Somatic Embryogenesis in Apomict Boechera holboellii
In this study we establish an efficient method for the regeneration for Boechera holboellii via somatic embryogenesis. Immature cotyledons from siliques of 4–6 month-old plants were cultured on MS medium supplemented with plant growth regulators (BA — 6-benzylaminopurine; NAA — α-naphthaleneacetic acid; TDZ — 1-phelyl-3-(1,2,3-thiadiazol-5-yl) urea: 2,4 D-2,4-dichlorophenoxy-acetic acid). A high frequency of embryogenic callus was produced after two weeks in culture. The somatic embryos were obtained with a frequency of 10% of explants on MS medium supplemented with 1.34 μM NAA + 8.87 μM BA and 2.68 μM NAA + 17.74 μM BA within 3 weeks in culture. The alternative regime of MS medium supplemented with 1.34 μM NAA + 4.44 μM BA produced somatic embryos at a frequency of 38%.
KeywordsBoechera Brassicaceae apomixis somatic embryogenesis tissue culture
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The authors thank The Scientific and Technical Research Council of Turkey (TUBITAK), Akdeniz University (Antalya-TURKEY), and The British Council for the financial support.
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