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Acta Biologica Hungarica

, Volume 59, Issue 4, pp 453–464 | Cite as

Effects of Hyperbaric Oxygenation on Cultured Barley Embryos

  • E. AricanEmail author
  • N. Gozukirmizi
Open Access
Article

Abstract

Changes in relative water content (RWC), lipid peroxidation, proline and antioxidant system in relation to the tolerance to oxidative stress enzymes mediated high pressure were investigated in Hordeum vul-gare L. cv. Tokak. For this purpose, mature embryos cultured on MS media were treated in a hyperbaric oxygenation chamber (approx. 59.06 feets, 2 kp/cm2) with pure oxygen for 60 minutes/day for a growth period of ten days in a plant growth chamber. Constitutive activities of SOD, APOX, GR and POX were higher in hyperbaric oxygenated (HBO) explants, being 96.07%, 28.57%, 77.77% and 54.14% for the 5th days; 95.78%, 40%, 37.5%, and 94.98% for the 10th days of culture, respectively, than in the control plants. Increase in SOD activity was also shown on polyacrilamide gel electrophoresis on the 10th day of application. Proline accumulation was increased in HBO-treated explants both on the 5th days (85.71%) and 10th days (37.14%) of treatment. MDA content was found to be higher in HBO treated explants both on the 5th (53.84%) and 10th (59.83%) days of culture.

Keywords

Antioxidant enzymes lipid peroxidation Hordeum vulgarehyperbaric oxygenation oxidative stress relative water content 

Abbreviations

APOX

ascorbate peroxidase

CAT

catalase

GR

glutathione reductase

HBO

hyperbaric oxygenation

MDA

malondialdehyde

MS

Murashige and Skoog medium

POX

peroxidase

RWC

relative water content

SOD

superoxide dismutase

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© Akadémiai Kiadó, Budapest 2008

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Department of Molecular Biology and Genetics, Faculty of ScienceIstanbul UniversityVezneciler-IstanbulTurkey

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