Advertisement

Acta Biologica Hungarica

, Volume 60, Issue 3, pp 301–307 | Cite as

Somatic Embryogenesis in Apomict Boechera holboellii

  • K. M. TaskinEmail author
  • K. Turgut
  • R. J. Scott
Article

Abstract

In this study we establish an efficient method for the regeneration for Boechera holboellii via somatic embryogenesis. Immature cotyledons from siliques of 4–6 month-old plants were cultured on MS medium supplemented with plant growth regulators (BA — 6-benzylaminopurine; NAA — α-naphthaleneacetic acid; TDZ — 1-phelyl-3-(1,2,3-thiadiazol-5-yl) urea: 2,4 D-2,4-dichlorophenoxy-acetic acid). A high frequency of embryogenic callus was produced after two weeks in culture. The somatic embryos were obtained with a frequency of 10% of explants on MS medium supplemented with 1.34 μM NAA + 8.87 μM BA and 2.68 μM NAA + 17.74 μM BA within 3 weeks in culture. The alternative regime of MS medium supplemented with 1.34 μM NAA + 4.44 μM BA produced somatic embryos at a frequency of 38%.

Keywords

Boechera Brassicaceae apomixis somatic embryogenesis tissue culture 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Notes

Acknowledgements

The authors thank The Scientific and Technical Research Council of Turkey (TUBITAK), Akdeniz University (Antalya-TURKEY), and The British Council for the financial support.

References

  1. 1.
    Bicknell, R. A., Koltunow, A. M. (2004) Understanding apomixis: Recent advances and remaining conundrums. Plant Cell. 16, S228–S245.CrossRefGoogle Scholar
  2. 2.
    Böcher, T. (1951) Cytological and embryological studies in the amphi-apomictic Arabis holboellii complex. Biologiske Skrifter. VI, 1–58.Google Scholar
  3. 3.
    Carman, J. G. (1997) Asynchronous expression of duplicate genes in angiosperms may cause apomixis, bispory, tetraspory, and polyembryony. Biol. J. Linnean Soc. 61, 51–94.CrossRefGoogle Scholar
  4. 4.
    Clough, S. J., Bent, A. F. (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 16, 735–743.CrossRefGoogle Scholar
  5. 5.
    Compton, M. E., Gray, D. J. (1993) Somatic embryogenesis and plant-regeneration from immature cotyledons of watermelon. Plant Cell Rep. 12, 61–65.CrossRefGoogle Scholar
  6. 6.
    Gaj, M. D. (2001) Direct somatic embryogenesis as a rapid and efficient system for in vitro regeneration of Arabidopsis thaliana. Plant Cell Tiss. Org. 64, 39–46.CrossRefGoogle Scholar
  7. 7.
    Koltunow, A. M. (1993) Apomixis–embryo sacs and embryos formed without meiosis or fertilization in ovules. Plant Cell 5, 1425–1437.CrossRefGoogle Scholar
  8. 8.
    Koltunow, A. M., Bicknell, R. A., Chaudhury, A. M. (1995) Apomixis–molecular strategies for the generation of genetically identical seeds without fertilization. Plant Physiol. 108, 1345–1352.CrossRefGoogle Scholar
  9. 9.
    Koltunow, A. M., Grossniklaus U. (2003) Apomixis: A developmental perspective. Annu. Rev. Plant Biol. 54, 547–574.CrossRefGoogle Scholar
  10. 10.
    Luo, Y. K., Koop, H. U. (1997) Somatic embryogenesis in cultured immature zygotic embryos and leaf protoplasts of Arabidopsis thaliana ecotypes. Planta 202, 387–396.CrossRefGoogle Scholar
  11. 11.
    Mohan, M. L., Krishnamurthy, K. V. (2002) Somatic embryogenesis and plant regeneration in pigeonpea. Biol. Plantarum 45, 19–25.CrossRefGoogle Scholar
  12. 12.
    Murashige, T., Skoog, A. (1962) A revised medium for rapid growth and bioassays with tabacco tissues culture. Plant Physiol. 15, 473–497.CrossRefGoogle Scholar
  13. 13.
    Naumova, T. N., Van der Laak, J., Osadtchiy, J., Matzk, F., Kravtchenko, A., Bergervoet, J., Ramulu, K. S., Boutilier, K. (2001) Reproductive development in apomictic populations of Arabis holboellii (Brassicaceae). Sexual Plant Reprod. 14, 195–200.CrossRefGoogle Scholar
  14. 14.
    Ozcan, S. (2002) Effect of different genotypes and auxins on efficient somatic embryogenesis from immature zygotic embryo explants of maize. Biotechnol. Biotech. Eq. 16, 51–57.Google Scholar
  15. 15.
    Ozias-Akins, P. (2006) Apomixis: Developmental characteristics and genetics. Critical Rev. Plant Sci. 25, 199–214.CrossRefGoogle Scholar
  16. 16.
    Roy, B. A., Rieseberg, L. H. (1989) Evidence for Apomixis in Arabis. J. Hered. 80, 506–508.Google Scholar
  17. 17.
    Schranz, M. E., Dobes, C., Koch, M. A., Mitchell-Olds, T. (2005) Sexual reproduction, hybridization, apomixis, and polyploidization in the genus Boechera (Brassicaceae). Am. J. Bot. 92, 1797–1810.CrossRefGoogle Scholar
  18. 18.
    Schranz, M. E., Kantama, L., de Jong, H., Mitchell-Olds, T. (2006) Asexual reproduction in a close relative of Arabidopsis: a genetic investigation of apomixis in Boechera (Brassicaceae). New Phytologist 171, 425–438.CrossRefGoogle Scholar
  19. 19.
    Spielman, M., Vinkenoog, R., Scott, R. J. (2003) Genetic mechanisms of apomixis. Phil. Trans. Royal Soc. Lond. B 358, 1095–1103.CrossRefGoogle Scholar
  20. 20.
    Taskin, K. M. et al. (2003) Agrobacterium-mediated transformation of Arabis gunnisoniana. Plant Cell Tiss. Org. 72, 173–179.CrossRefGoogle Scholar
  21. 21.
    Taskin, K. M., Turgut, K., Scott, R. J. (2004) Apomictic development in Arabis gunnisoniana. Israel J. Plant Sci. 52, 155–160.CrossRefGoogle Scholar
  22. 22.
    Turgut, K., Barghchi, M., Scott, J. R. (1998) Efficient shoot regeneration and somatic embryogenesis from immature cotyledons of Brassica napus L. Plant Breeding 117, 503–504.CrossRefGoogle Scholar
  23. 23.
    Vikrant, Rashid, A. (2001) Comparative study of somatic embryogenesis from immature and mature embryos and organogenesis from leaf-base of Triticale. Plant Cell Tiss. Org. 64, 33–38.CrossRefGoogle Scholar
  24. 24.
    Wu, Y., Haberland, G., Zhou, C., Koop, H. U. (1992) Somatic embryogenesis, formation of morphogenetic callus and normal development in zygotic embryos of Arabidopsis thaliana in vitro. Protoplasma 169, 89–96.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2009

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Department of BiologyCanakkale Onsekiz Mart UniversityCanakkaleTurkey
  2. 2.Department of Field CropsAkdeniz UniversityAntalyaTurkey
  3. 3.Department of Biology & BiochemistryUniversity of BathAvonEngland

Personalised recommendations