Advertisement

Acta Biologica Hungarica

, Volume 62, Issue 1, pp 57–64 | Cite as

Effect of Cotton Bollworm (Helicoverpa Armigera Hübner) Caused Injury on Maize Grain Content, Especially Regarding to the Protein Alteration

  • S. KeszthelyiEmail author
  • F. Pál-Fám
  • I. Kerepesi
Article

Abstract

The cotton bollworm (Helicoverpa armigera Hübner), which migrated in the Carpathian-basin from Mediterraneum in the last decades, is becoming an increasingly serious problem for maize producers in Hungary. In several regions the damage it causes has reached the threshold of economic loss, especially in the case of the sweet maize cultivation. The aim of the research was to determine the changing of ears weights and in-kernel accumulation and alteration in grain as a function of cotton bollworm mastication.

Our investigation confirmed that there is an in-kernel and protein pattern change of maize grain by cotton bollworm. Our results proved the significant damaging of each part of ears by cotton bollworm masticating (the average weight loss of ears: 13.99%; the average weight loss of grains: 14.03%; the average weight loss of cobs: 13.74%), with the exception of the increasing of the grain-cob ratio. Our examinations did not prove the water loss–that is the “forced maturing”–caused by the damage. Decreasing of raw fat (control: 2.8%; part-damaged: 2.6%; damaged: 2.4%) and starch content (control: 53.1%; part-damaged: 46.6%; damaged: 44.7%) were registered as a function of injury. In contrast, the raw protein content was increased (control: 4.7%; part-damaged: 5.3%; damaged: 7.4%) by maize ear masticating. The most conspicuous effect on protein composition changing was proved by comparison of damaged grain samples by SDS PAGE. Increased amounts of 114, 50, 46 and 35 kDa molecular mass proteins were detected which explained the more than 50% elevation of raw protein content. The statistical analysis of molecular weights proved the protein realignment as a function of the pest injuries, too.

