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Acta Biologica Hungarica

, Volume 59, Issue 4, pp 489–499 | Cite as

A Novel Poly(3-Hydroxybutyrate)-Degrading Streptoverticillium Kashmirense AF1 Isolated from Soil and Purification of Phb-Depolymerase

  • A. A. ShahEmail author
  • Fariha Hasan
  • A. Hameed
  • Safia Ahmed
Article

Abstract

A new bacterial strain, capable of degrading poly(3-hydroxybutyrate) (PHB) was isolated from soil. This organism, identified as Streptoverticillium kashmirense AF1, secreted PHB depolymerases both on solid as well as in liquid mineral salt medium containing poly(3-hydroxybutyrate) as sole carbon source. The optimum production of PHB depolymerase was observed at pH 8 and 7, at 45 °C, 1% substrate concentration and in the presence of lactose as an additional carbon source. The extracellular PHB depolymerase was purified by gel permeation chromatography using Sephadex G-75. The Streptoverticillium kashmirense AF1 produced two types of PHB depolymerases having molecular weights of about 37 and 45 kDa as determined by SDS-PAGE. The difference in dry cell mass and amount of CO2 evolved in the test and control calculated gravimetrically through Sturm test indicated the degradative capabilities of Streptoverticillium kashmirense AF1.

Keywords

Biodegradation poly(3-hydroxybutyrate) poly(3-hydroxybutyrate) depolymerase Streptoverticillium kashmirense AF1 purification 

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© Akadémiai Kiadó, Budapest 2008

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • A. A. Shah
    • 1
    Email author
  • Fariha Hasan
    • 1
  • A. Hameed
    • 1
  • Safia Ahmed
    • 1
  1. 1.Department of MicrobiologyQuaid-i-Azam UniversityIslamabadPakistan

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