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Acta Biologica Hungarica

, Volume 61, Issue 2, pp 214–223 | Cite as

Towards Reducing Genotype Specificity in Regeneration Protocols After Somatic Hybridization Between Cultivated Sunflower and Wild Helianthus Species

  • Ksenija Taski-AjdukovicEmail author
  • Nevena Nagl
  • Dragana Miladinovic
Article

Abstract

Successful application of somatic hybridization in sunflower breeding is limited by low regeneration percentage from fusion products. The effect of pre-treatment media composition on shoot regeneration from electro fusion products of wild and cultivated sunflower was investigated in this paper, with the aim to determine if there is room for improvement of the existing regeneration protocols in order to make them less genotype-dependent. Protoplasts isolated from hypocotyls of two inbred lines of cultivated sunflower were electrofused with mesophyll protoplasts of H. mollis and H. maximiliani. The regeneration was induced only on calluses that were grown on the media with low auxin and high cytokinin contents during the development of calluses from microcalluses (pre-treatment), with silver nitrate as a medium component. Regeneration from fusion products was induced by treatment with a high concentration of TDZ or 2,4 D for a short period of time.

Keywords

Cytokinin electrofusion Helianthus protoplasts RAPD regeneration 

Abbreviations

2,4-D

2,4-dichlorophenoxyacetic acid

BAP

6-benzylamino-purine

GA3

gibberellic acid

IBA

indolebuteric acid

NAA

naphthaleneacetic acid

PCR

polymerase chain reaction

RAPD

random amplified polymorphic DNA

TDZ

thidiazuron

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Notes

Acknowledgements

This work was supported by the Serbian Ministry of Science and Technological Development.

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© Akadémiai Kiadó, Budapest 2010

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Ksenija Taski-Ajdukovic
    • 1
    Email author
  • Nevena Nagl
    • 1
  • Dragana Miladinovic
    • 1
  1. 1.Institute of Field and Vegetable CropsNovi SadSerbia

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