Keywords

Maize cotton bollworm injury grain content protein pattern 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Blackmer, J. L., Byrne, D. N. (1999) The effect of Bemisia tabaci on amino acid balance in Cucumis melo. Entomol. Exp. Appl. 93, 313–317.CrossRefGoogle Scholar
  2. 2.
    Breiteneder, H., Radauer, C. A. (2004) Classification of plant food allergens. J. Allergy Clin. Immun. 113, 821–830.CrossRefGoogle Scholar
  3. 3.
    Bohn, M., Kreps, R. C., Klein, D., Melchinger, A. E. (1999) Damage and grain yield losses caused by European corn borer (Lepidoptera: Pyralidae) in early maturing European maize hybrids. J. Econ. Entomol. 92, 723–731.CrossRefGoogle Scholar
  4. 4.
    Camprag, D., Baca, F. (1995) Diabrotica virgifera (Coleoptera, Chrysomelidae); A new pest of maize in Yugoslavia. Pestic. Sci. 45, 291–292.CrossRefGoogle Scholar
  5. 5.
    Camprag, D., Sekulic, R., Kereši, T., Baca, F. (2004) Corn Earworm (Helicoverpa armigera Hbn.) and Measures of Integrated Pest Management. Faculty of Agriculture, Novi Sad, p. 183.Google Scholar
  6. 6.
    Csermely, P. (2000) Stress Proteins. Ancient Protection Mechanism of Our Cells. Vince Press, Budapest. [In Hungarian.]Google Scholar
  7. 7.
    Fitt, G. P. (1989) The ecology of Heliothis species in relation to agroecosystems. Annu. Rev. Entomol. 34, 17–52.CrossRefGoogle Scholar
  8. 8.
    Hammon, K. E., Faeth, S. H. (2006) Ecology of plant-herbivore communities: A fungal component? Nat. Toxins 1, 197–208.CrossRefGoogle Scholar
  9. 9.
    Hartl, F. U. (1996) Molecular chaperones in cellular protein folding. Nature 381, 571–580.CrossRefGoogle Scholar
  10. 10.
    Hatcher, P. E., Paul, N. D. (2001) Plant pathogen: herbivore interactions and their effects on weeds. In: Biotic Interactions in Plant-pathogen Associations. CABI Publications, Wallingford, pp. 193–225.CrossRefGoogle Scholar
  11. 11.
    Horváth, Z., Fischl, G. (1996) The appearance of the phytopathogens in sunflower and maize by injuries of the sunflower moth and the cotton bollworm. VI. Keszthelyi Növényvédelmi Fórum, Keszthely (Abstract), 16. [In Hungarian.]Google Scholar
  12. 12.
    Kahler, A. L., Olness, A. E., Sutter, G. R., Dybing, C. D., Devine, O. J. (1985) Root damage by Western corn rootworm and nutrient content in maize. Agron.6 J. 77, 769–774.CrossRefGoogle Scholar
  13. 13.
    Keller, N. P., Bergstrom, G. C., Carruthers, R. I. (1986) Potential yield reductions in maize associated with an anthracnose/European corn borer pest complex in New York. Phytopathology 76, 586–589.CrossRefGoogle Scholar
  14. 14.
    Keszthelyi, S., Takács, A. (2002) Changes of weight and in-kernel content values of maize hybrids (Occitan, Colomba, DK-471) as a result of damaging by European corn borer. J. Cent. Eur. Agric. 3, 169–178.Google Scholar
  15. 15.
    Keszthelyi, S., Szabó, T., Kurucsai, P., Nádasy, M., Marczali, Zs. (2007) Damage determination of Western corn rootworm in soil disinfected, continuous corn. Cer. Res. Commun. 35, 593–596.CrossRefGoogle Scholar
  16. 16.
    Király, L., Barna, B., Király, Z. (2007) A new light upon the plant resistance and its mechanisms. Növénytermelés 56, 65–81.Google Scholar
  17. 17.
    Lodos, N. (1979) Maize pests and their importance in Turkey. Eppo Bull. 11, 87–89.CrossRefGoogle Scholar
  18. 18.
    Ma, X. L., Wang, Z. L., Qi, Y. C., Zhao, Y. X., Zhang, H. (2003) Isolation S-adenosylmethionine synthetase gene from Suadea salsa and its differential expression under NaCl stress. Acta Bot. Sin. 45, 1359–1365.Google Scholar
  19. 19.
    Marton, L. C., Szoke, C., Pintér, J., Bodnár, E. (2009) Studies on the tolerance of maize hybrids to western corn rootworm (Diabrotica virgifera virgifera LeConte). Maydica 54, 217–220.Google Scholar
  20. 20.
    Mesterházy, Á. (1978) Breeding wheat and corn for resistance to Fusarium spp. in seedling stage. 3rd International Congress of Plant Pathology: Munich, 16–23 August 1978. Wageningen, p. 288.Google Scholar
  21. 21.
    Mesterházy, Á., Kovács, K. (1986) Breeding corn against fusarial stalk rot, ear rot and seedling blight. Acta Phytopath. Entomol. Hung. 21, 231–249.Google Scholar
  22. 22.
    Meyers, B. C., Kozik, A., Griego, A., Kuang, H., Michelmore, R. W. (2003) Genome-wide analysis of NBS-LRR-encoding genes in Arabidopsis. Plant Cell 15, 809–834.CrossRefGoogle Scholar
  23. 23.
    Mile, L., Ilovay, Z. (1979) Damage examinations of European corn borer (Ostrinia nubilis Hbn.) in the case of industrial productionaly conditions. Növényvédelem 15, 313–315.Google Scholar
  24. 24.
    Munkvold, G. P., Hellmich, R. L., Showers, W. B. (1997) Reduced fusarium ear rot and symptomless infection in kernels of maize genetically engineered for european corn borer resistance. Phytopathology 87, 1071–1077.CrossRefGoogle Scholar
  25. 25.
    Pál-Fám, F., Kerepesi, I., Keszthelyi, S., Pozsgai, J. (2008) Germination, enzyme activity and nutrient contents of hail stormed corn in the case of corn smut fungus [Ustilago maydis (DC.)Corda]. Cer. Res. Commun. 36, 195–198.CrossRefGoogle Scholar
  26. 26.
    Pálfy, Cs. (1983) The European corn borer and its damage. Növényvédelem 19, 515–517.Google Scholar
  27. 27.
    Ruming, L., Manjit, S. K., Orlando, J. M., Linda, M. P. (2004) Relationship among Aspergillus flavus infection, maize weevil damage, and ear moisture loss in exotic × adapted maize. Cer. Res. Commun. 32, 371–377.Google Scholar
  28. 28.
    Schulze-Leifert, P., Bieri, S. (2005) Recognition at a distance. Science 308, 506–508.CrossRefGoogle Scholar
  29. 29.
    Stone, P. J., Nicolas, M. E. (1998) Comparison of sudden heat stress with gradual exposure to high temperature during grain filling in two wheat varieties differing in heat tolerance. II. Fractional protein accumulation. Aust. J. Plant Physiol. 25, 1–11.Google Scholar
  30. 30.
    Stone, P. J., Nicolas, M. E. (1998) The effect of duration of heat stress during grain filling on two wheat varieties differing in heat tolerance: Grain growth and fractional protein accumulation. Aust J. Plant Physiol. 25, 13–20.Google Scholar
  31. 31.
    Szoke, Cs., Zsubori, Z., Pók, I., Rácz, F., Illés, O., Szegedi, I. (2005) Significance of the European corn borer (Ostrinia nubilalis Hbn.) in maize production. Acta Agron. Hung. 50, 447–461.Google Scholar
  32. 32.
    Szigeti, Z. (1998) Plants and the stress. In: Láng, F. (ed.): Plant Physiology. The Vegetal Metabolism. ELTE, Eötvös Press. [In Hungarian.]Google Scholar
  33. 33.
    Tollefson, J. J. (2007) Evaluating maize for resistance to Diabrotica virgifera virgifera Leconte (Coleoptera: Chrysomelidae). Maydica 52, 311–318.Google Scholar
  34. 34.
    Tóthmérész, B. (1996) NuCoSA. Software for Botanical-, Zoogical- and Ecological Experiments. Scientia Press, Budapest.Google Scholar
  35. 35.
    Waldo, D. R. (1973) Extent and partition of cereal grain starch digestion in ruminants. J. Anim. Sci. 37, 1062–1074.CrossRefGoogle Scholar
  36. 36.
    Wilhelm, E. P., Mullen, R. E., Keeling, P. L., Singletary, G. W. (1999) Heat stress during grain filling in maize: Effects on kernel growth and metabolism. Crop Sci. 39, 1733–1741.CrossRefGoogle Scholar
  37. 37.
    Wilson, C. M. (1991) Multiple zeins from maize endosperms characterized by reverse-phase high performance liquid chromatography. Plant Physiol. 95, 777–786.CrossRefGoogle Scholar
  38. 38.
    Woo, Y., Hu, M. D., Larkins, B., Jung, R. (2001) Genomic analysis of genes expressed in maize endosperm identifies novel seed proteins and clarifies patterns of zein gene expression. Plant Cell 13, 2297–2317.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2011

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Department of Botany and Plant Production, Faculty of Animal ScienceUniversity of KaposvárKaposvárHungary
  2. 2.Department of Genetics and Molecular Biology, Faculty of ScienceUniversity of PécsPécsHungary

Personalised recommendations