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Acta Biologica Hungarica

, Volume 61, Issue 4, pp 498–511 | Cite as

Influence of Genotype and Explant Source on the In Vitro Regeneration Ability of Different Melon Varieties

  • Erzsébet Kiss-Bâba
  • Sarolta Pânczél
  • I. Velich
  • G. D. BisztrayEmail author
Article

Abstract

Nine genotypes of melon (Cucumis melo L.) were selected for the investigation of regeneration. Most of the tested varieties showed regeneration ability on medium containing 0.5 mg 1−1 or 1 mg 1−1 BA, but following the appearance of shoot buds, only six varieties produced leafy shoots. The effect of combinations of BA with different auxins (IAA, NAA, 2,4-D) and ABA in the culture medium on shoot regeneration was tested on cotyledon expiants of ‘Hógolyó’ and ’Hale’s Best’. To establish optimal conditions for the adventitious shoot induction six types of seedling-derived expiants were prepared from seedlings of four different ages. The best results for shoot forming capacity were achieved with cotyledons followed by decapitated seedlings and hypocotyls derived from 4-day-old seedlings. Cotyledon segments of ‘Hógolyó’ and ‘Hale’s Best’ were also cultivated on media with different concentrations of IAA and BA supplemented with 0.26 mg 1−1 ABA. The highest number of well-formed plantlets was counted for ‘Hógolyó’ on the medium supplemented with 0.9 mg 1−1 BA+0.6 mg 1−1 IAA+0.26 mg 1−1 ABA. This is the first report on the in vitro regeneration of ’Hógolyó’ from decapitated seedling and hypocotyl expiants and of ‘Javitott Zentai’, ‘Muskotály’, ‘Hógolyó’, ‘Tétényi csereshéjú’ and ‘Magyar Kincs’ from cotyledon expiants.

Keywords

Melon in vitro regeneration cotyledon decapitated seedlings 

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Notes

Acknowledgements

The authors would like to thank Barbara Harasztos for the critical reading and revising of the manuscript. Thanks are also due to Agnes Gyurcsa-Millei for technical assistance, and to Oliver Kiss for his help in the statistical analysis.

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© Akadémiai Kiadó, Budapest 2010

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Erzsébet Kiss-Bâba
    • 1
  • Sarolta Pânczél
    • 1
    • 2
  • I. Velich
    • 1
  • G. D. Bisztray
    • 1
    Email author
  1. 1.Department of Genetics and Plant Breeding, Faculty of Horticultural ScienceCorvinus University of BudapestBudapestHungary
  2. 2.Department of Applied GenomicsAgricultural Research Institute of the Hungarian Academy of SciencesMartonvâsârHungary

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