar
  17. 17.
    Xu, Q., Wen, X., Deng, X. (2007) Phylogenetic and evolutionary analysis of NBS-encoding genes in Rosaceae fruit crops. Mol. Phylogenet. Evol. 44, 315–324.CrossRefGoogle Scholar
  18. 18.
    Yang, S., Zhang, X., Yue, J., Tian, D., Chen, J. (2008) Recent duplications dominate NBS-encoding gene expansion in two woody species. Mol. Genet. Genomics 280, 187–198.CrossRefGoogle Scholar

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© Akadémiai Kiadó, Budapest 2011

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Á. Gutermuth
    • 1
    Email author
  • Zsuzsanna György
    • 1
  • A. Hegedűs
    • 1
  • A. Pedryc
    • 1
  1. 1.Department of Genetics and Plant BreedingCorvinus University of BudapestBudapestHungary

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