Acta Biologica Hungarica

, Volume 60, Issue 2, pp 167–175 | Cite as

Effect of N-Metylcarbamate Pesticide Bendiocarb on Cattle Lymphocytes After in vitro Exposure

  • Beáta Holečková
  • Katarína ŠivikováEmail author
  • J. Dianovský


Bendiocarb is a carbamate broad-spectrum insecticide used to control disease vectors such as mosquitoes and flies, as well as household and agricultural pests. Nowadays, only few papers reporting cytogenetic or possible genotoxic effect of this insecticide on mammalian cells are available. In the present study 24-hour exposure to bendiocarbamate at concentrations ranging from 20 to 160 µg/ml was used for investigation of unstable chromosomal aberrations (CA), sister chromatid exchanges (SCE) and stable chromosomal aberration induction in cultured bovine peripheral lymphocytes. The slight but no significant increase of chromatide breaks frequency was observed after the exposure of lymphocytes to 80 µg/ml of bendiocarb. At the highest concentration added to the cell cultures (160 µg/ml) mitotic index decrease was shown in both donors (p < 0.05; p < 0.01). Both statistically significant elevation of SCEs (p < 0.05) and a reduction of proliferative indices (PI) (p < 0.01) were shown at a dose of 80 µg/ml. By means of two fluorescent-labelled whole chromosome-painting probes, stable aberrations such as bovine chromosome 1 and 5 translocation as well as numerical aberrations (polyploidies, heteroploidies) were visualised under fluorescent microscope in some examined metaphases.


Carbamate pesticides bovine lymphocytes chromosomal aberrations sister chromatide exchanges fluorescence in situ hybridisation 


