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Acta Biologica Hungarica

, Volume 62, Issue 1, pp 73–84 | Cite as

Strain-Specific Scar Markers for the Detection of Trichoderma Harzianum AS12-2, a Biological Control Agent Against Rhizoctonia Solani, the Causal Agent of Rice Sheath Blight

  • S. Naeimi
  • S. Kocsubé
  • Zsuzsanna Antal
  • S. M. Okhovvat
  • M. Javan-Nikkhah
  • C. Vágvölgyi
  • L. KredicsEmail author
Article

Abstract

In order to identify a specific marker for T. harzianum AS12-2, a strain capable of controlling rice sheath blight caused by Rhizoctonia solani, UP-PCR was performed using five universal primers (UP) both separately and in pairwise combinations. The application of two UP primers resulted in the amplification of unique fragments from the genomic DNA of T. harzianum AS12-2, clearly distinguishing it from other Trichoderma strains. The unique fragments had no significant sequence homology with any other known sequence available in databases. Based on the sequences of the unique fragments, 14 oligonucleotide primers were designed. Two primer sets amplified a fragment of expected size from the DNA of strain T. harzianum AS12-2 but not from any other examined strains belonging to T. harzianum, to other Trichoderma species assayed, or to other common fungi present in paddy fields of Mazandaran province, Iran. In conclusion, SCAR (sequence characterized amplified regions) markers were successfully identified and rapid, reliable tools were provided for the detection of an effective biocontrol Trichoderma strain, which can facilitate studies of its population dynamics and establishment after release into the natural environment.

Keywords

Rhizoctonia solani Trichoderma harzianum biological control rice sheath blight SCAR markers 

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© Akadémiai Kiadó, Budapest 2011

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • S. Naeimi
    • 1
  • S. Kocsubé
    • 2
  • Zsuzsanna Antal
    • 2
    • 3
  • S. M. Okhovvat
    • 1
  • M. Javan-Nikkhah
    • 1
  • C. Vágvölgyi
    • 2
  • L. Kredics
    • 2
    Email author
  1. 1.Department of Plant Protection, Faculty of AgricultureUniversity of TehranKarajIran
  2. 2.Department of Microbiology, Faculty of Science and InformaticsUniversity of SzegedSzegedHungary
  3. 3.Laboratoire de génomique fonctionnelle des champignons pathogènes des plantesUniversité Claude BernardLyonFrance

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