Hepatocurative Potential of Sesquiterpene Lactones of Taraxacum officinale on Carbon Tetrachloride Induced Liver Toxicity in Mice
The hepatocurative potential of ethanolic extract (ETO) and sesquiterpene lactones enriched fraction (SL) of Taraxacum officinale roots was evaluated against carbon tetrachloride (CCl4) induced hepatotoxicity in mice. The diagnostic markers such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and total bilirubin contents were significantly elevated, whereas significant reduction in the level of reduced glutathione (GSH) and enhanced hepatic lipid peroxidation, liver weight and liver protein were observed in CCl4 induced hepatotoxicity in mice. Post-treatment with ETO and SL significantly protected the hepatotoxicity as evident from the lower levels of hepatic enzyme markers, such as serum transaminase (ALT, AST), ALP and total bilirubin. Further, significant reduction in the liver weight and liver protein in drug-treated hepatotoxic mice and also reduced oxidative stress by increasing reduced glutathione content and decreasing lipid peroxidation level has been noticed. The histopathological evaluation of the liver also revealed that ETO and SL reduced the incidence of liver lesions induced by CC14. The results indicate that sesquiterpene lactones have a protective effect against acute hepatotoxicity induced by the administration of CC14 in mice. Furthermore, observed activity of SL may be due to the synergistic action of two sesquiterpene lactones identified from enriched ethyl acetate fraction by HPLC method.
KeywordsCompositae Taraxacum officinale carbon tetrachloride hepatotoxicity sesquiterpene lactones
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Authors would like to thank Dr. S. Soosai Raj for plant identification and Dr. G. Mathan for helpful discussion and critical reading of the manuscript.
- 5.Bown, D. (1995) Encyclopedia of Herbs and Their Uses. Dorling Kindersley, London, pp. 20–31.Google Scholar
- 6.Chiej, R. (1984) Encyclopedia of Medicinal Plants. Mac Donald, USA.Google Scholar
- 11.Foster, S., Duke, J. A. (1990) A Field Guide to Medicinal Plants of Eastern and Central North America. Houghton Mifflin Co., Boston.Google Scholar
- 13.Gueeri, H. (1995) Influence on prolonged ethanol intake on the level and turnover of alcohol and aldehyde dehydrogenase and glutathione. Adv. Exp. Med. Biol. 23, 133–134.Google Scholar
- 14.Harper, H. A. (1961) The Functions and Tests of the Liver, Review of Physiological Chemistry. Lange Medical Publishers, Los Atlos, CA, pp. 271–283.Google Scholar
- 21.Launert, E. (1981) Edible and Medicinal Plants. Hamlyn, UK.Google Scholar
- 24.Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J. (1951) Protein measurement with folin phenol reagent. J. Biol. Chem. 193, 265–275.Google Scholar
- 25.Mahesh, A., Jeyachandran, R., Cindrella, L., Thangadurai, D., Velayutham, R., Pinheiro de Carvalho, M. A. A. (2008) In: Thangadurai, D., Tang, W., Ramachandran, A., Moraes, I. O., Pinheiro de Carvalho M. A. A. (eds) Biotechnology for Food Environment and Agriculture. Agrobios (India), Jodhpur, pp. 235–251.Google Scholar
- 28.Molander, D. W., Wroblewsk, F., La Due, J. S. (1955) Transaminase compared with cholinesterase and alkaline phosphatase as an index of hepatocellular integrity. Clin. Res. Proc. 3, 20–24.Google Scholar
- 36.Recknagel, R. O., Glende Jr, E. A., Britton, R. S. (1991) Free radical damage and lipid peroxidation. In: Meeks, R. G. (eds) Hepatotoxicology. CRC Press, Florida, pp. 401–436.Google Scholar
- 39.Siyuan, Z., Yen-Kim, W., Choon-Nam, O., Han-Ming, S. (2005) Anti-cancer potential of sesquiterpene lactones: Bioactivity and molecular mechanisms. Current Medicinal Chemistry 5, 239–249.Google Scholar
- 45.Zimmerman, H. J., Seef L. B. (1970) Enzymes in hepatic disease. In: Goodley, E. L. (ed.), Diagnostic Enzymology. Philadelphia, pp. 1–38.Google Scholar
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