Acta Biologica Hungarica

, Volume 60, Issue 3, pp 301–307 | Cite as

Somatic Embryogenesis in Apomict Boechera holboellii

  • K. M. TaskinEmail author
  • K. Turgut
  • R. J. Scott


In this study we establish an efficient method for the regeneration for Boechera holboellii via somatic embryogenesis. Immature cotyledons from siliques of 4–6 month-old plants were cultured on MS medium supplemented with plant growth regulators (BA — 6-benzylaminopurine; NAA — α-naphthaleneacetic acid; TDZ — 1-phelyl-3-(1,2,3-thiadiazol-5-yl) urea: 2,4 D-2,4-dichlorophenoxy-acetic acid). A high frequency of embryogenic callus was produced after two weeks in culture. The somatic embryos were obtained with a frequency of 10% of explants on MS medium supplemented with 1.34 μM NAA + 8.87 μM BA and 2.68 μM NAA + 17.74 μM BA within 3 weeks in culture. The alternative regime of MS medium supplemented with 1.34 μM NAA + 4.44 μM BA produced somatic embryos at a frequency of 38%.


Boechera Brassicaceae apomixis somatic embryogenesis tissue culture 


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The authors thank The Scientific and Technical Research Council of Turkey (TUBITAK), Akdeniz University (Antalya-TURKEY), and The British Council for the financial support.


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© Akadémiai Kiadó, Budapest 2009

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Department of BiologyCanakkale Onsekiz Mart UniversityCanakkaleTurkey
  2. 2.Department of Field CropsAkdeniz UniversityAntalyaTurkey
  3. 3.Department of Biology & BiochemistryUniversity of BathAvonEngland

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