Effect of In Vitro Culture Conditions on Somaclonal Variation in Cowpea (Vigna Unguiculata Walp.) Using Rapd Markers

Abstract

We report a high frequency regeneration protocol in cowpea (Vigna unguiculata Walp. var. C 152) via somatic embryogenesis from 10-d-old primary leaf explants. A study was conducted to examine the effect of somaclonal variations in in vitro derived cowpea plants under field conditions. The regenerated plantlets were successfully transferred to field after hardening in vitro and grown for collecting R0, R1 and R2 seeds. The seeds of R1 and R2 generations were subsequently, grown under field conditions and their various biometrical traits were compared and evaluated with non-tissue cultured cowpea plants as check. There was no detectable somaclonal variation induced in R0–R2 in any of the biometrical traits. The results indicate that the inclusion of different plant growth promoters at specified concentrations and duration in our earlier tissue culture work did not induce any detectable mutation. The RAPD analysis also shows that there is no genetic variation among R2 cowpea plants. The somatic embryogenesis protocol we report could thus be safely applied for high frequency true-to-type regeneration and transformations protocols without any somaclonal variation.

Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

BAP:

6-benzylamino purine

CH:

casein hydrolysate

Gln:

L-Glutamic acid-5-amide

2ip:

isopentenyl adenine

KIN:

Kinetin

MS:

Murashige & Skoog’s medium

MMS:

MS salts with B5 vitamins

Pro:

L-proline

TDZ:

thidiazuron

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Correspondence to P. Sivakumar.

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Sivakumar, P., Rajesh, S., Gnanam, R. et al. Effect of In Vitro Culture Conditions on Somaclonal Variation in Cowpea (Vigna Unguiculata Walp.) Using Rapd Markers. BIOLOGIA FUTURA 62, 34–44 (2011). https://doi.org/10.1556/ABiol.61.2011.1.3

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Keywords

  • Cowpea
  • plant growth regulators
  • somaclonal variation