Xylopia aethiopica (XA) (Annonaceae) possesses great nutritional and medicinal values. This study was designed to investigate the effects of XA fruit methanol extract on oxidative stress in brain of rats exposed to whole body γ-radiation (5 Gy). Vitamin C (VC) served as standard antioxidant. Forty-four rats were divided into 4 groups of 11 rats each. One group served as control, two different groups were treated with XA and VC (250 mg/kg), 6 weeks before and 8 weeks after irradiation, and fourth group was only irradiated. Rats were sacrificed 1 and 8 weeks after irradiation. The antioxidant status, viz. Lipid peroxidation (LPO), superoxide dismutase (SOD), catalase (CAT), glutathione-s-transferase (GST) and glutathione (GSH) were estimated. Results indicate a significant increase (p < 0.05) in levels of brain LPO after irradiation. LPO increased by 90% and 151%, after 1 and 8 weeks of irradiation, respectively. Irradiation caused significant (p < 0.05) decreases in levels of GSH and GST by 61% and 43% after 1 week and, 75% and 73%, respectively, after 8 weeks of exposure. CAT and SOD levels were decreased by 62% and 68%, respectively, after 8 weeks of irradiation. Treatment with XA and VC ameliorated the radiation-induced decreases in antioxidant status of the animals. These suggest that XA could have beneficial effect by inhibiting oxidative damage in brain of exposed rats.
Adaramoye, O., Ogungbenro, B., Anyaegbu, O., Fafunso, M. (2008) Protective effects of extracts of Vernonia amygdalina, Hibiscus sabdariffa and vitamin C against radiation-induced liver damage in rats. J. Radiat. Res. 49, 123–131.
Aebi, H. (1974) Methods of Enzymatic Analysis. In: Bergmeyer, H. V. (ed.) Catalase estimation. Verlag Chemic, New York, pp. 673–684.
Anuradha, C. V., Selvam, R. (1993) Effect of oral methionine on tissue lipid peroxidation and antioxidants in alloxan-induced diabetic rats. J. Nutr. Biochem. 4, 212–217.
Baynes, J. W., Thrope S. R. (1999) Oxidative stress in diabetic complications. Diabetes 48, 1–9.
Bolzan, A. D., Bianchi M. S. (2002) Genotoxicity of STZ. Mutat. Res. 512, 121–134.
Breen, A. P., Murphy J. A. (1995) Reactions of oxyl radicals with DNA. Free Radic. Biol. Med. 18, 1033–1077.
Buege, J. A., Aust S. D. (1978) Microsomal lipid peroxidation. Methods Enzymol. 30, 302–310.
Carney, J. M., Strake-Reed, P. E., Oliver, C. N., Landum, R. W., Chang, M. S., Wu, J. F., Floyd R. A. (1991) Reversal of age-related increase in brain protein oxidation, decrease in enzyme activity and loss in temporal and spatial memory by chronic administration of the spin trapping compound N-tert-buty-alfa-pheynitrone. PNAS 88, 3633–3636.
Cerutti, P. A. (1991) Oxidant stress and carcinogenesis. Eur. J. Clin. Invest. 21, 1–5.
Choi, J. H., Yu B. P. (1995) Brain synaptosomal aging: Free radicals and membrane fluidity. Free Radic. Biol. Med 18, 133–139.
Dest, V. M. (2006) Radioprotectants: adding quality of life to survivorship. Semin. Oncol. Nurs. 22, 249–256.
Diderot, N. T., Silvere, N., Yasin, A., Zareen, S., Fabien, Z., Etienne, T., Choudhary, M. I., Atta-Ur, R. (2005) Prolyl endopeptidase and thrombin inhibitory diterpenoids from the bark of Xylopia aethi-opica. Biosci. Biotechnol. Biochem. 69, 1763–1766.
Esterbauer, H. (1996) Estimation of peroxidative damage: A critical review. Pathologie Biologie Paris 44, 25–28.
Finkel, T., Holbrook N. J. (2000) Oxidants, oxidative stress and the biology of ageing. Nature 408, 239–247.
Habig, W. H., Pabst, M. J., Jakoby W. B. (1974) Glutathione-S-transferases. The first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249, 7130–7139.