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  1. 1.
    Albertini, R. J. (1998) The use and interpretation of biomarkers of environmental genotoxicity in humans. Biotherap. 11, 155–167.CrossRefGoogle Scholar
  2. 2.
    Ansari, M. A., Razdan, R. K. (2004) Impact of residual spraying of bendiocarb against the malaria vector Anopheles culicifacies in selected villages of the haziabad district, Uttarpradesh, India. J. Am. Mosq. Control Assoc. 20, 418–423.PubMedGoogle Scholar
  3. 3.
    Bueno, A. M. S., Pereira, C. A. B., Rabello-Gay, M. N. (2000) Environmental genotoxicity evaluation using cytogenetic end points in wild rodents. Environ. Health Perspect. 108, 1165–1169.CrossRefGoogle Scholar
  4. 4.
    Bull, S., Fletcher, K., Boobis, A. R., Battershill, J. M. (2006) Evidence for genotoxicity of pesicides in pesticide applicators: a review. Mutagenesi. 21, 93–103.CrossRefGoogle Scholar
  5. 5.
    EPA (1999) R. E. D. Facts: Bendiocarb. Office of Prevention, Pesticides and Toxic Substances, Washington, D. C., USA.Google Scholar
  6. 6.
    Fellows, M. D., O’Donovan, M. R. (2007) Cytotoxicity in cultured mammalian cells is a function of the method used to estimate it. Mutagenesi. 22, 275–280.CrossRefGoogle Scholar
  7. 7.
    Ganem, N. J., Storchova, Z., Pellman, D. (2007) Tetraploidy, aneuploidy and cancer. Curr Opin. Genetics Dev. 17, 157–162.CrossRefGoogle Scholar
  8. 8.
    Giri, S., Giri, A., Sharma, G. D., Prasad, S. B. (2002) Mutagenic effect of carbosulfan, a carbamate pesticide. Mutat. Res. 519, 75–82.CrossRefGoogle Scholar
  9. 9.
    Jaga, K., Dharmani, C. (2005) The epidemiology of pesticide exposure and cancer: a review. Rev. Environ. Health. 20, 15–38.CrossRefGoogle Scholar
  10. 10.
    Klotz, D. M., Arnold, S. F., McLachlan, J. A. (1997) Inhibition of 17 beta-estradiol and progesterone activity in human breast and endometrial cancer cells by carbamate insecticides. Life Sci. 60, 1467–1475.CrossRefGoogle Scholar
  11. 11.
    Lamberti, L., Ponzetto, B. P., Ardito, G. (1983) Cell kinetics and sister chromatid exchange frequency in human lymphocytes. Mutat. Res. 319, 193–199.CrossRefGoogle Scholar
  12. 12.
    Linn Ch. M., Wei, L. Y., Wang, T. Ch. (2007) The delayed genotoxic effect of N-nitroso N-propox-ur insecticide in mammalian cells. Food Chem. Toxicol. 45, 928–934.CrossRefGoogle Scholar
  13. 13.
    Maharaj, R., Casimiro, S., Mthembu, S. D., Sharp, B. I. (2004) The residual life of bendiocarb: A field-based evaluation from Mozambique. J. Med. Entomol. 41, 130–132.CrossRefGoogle Scholar
  14. 14.
    Matambo, T. S., Abdalla, H., Brooke, B. D., Koekemoer, L. L., Mnzava, A., Hunt, R. H., Coetzee, M. (2007) Insecticide resistance in the malarial mosquito Anopheles arabiensis and association with krd mutation. Med. Vet. Entomol. 21, 97–102.CrossRefGoogle Scholar
  15. 15.
    Natarajan, A. T. (2002) Chromosome aberrations: past, present and future. Mutat. Res. 504, 3–16.CrossRefGoogle Scholar
  16. 16.
    Pérez-Reuiz, T., Marzínez-Lozano, C, García, M. D. (2007) Determination of N-methylcarbamate pesticides in environmental samples by an automated solid-phase extraction and liquid chromatographic method based on post-column photolysis and chemiluminescence detection. J. Chromatogr. A. 1164, 174–180.CrossRefGoogle Scholar
  17. 17.
    Perry, P., Wolf, S. (1974) New Giemsa methods for differential staining of sister chromatids. Natur. 251, 156–158.CrossRefGoogle Scholar
  18. 18.
    Piešová, E., Valočíková, I. (2005) The activity of bendiocarbamate in the rabbit bone marrow micronucleus assay. Folia Vet. 49, 24–26.Google Scholar
  19. 19.
    Sánchez-Barragán, I., Karim, K., Costa-Fernández, 1 M, Piletsky, S. A., Sanz-Medel, A. (2007) A molecularly imprinted polymer for carbaryl determination in water. Sensors and Actuators B123, 798–804.CrossRefGoogle Scholar
  20. 20.
    Smulders, Ch. J. G. M., Bueters, T. J. H., Van Kleef, R. G. D. M., Vrjverberg, H. P. M. (2003) Selective effects of carbamate pesticides on rat neuronal nicotinic acetylcholine receptors and rat brain acetylcholine esterase. Toxicol. Appl. Pharm. 193, 139–146.CrossRefGoogle Scholar
  21. 21.
    Tucker, J. D., Morgan, W. F., Awa, A. A., Bauchinger, M., Blakey, D., Cornforth, M. N., Littlefield, L. G., Natarajan, A. T., Shasserre, C. (1995) A proposed system for scoring structural aberrations detected by chromosome painting. Cytogenet. Cell Genet. 68, 211–221.CrossRefGoogle Scholar
  22. 22.
    Vasudev, V., Krishnamurthy, N. B. (1994) In vivo cytogenetic analysis of the carbamate pesticides Dithane M-45 and Baygon in mice. Mutat. Res. 323, 133–135.CrossRefGoogle Scholar
  23. 23.
    Wang, T. C., Chiou, C. M., Chang, T. L. (1998) Genetic toxicity of N-methylcarbamate insecticides and their N-nitroso derivatives. Mutagenesi. 13, 405–408.CrossRefGoogle Scholar
  24. 24.
    Zhakhovghirov, Sh. M., Abdullaev, I. T., Ponomaev, I. M., Muminov, M. S. (2004) Monitoring of the insecticidal resistance of main malaria vectors in Uzbekistan. Med. Parazitol. (Mosk. 1, 29–33.Google Scholar

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© Akadémiai Kiadó, Budapest 2009

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Authors and Affiliations

  • Beáta Holečková
    • 1
  • Katarína Šiviková
    • 1
    Email author
  • J. Dianovský
    • 1
  1. 1.Department of Biology and GeneticsUniversity of Veterinary MedicineKošiceSlovak Republic

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