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Acta Biologica Hungarica

, Volume 62, Issue 2, pp 171–181 | Cite as

Non-TIR-NBS-LRR Resistance Gene Analogs in Apricot (Prunus Armeniaca L.)

  • Á. GutermuthEmail author
  • Zsuzsanna György
  • A. Hegedűs
  • A. Pedryc
Article

Abstract

Genes encoding for proteins with nucleotide-binding site and leucine-rich repeat motifs (NBS-LRR) have been suggested to play a general role in plant defence mechanism. In Prunus species, many TIR (Toll / Interleukin-1 Receptor), and only very few non-TIR sequences were identified, which was explained either by the unequal distribution of TIR/non-TIR sequences in the Prunus genome or by the incapability of primers in the amplification of non-TIR RGAs. The objective of this work was to check whether a new semi-nested PCR strategy can be developed for the targeted isolation of non-TIR-NBS-LRR Resistance Gene Analog (RGA) sequences from apricot. Three primers (CUB-P-loop F, CUB-Kin2 F and CUB-HD R) were designed, from which CUB-Kin2 F and CUB-HD R were constructed to anneal selectively to the non-TIR sequences. A colony Polymerase Chain Reaction (PCR) indicated that out of the 96 clones tested 28 showed amplification using the newly developed primers, while no amplification occurred when using the formerly described primers. Half of the 28 positive clones were sequenced and they turned out to represent 11 different non-TIR RGA sequences. A phylogenetic analysis was carried out based on an alignment containing 293 Rosaceae and 21 non-Rosaceaa sequences. A significantly higher ratio (91%) of non-TIR sequences were arranged in multi-genera clades than that of (57%) the TIR groups confirming that non-TIR sequences might be of more ancient origin than TIR sequences.

Keywords

Apricot Prunus armeniaca L. resistance gene analogs RGA non-TIR-NBS-LRR 

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The online version of this article (DOI: 10.1556/ABiol.62.2011.2.7) contains supplementary material, which is available to authorized users.

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© Akadémiai Kiadó, Budapest 2011

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Á. Gutermuth
    • 1
    Email author
  • Zsuzsanna György
    • 1
  • A. Hegedűs
    • 1
  • A. Pedryc
    • 1
  1. 1.Department of Genetics and Plant BreedingCorvinus University of BudapestBudapestHungary

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