HariKumar, K. B., Kuttan, R. (2004) Protective effect of an extract of Phyllanthus amarus against radiation-induced damage in mice. J. Radiat. Res. 45, 133–139.
Hosseinimehr, S. J. (2007) Foundation review: trends in the development of radioprotective agents. Drug Discov. Today 12, 794–805.
Jagetia, G. C., Venkatesh, P., Baliga M. S. (2004) Fruit extract of Aegle marmelos protects mice against radiation-induced lethality. Integr. Cancer Ther 3, 323–332.
Kada, T., Kaneko, K., Matsuzaki, S., Matsuzaki, T., Hara, Y. (1985) Detection and chemical identification of natural bio-antimutagens. A case of the green tea factor. Mutat. Res. 150, 127–132.
Krishna, A., Kumar, A. (2005) Evaluation of radioprotective effects of Rajgira (Amaranthus paniculatus) extract in Swiss albino mice. J. Radiat. Res. 46, 233–239.
Lowry, O. H., Rosenbrough, N J., Farr, A. L., Randall R. J. (1951) Protein measurement with Folin phenol reagent. J. Biol. Chem. 193, 265–275.
Lubec, B., Hayn, M., Denk W., Bauer, G. (1996) Brain lipid peroxidation and hydroxyl radical attack following the intravenous infusion of hydrogen peroxide in an infant. Free Radic. Biol. Med. 21, 219–223.
Manikandan, S., Srikumar, R., Parthasarathy, N. J., Devi R. S. (2005) Protective effect of Acorus calamus LINN on free radical scavengers and lipid peroxidation in discrete regions of brain against noise stress exposed rat. Biol. Pharm. Bull. 28, 2327–2330.
Matcovis, B., Varga, S. L., Szaluo, L., Witsas, H. (1982) The effect of diabetes on the activities of the peroxide metabolic enzymes. Hor. Metab. Res. 14, 77–79.
McCord, J. M., Fridovich, I. (1969) Superoxide dismutase, an enzymatic function for erythrocuperin. J. Biol. Chem. 244, 6049–6055.
Moron, M. A., Depierre, J. W., Mannervick, B. (1979) Levels of glutathione, glutathione reductase and glutathione-S-transferase activities in rat lung and liver. Bioch. Biophy. Acta 582, 67–78.
Ngouela, S., Nyassé, B., Tsamo, E., Brochier, M. C., Morin, C. (1998) A trachylobane diterpenoid from Xylopia aethiopica. J. Nat. Prod. 61, 264–266.
O’Neill, P., Fieden E. M. (1993) DNA: Advances in Radiation Biology. Academic Press, New York pp. 53–120.
Packer, L. S., Ong A. S. H. (1998) Molecular Mechanisms and Health Effects. AOCS Press, Champaign, IL, pp. 307.
Packer, L., Tritschler, H. J., Wessel, K. (1997) Neuroprotection by the metabolic antioxidant alpha-lipoic acid. Free Radic. Biol. Med. 22, 359–378.
Slyshenkov, V. S., Moiseenok, A. G., Wojtczak, L. (1996) Noxious effects of oxygen reactive species on energy-coupling processes in Ehrlich ascites tumor mitochondria and the protection by pantothenic acid. Free Radic. Biol. Med. 20, 793–800.
UNSCEAR (1986) United Nations Scientific Committee on the Effects of Atomic Radiation, Genetic and Somatic Effects of Ionizing Radiation, 1986 report to the General Assembly, with annexes. New York, United Nations.
Van Campon, L. E., Murphy, W. J., Franks, J. R., Maethias, P. I., Toraaason M. A. (2002) Oxidative DNA damage is associated with intensive noise exposure in the rat. Hear. Res. 164, 29–38.
Yu, B. P. (1994) Cellular defense against damage from reactive oxygen species. Physiol. Rev. 74, 139–162.
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Adaramoye, O.A., Popoola, B.O. & Farombi, E.O. Effects of xylopia Aethiopica (Annonaceae) Fruit Methanol Extract on γ-Radiation-Induced Oxidative Stress in Brain of Adult Male Wistar Rats. BIOLOGIA FUTURA 61, 250–261 (2010). https://doi.org/10.1556/ABiol.61.2010.3.2
- Antioxidant enzymes
- oxidative stress
- vitamin C