Towards Reducing Genotype Specificity in Regeneration Protocols After Somatic Hybridization Between Cultivated Sunflower and Wild Helianthus Species
Successful application of somatic hybridization in sunflower breeding is limited by low regeneration percentage from fusion products. The effect of pre-treatment media composition on shoot regeneration from electro fusion products of wild and cultivated sunflower was investigated in this paper, with the aim to determine if there is room for improvement of the existing regeneration protocols in order to make them less genotype-dependent. Protoplasts isolated from hypocotyls of two inbred lines of cultivated sunflower were electrofused with mesophyll protoplasts of H. mollis and H. maximiliani. The regeneration was induced only on calluses that were grown on the media with low auxin and high cytokinin contents during the development of calluses from microcalluses (pre-treatment), with silver nitrate as a medium component. Regeneration from fusion products was induced by treatment with a high concentration of TDZ or 2,4 D for a short period of time.
KeywordsCytokinin electrofusion Helianthus protoplasts RAPD regeneration
polymerase chain reaction
random amplified polymorphic DNA
Unable to display preview. Download preview PDF.
This work was supported by the Serbian Ministry of Science and Technological Development.
- 1.Aslane-Chanabe, C. (1991) Regeneration de plantes a partir de protoplastes chez le genre Helianthus et hybridation somatique entre le tournesol cultive et les tournesols sauvages. Ph.D. TNP-ENSAT, Toulouse, France.Google Scholar
- 6.Chraibi, K., Latche, A., Roustan, J., Fallot, J. (1991) Stimulation of shoot regeneration from cotyledons of Helianthus annuus by ethylene inhibitors, silver and cobalt. Plant Cell Rep. 10, 204–207.Google Scholar
- 13.Henn, H. J., Steiner, R., Wingender, R., Schnabl, H. (1997) Wild type sunflower clones: Source of resistance against Sclerotinia sclerotiorum (Lib) de Bary stem infection. Angew. Bot. 71, 5–9.Google Scholar
- 19.Paques, M., Boxus, P. (1987) Vitrification: a phenomenon related to tissue water content. ActaHort 212, 245–252.Google Scholar
- 26.Thorpe, T. A. (1978) Physiological and biochemical aspects of organogenesis in vitro. In: Thorpe, T. A. (ed.) Frontiers of Plant Tissue Culture. International Association for Plant Tissue Culture, Calgary pp. 49–58.Google Scholar
- 27.Trabace, T., Vischi, M., Fiore, M. C., Sunseri, F., Vanadia, S., Marchetti, S., Olivieri, A. M. (1995) Plant regeneration from hypocotyl protoplast in sunflower (Helianthus annuus L). J. Genet. Breed 49, 51–54.Google Scholar
- 28.Trabace, T., Fiore, M. C., D’Ambrosio, C., Vanadia, S., Sunseri, F. (1996) Sunflower cytoplasmic hybrids relevant by PCR assay using male sterility as selectable marker. J. Genet. Breed. 50, 29–34.Google Scholar
- 31.Vasic, D. (2003) Sunflower Somatic Hybridization. Andrejevic Endowment, Belgrade, SerbiaGoogle Scholar
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.