A Novel Poly(3-Hydroxybutyrate)-Degrading Streptoverticillium Kashmirense AF1 Isolated from Soil and Purification of Phb-Depolymerase

Abstract

A new bacterial strain, capable of degrading poly(3-hydroxybutyrate) (PHB) was isolated from soil. This organism, identified as Streptoverticillium kashmirense AF1, secreted PHB depolymerases both on solid as well as in liquid mineral salt medium containing poly(3-hydroxybutyrate) as sole carbon source. The optimum production of PHB depolymerase was observed at pH 8 and 7, at 45 °C, 1% substrate concentration and in the presence of lactose as an additional carbon source. The extracellular PHB depolymerase was purified by gel permeation chromatography using Sephadex G-75. The Streptoverticillium kashmirense AF1 produced two types of PHB depolymerases having molecular weights of about 37 and 45 kDa as determined by SDS-PAGE. The difference in dry cell mass and amount of CO2 evolved in the test and control calculated gravimetrically through Sturm test indicated the degradative capabilities of Streptoverticillium kashmirense AF1.

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Correspondence to A. A. Shah.

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Shah, A.A., Hasan, F., Hameed, A. et al. A Novel Poly(3-Hydroxybutyrate)-Degrading Streptoverticillium Kashmirense AF1 Isolated from Soil and Purification of Phb-Depolymerase. BIOLOGIA FUTURA 59, 489–499 (2008). https://doi.org/10.1556/ABiol.59.2008.4.9

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Keywords

  • Biodegradation
  • poly(3-hydroxybutyrate)
  • poly(3-hydroxybutyrate) depolymerase
  • Streptoverticillium kashmirense AF1
  • purification