Acta Biologica Hungarica

, Volume 59, Issue 4, pp 453–464 | Cite as

Effects of Hyperbaric Oxygenation on Cultured Barley Embryos

  • E. AricanEmail author
  • N. Gozukirmizi
Open Access


Changes in relative water content (RWC), lipid peroxidation, proline and antioxidant system in relation to the tolerance to oxidative stress enzymes mediated high pressure were investigated in Hordeum vul-gare L. cv. Tokak. For this purpose, mature embryos cultured on MS media were treated in a hyperbaric oxygenation chamber (approx. 59.06 feets, 2 kp/cm2) with pure oxygen for 60 minutes/day for a growth period of ten days in a plant growth chamber. Constitutive activities of SOD, APOX, GR and POX were higher in hyperbaric oxygenated (HBO) explants, being 96.07%, 28.57%, 77.77% and 54.14% for the 5th days; 95.78%, 40%, 37.5%, and 94.98% for the 10th days of culture, respectively, than in the control plants. Increase in SOD activity was also shown on polyacrilamide gel electrophoresis on the 10th day of application. Proline accumulation was increased in HBO-treated explants both on the 5th days (85.71%) and 10th days (37.14%) of treatment. MDA content was found to be higher in HBO treated explants both on the 5th (53.84%) and 10th (59.83%) days of culture.


Antioxidant enzymes lipid peroxidation Hordeum vulgarehyperbaric oxygenation oxidative stress relative water content 



ascorbate peroxidase




glutathione reductase


hyperbaric oxygenation




Murashige and Skoog medium




relative water content


superoxide dismutase


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© Akadémiai Kiadó, Budapest 2008

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Authors and Affiliations

  1. 1.Department of Molecular Biology and Genetics, Faculty of ScienceIstanbul UniversityVezneciler-IstanbulTurkey

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