Towards Reducing Genotype Specificity in Regeneration Protocols After Somatic Hybridization Between Cultivated Sunflower and Wild Helianthus Species

Abstract

Successful application of somatic hybridization in sunflower breeding is limited by low regeneration percentage from fusion products. The effect of pre-treatment media composition on shoot regeneration from electro fusion products of wild and cultivated sunflower was investigated in this paper, with the aim to determine if there is room for improvement of the existing regeneration protocols in order to make them less genotype-dependent. Protoplasts isolated from hypocotyls of two inbred lines of cultivated sunflower were electrofused with mesophyll protoplasts of H. mollis and H. maximiliani. The regeneration was induced only on calluses that were grown on the media with low auxin and high cytokinin contents during the development of calluses from microcalluses (pre-treatment), with silver nitrate as a medium component. Regeneration from fusion products was induced by treatment with a high concentration of TDZ or 2,4 D for a short period of time.

Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

BAP:

6-benzylamino-purine

GA3:

gibberellic acid

IBA:

indolebuteric acid

NAA:

naphthaleneacetic acid

PCR:

polymerase chain reaction

RAPD:

random amplified polymorphic DNA

TDZ:

thidiazuron

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Acknowledgements

This work was supported by the Serbian Ministry of Science and Technological Development.

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Correspondence to Ksenija Taski-Ajdukovic.

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Taski-Ajdukovic, K., Nagl, N. & Miladinovic, D. Towards Reducing Genotype Specificity in Regeneration Protocols After Somatic Hybridization Between Cultivated Sunflower and Wild Helianthus Species. BIOLOGIA FUTURA 61, 214–223 (2010). https://doi.org/10.1556/ABiol.61.2010.2.9

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Keywords

  • Cytokinin
  • electrofusion
  • Helianthus
  • protoplasts
  • RAPD
  • regeneration