Photosystem II Functionality and Antioxidant System Changes During Leaf Rolling in Post-Stress Emerging Ctenanthe setosa Exposed to Drought

Abstract

We studied the changes in antioxidant system and chlorophyll fluorescence parameters in post-stress emerging Ctenanthe setosa (Rosc.) Eichler (Marantaceae) plants (PSE plants) having reduced leaf area under drought stress causing leaf rolling and re-watering. PSE plants were compared to primary stressed plants (PS) in previous studies. The parameters were measured at different visual leaf rolling scores from 1 to 4 (1 is unrolled, 4 is tightly rolled and the others is intermediate form). Water potentials and stomatal conductance of leaves were gradually decreased during leaf rolling. Similarly, maximum quantum efficiency of open PS II center and quantum yield of PS II decreased during the rolling period. Non-photochemical quenching of chlorophyll fluorescence decreased at score 2 then increased while photochemical quenching did not change during leaf rolling. Electron transport rate decreased only at score 4 but approximately reached to score 1 level after re-watering. Superoxide dismutase activity was not constant at all leaf rolling scores. Ascorbate peroxidase, catalase and glutathione reductase activities generally tended to increase during leaf rolling. Lipid peroxidation and H2O2 content increased at score 2 but decreased at the later scores. On the other hand, O2·− production increased during the rolling period. After re-watering of the plants having score 4 of leaf rolling, antioxidant enzyme activities were lower than those of score 1. Other physiological parameters also tended to reach the value of score 1. The results indicated that PSE plants gained drought tolerance by reducing leaf area effectively induced their antioxidant systems and protected the photosynthesis under drought stress similar to PS plants.

Abbreviations

APX:

Ascorbate peroxidase

Car:

carotenoid

Chl:

chlorophyll

CAT:

catalase

F m :

maximum fluorescence yield

F 0 :

minimum fluorescence yield

F s :

steady-state fluorescence yield

Fv/Fm:

quantum efficiency of photosystem II

NPQ :

non-photochemical quenching of chlorophyll fluorescence

PSII:

photosystem II

qP :

photochemical quenching

GR:

glutathione reductase

RW:

Re-watering

RWC:

relative water content

gs:

stomatal conductance

SOD:

superoxide dismutase

Ψleaf:

leaf water potential

ΦPS II:

quantum yield of PSII

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Correspondence to Neslihan Saruhan.

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Terzi, R., Saruhan, N., Sağam, A. et al. Photosystem II Functionality and Antioxidant System Changes During Leaf Rolling in Post-Stress Emerging Ctenanthe setosa Exposed to Drought. BIOLOGIA FUTURA 60, 417–431 (2009). https://doi.org/10.1556/ABiol.60.2009.4.8

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Keywords

  • Antioxidant enzymes
  • Ctenanthe setosa
  • chlorophyll fluorescence
  • leaf rolling
  • post-stress emerging plant