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Photosystem II Functionality and Antioxidant System Changes During Leaf Rolling in Post-Stress Emerging Ctenanthe setosa Exposed to Drought

Acta Biologica Hungarica volume 60, pages 417–431 (2009)Cite this article

Abstract

We studied the changes in antioxidant system and chlorophyll fluorescence parameters in post-stress emerging Ctenanthe setosa (Rosc.) Eichler (Marantaceae) plants (PSE plants) having reduced leaf area under drought stress causing leaf rolling and re-watering. PSE plants were compared to primary stressed plants (PS) in previous studies. The parameters were measured at different visual leaf rolling scores from 1 to 4 (1 is unrolled, 4 is tightly rolled and the others is intermediate form). Water potentials and stomatal conductance of leaves were gradually decreased during leaf rolling. Similarly, maximum quantum efficiency of open PS II center and quantum yield of PS II decreased during the rolling period. Non-photochemical quenching of chlorophyll fluorescence decreased at score 2 then increased while photochemical quenching did not change during leaf rolling. Electron transport rate decreased only at score 4 but approximately reached to score 1 level after re-watering. Superoxide dismutase activity was not constant at all leaf rolling scores. Ascorbate peroxidase, catalase and glutathione reductase activities generally tended to increase during leaf rolling. Lipid peroxidation and H2O2 content increased at score 2 but decreased at the later scores. On the other hand, O2·− production increased during the rolling period. After re-watering of the plants having score 4 of leaf rolling, antioxidant enzyme activities were lower than those of score 1. Other physiological parameters also tended to reach the value of score 1. The results indicated that PSE plants gained drought tolerance by reducing leaf area effectively induced their antioxidant systems and protected the photosynthesis under drought stress similar to PS plants.

Abbreviations

APX:

Ascorbate peroxidase

Car:

carotenoid

Chl:

chlorophyll

CAT:

catalase

F m :

maximum fluorescence yield

F 0 :

minimum fluorescence yield

F s :

steady-state fluorescence yield

Fv/Fm:

quantum efficiency of photosystem II

NPQ :

non-photochemical quenching of chlorophyll fluorescence

PSII:

photosystem II

qP :

photochemical quenching

GR:

glutathione reductase

RW:

Re-watering

RWC:

relative water content

gs:

stomatal conductance

SOD:

superoxide dismutase

Ψleaf:

leaf water potential

ΦPS II:

quantum yield of PSII

References

  1. 1.

    Aebi, H. E. (1983) Catalase. In: Bergmeyer H.U. (ed.) Methods of Enzymatic Analysis. Verlag Chemie, Weinhern., pp. 273–286.

    Google Scholar 

  2. 2.

    Aroca, R., Irigoyen, J. J., Sanchez-Diaz, M. (2003) Drought enhances maize chilling toleranceg. II. Photosynthetic traits and protective mechanism against oxidative stress. Physiol. Plant. 117, 540–549.

    CAS  Article  Google Scholar 

  3. 3.

    Arnon, D. I. (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 24, 1–15.

    CAS  Article  Google Scholar 

  4. 4.

    Beauchamp, C., Fridovich, I. (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44, 276–287.

    CAS  Article  Google Scholar 

  5. 5.

    Biehler, K., Haupt, S., Beckmann, J., Fock, H., Becker, T. W. (1997) Simultaneous CO2 and 16O2: 18O2-gas exchange and fluorescence measurements indicate differences in light energy dissipation between the wild-type and the phytochrome-deficient aurea mutant of tomato during water stress. J. Exp. Bot. 48, 1439–1449.

    CAS  Article  Google Scholar 

  6. 6.

    Bilger, W., Björkman, O. (1990) Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absorbance changes, fluorescence and photosynthesis in leaves of Hedera canariensis. Photosynth. Res. 25, 173–185.

    CAS  Article  Google Scholar 

  7. 7.

    Blum, A. (1996) Crop responses of drought and the interpretation of adaptation. Plant Growth Regul. 20, 135–148.

    CAS  Article  Google Scholar 

  8. 8.

    Bowler, C., van Montagu, M., Inzé, D. (1992) Superoxide dismutase and stress tolerance. Annu. Rev. Plant Phys. 43, 83–116.

    CAS  Article  Google Scholar 

  9. 9.

    Butow, B., Wynne, D., Sukenik, A., Hadas, O., Tel-Or, E. (1998) The synergistic effect of carbon concentration and high temperature on lipid peroxidation in Peridinium gatunense. J. Plankton Res. 20, 355–369.

    CAS  Article  Google Scholar 

  10. 10.

    Capaldi, D. J., Taylor, K. E. (1983) A new peroxidase color reaction: oxidative coupling of 3-methyl-2-benzothiazolinone hydrazone (MBTH) with its formaldehyde azine. Application to glucose and choline oxidases. Anal. Biochem. 129, 329–336.

    CAS  Article  Google Scholar 

  11. 11.

    Cornic, G., le Gouallec, J. L., Briantais, J. M., Hodges, M. (1989) Effect of dehydration and high light on photosynthesis of two C3 plants (Phaseolus vulgaris L. and Elatostema repens (Lour) Hall f.). Planta. 177, 84–90.

    CAS  Article  Google Scholar 

  12. 12.

    Dall’Osto, L., Fiore, A., Cazzaniga, S., Giuliano, G., Bassi, R. (2007) Different roles of α- and β-branch xanthophylls in photosystem assembly and photoprotection. J. Biol. Chem. 282, 35056–36068.

    Article  Google Scholar 

  13. 13.

    Foyer, C. H., Halliwell, B. (1976) Presence of glutathione and glutathione reductase in chloroplast: a proposed role in ascorbic acid metabolism. Planta 133, 21–25.

    CAS  Article  Google Scholar 

  14. 14.

    Foyer, C. H., Descourvieres, P., Kunert, K. J. (1994) Protection against oxygen radicals: an importance defence mechanism studied in transgenic plants. Plant Cell Environ. 17, 507–523.

    CAS  Article  Google Scholar 

  15. 15.

    Fracheboud, Y., Leipner, J. (2003) The application of chlorophyll fluorescence to study light, temperature, and drought stress. In: DeEll, J. R., Toivonen, P. M. A. (eds) Practical Applications of Chloropyll Fluorescence in Plant Biology. Kluwer Acad. Pub., USA, pp. 126–147.

    Google Scholar 

  16. 16.

    Frahry, G., Schopfer, P. (2001) NADH-stimulated, cyanide-resistant superoxide production in maize coleoptiles analyzed with a tetrazolium-based assay. Planta 212, 175–183.

    CAS  Google Scholar 

  17. 17.

    Gamble, P. E., Burke, J. J. (1984) Effect of water stress on the chloroplast antioxidant system I. Alterations in glutathione reductase activity. Plant Physiol. 76, 615–621.

    CAS  Article  Google Scholar 

  18. 18.

    Halliwell, B., Gutteridge, J. N. C. (1999) Free Radicals in Biology and Medicine. 3rd ed. Oxford University Press, Oxford.

    Google Scholar 

  19. 19.

    Heath, R. L., Packer, L. (1968) Photoperoxidation in isolated chloroplast. I. Kinetics and stochiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125, 189–198.

    CAS  Article  Google Scholar 

  20. 20.

    Janda, T., Szalai, G., Lesko, K., Yordanova, R., Apostol, S., Popova, L. P. (2007) Factors contributing to enhanced freezing tolerance in wheat during frost hardening in the light. Phytochem. 68, 1674–1682.

    CAS  Article  Google Scholar 

  21. 21.

    Jaspars, E. M. J. (1965) Pigmentation of tobacco crown-gall tissues cultured in vitro in dependence of the composition of the medium. Physiol. Plant. 18, 933–940.

    CAS  Article  Google Scholar 

  22. 22.

    Kadığu, A., Terzi, R. (2007) A dehydration avoidance mechanism: leaf rolling. The Bot. Rev. 73, 290–302.

    Article  Google Scholar 

  23. 23.

    Li, Q. M., Liu, B. B., Wu, Y., Zou, Z. R. (2008) Interactive effects of drought stresses and elevated CO2 concentration on photochemistry efficiency of cucumber seedlings. J. Intgr. Plant Biol. 50, 1–11.

    Article  Google Scholar 

  24. 24.

    Lichtenthaler, H. K., Rinderle, U. (1988) The role of chlorophyll fluorescence in detection of stress conditions in plants. Crit. Rev. Anal. Chem. 19, 529–585.

    Google Scholar 

  25. 25.

    Mittler, R., Zilinskas, B. A. (1994) Regulation of pea cytosolic ascorbate peroxidase and other antioxidant enzymes during the progression of drought and following recovery from drought. Plant J. 5, 397–405.

    CAS  Article  Google Scholar 

  26. 26.

    Nakano, Y., Asada, Y. (1987) Purification of ascorbate peroxidase from spinach chloroplasts: Its inactivation in ascorbat depleted medium and reactivation by monodehydroascorbate radical. Plant Cell Physiol. 28, 131–135.

    CAS  Google Scholar 

  27. 27.

    Nar, H., Sağlam, A., Terzi, R., Várkonyi, Z., Kadığlu, A. (2009) Leaf rolling and photosystem II efficiency in Ctenanthe setosa exposed to drought stress. Photosynthetika 47 (in press).

  28. 28.

    Niyogi, K. K. (1999) Photoprotection revisited: genetic and molecular approaches. Ann. Rev. Plant Phyiol. Plant Mol. Biol. 50, 333–359.

    CAS  Article  Google Scholar 

  29. 29.

    Oppenheimer, H. R. (1960) Plant Water Relationships in Arid and Semi-Arid Conditions, Unesco, UK, pp. 105–138.

    Google Scholar 

  30. 30.

    O’Toole, J. C., Moya, T. B. (1978) Genotypic variation in maintenance of leaf water potential in rice. Crop Sci. 18, 873–876.

    Article  Google Scholar 

  31. 31.

    Rensburg, L. V., Kruger, G. H. J. (1994) Evaluation of components of oxidative stress metabolism for use in selection of drought tolerant cultivars of Nicotiana tabacum L. J. Plant Physiol. 143, 730–737.

    Article  Google Scholar 

  32. 32.

    Sağlam, A., Kadıoğlu, A., Terzi, R., Saruhan, N. (2008) Physiological changes in them in post-stress emerging Ctenanthe setosa plants under drought conditions, Russ. J. Plant Physiol. 55, 48–53.

    Google Scholar 

  33. 33.

    Sairam, R. K., Shukla, D. S., Saxena, D. C. (1997/1998) Stress induced injury and antioxidant enzymes in relation to drought tolerance in wheat genotypes. Biol. Plantarum. 40, 357–364.

    CAS  Article  Google Scholar 

  34. 34.

    Sairam, R. K., Chandrasekhar, V., Srivastava, G. C. (2001) Comparison of hexaploid and tetraploid wheat cultivars in their responses to water stress. Biol. Plantarum. 44, 89–94.

    Article  Google Scholar 

  35. 35.

    Saruhan, N., Terzi, R., Sağlam, A., Nar, H., Kadıoğlu, A. (2010) Scavenging of reactive oxygen species in apoplastic and symplastic areas of rolled leaves in Ctenanthe setosa under drought stress. Acta Biol. Hung. 61 (in press).

  36. 36.

    Saruhan, N., Terzi, R., Sağlam, A., Kadıoğlu, A. (2009) The role of leaf rolling in regulation of ascorbate-glutathione cycle enzymes in apoplastic and symplastic areas of Ctenanthe setosa. Biol. Res. 42, 315–316.

    CAS  Article  Google Scholar 

  37. 37.

    Sharma, P., Dubey, S. (2005) Drought induces oxidative stres and enhances the activities of antioxidant enzymes in growing rice seedlings. Plant Growth Regul. 46, 209–221.

    CAS  Article  Google Scholar 

  38. 38.

    Terzi, R., Kadıoğlu, A. (2006) Drought stress tolerance and the antioxidant enzyme system in Ctenanthe setosa. Acta Biol. Cracov. Bot. 48, 89–96.

    Google Scholar 

  39. 39.

    Türkan, İ., Bor, M., Özdemir, F., Koca, H. (2005) Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Sci. 168, 223–231.

    Google Scholar 

  40. 40.

    van Kooten, O., Snel, J. F. H. (1990) The use of chlorophyll fluorescence nomenclature in plant stress physiology, Photosynth. Res. 25, 147–150.

    Article  Google Scholar 

  41. 41.

    Zhang, Y. J., Zhao, C. J., Liu, L. Y., Wang, J. H., Wang, R. C. (2005) Chlorophyll fluorescence detected passively by difference reflectance spectra of wheat (Triticum aestivum L.) leaf. J. Intgr. Plant Biol. 47, 1228–1235.

    CAS  Article  Google Scholar 

  42. 42.

    Zlatev, Z. S., Lidon, F. C., Ramalho, J. C., Yordanov, I. T. (2006). Comparison of resistance to drought of three bean cultivars. Biol. Plantarum. 50, 389–394.

    CAS  Article  Google Scholar 

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Affiliations

  1. Department of Biology, Faculty of Arts and Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey

    Rabiye Terzi, A. Sağam, Hatice Nar & A. Kadioğlu

  2. Department of Biology, Faculty of Arts and Sciences, Rize University, 53100, Rize, Turkey

    Neslihan Saruhan

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  1. Rabiye Terzi
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  2. Neslihan Saruhan
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  3. A. Sağam
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  4. Hatice Nar
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  5. A. Kadioğlu
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Correspondence to Neslihan Saruhan.

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Terzi, R., Saruhan, N., Sağam, A. et al. Photosystem II Functionality and Antioxidant System Changes During Leaf Rolling in Post-Stress Emerging Ctenanthe setosa Exposed to Drought. BIOLOGIA FUTURA 60, 417–431 (2009). https://doi.org/10.1556/ABiol.60.2009.4.8

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Keywords

  • Antioxidant enzymes
  • Ctenanthe setosa
  • chlorophyll fluorescence
  • leaf rolling
  • post-stress emerging plant