Zinc Content in the Diet Affects the Activity of Cu/ZnSOD, Lipid Peroxidation and Lipid Profile of Spontaneously Hypertensive Rats
Abstract
The present study focused on the effect of Zn containing diets on the activity of superoxide dismutase (Cu/ZnSOD), systolic blood pressure (SBP), lipid peroxides (ROOH) and lipids (LDL, HDL, triglycerides and cholesterol) in male spontaneously hypertensive rats (SHR). Three experimental groups of animals were studied: a control (G1-40 mg), and two with zinc-supplemented diets (G2-100 and G3-160 mg Zn/kg lab chow). The diets were introduced at the beginning of the development of hypertension (2 months after birth) and the animals were fed for 8 weeks. The activity of CuZnSOD in erythrocytes was determined by spectrophotometry with the use of RANSOD kit (RANDOX Laboratories Ltd., UK). Atomic-absorption spectrometry was used to determine Zn and Cu concentrations in the rat’s sera. A significantly increased Cu/ZnSOD activity was found in G3 compared with rats fed with control diet G. (p = 0.020). SBP was significantly decreased in G3 in relation to G. (p = 0.0048). The lipid hydroperoxide concentration was significantly decreased in G3 compared with G. (p = 0.016) and G. (p = 0.005). Zinc supplement affected lipids profile by decreasing LDL and increasing HDL. The present data suggest that Zn concentration in the diet plays an important role in the regulation of SBP and can be a critical nutrient for maintenance of anti-oxidative events in SHR.
Keywords
Zinc diet SHR Cu/ZnSOD lipid hydroperoxides systolic blood pressurePreview
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References
- 1.Apostolova, M., Nachev, C., Koleva, M., Bontchev, P. R., Kehaiov, I. (1998) New competitive enzyme-linked immunosorbent assay for determination of metallothionein in tissue and sera. Talanta 46, 325–333.CrossRefGoogle Scholar
- 2.Black, M. R., Medeiros, D. M., Brunett, E., Welke, R. (1988) Zinc supplements and serum lipids in young adult white males. Am. J. Clin. Nutr. 47, 970–975.CrossRefGoogle Scholar
- 3.Bray, T. M., Bettger, W. J. (1990) The physiological role of zinc as an antioxidant. Free Radic. Biol. Med. 8, 281–291.CrossRefGoogle Scholar
- 4.Cabell, K. S., Ma, L., Johnson, P. (1997) Effects of antihypertensive drugs on rat tissue antioxidant enzyme activities and lipid peroxidation levels. Biochem. Pharmacol. 54, 133–141.CrossRefGoogle Scholar
- 5.Cebeci, S. A., Kocuturk, P. A., Kavas, G. O. (2002) Hypertension: does impaired endothelium-depen-dent relaxation affect superoxide scavenging. Biol. Trace Elem. Res. 90, 239–249.CrossRefGoogle Scholar
- 6.Cousins, R. J. (1995) Absorption, transport and hepatic metabolism of copper and zinc: special reference to metalothionein and ceruloplasmin. Phys. Rev. 65, 238–309.Google Scholar
- 7.Cunnane, S. C. (1988) Role of zinc in lipid and fatty acid metabolism and in membranes. Prog. Food Nutr. Sci. 12, 151–188.PubMedGoogle Scholar
- 8.Di Silvestro, R. (2000) Zinc in relation to diabetes and oxidative disease. J. Nutr. 130, 1509–1511.CrossRefGoogle Scholar
- 9.Faure, P., Roussel, A. M., Richard, M. J., Foulon, T., Groslambert, P., Hadjian, A., Favier, A. (1991) Effect of an acute zinc depletion on rat lipoprotein distribution and peroxidation. Biol. Trace Elem. Res. 28, 135–146.CrossRefGoogle Scholar
- 10.Hennig, B., Toborec, M, McClain, J. C. (1999) Antioxidant-like properties of zinc in activated endothelial cells. J. Amer. Coll. Nutr. 18, 152–158.CrossRefGoogle Scholar
- 11.Khoja, S. M., Marzouki, Z. M., Ashry, K. M. et al. (2002) Effect of dietary zinc deficiency on rat lipid concentrations. Saudi Med. J. 23, 82–86.PubMedGoogle Scholar
- 12.Koo, S. I., Williams, D. A. (1981) Relationship between the nutritional status of zinc and cholesterol concentration of serum lipoproteins in adult male rats. Am. J. Clin. Nutr. 34, 2376–2381.CrossRefGoogle Scholar
- 13.McCord, J. M., Fridovich, J. (1969) Superoxide dismutase. An enzymatic function for Erythro-cuprein. J. Biol. Chem. 244, 6049–6055.PubMedGoogle Scholar
- 14.Mertens, A., Holvoet, P. (2001) Oxidized LDL and HDL: antagonists in atherothrombosis. FASEB J. 15, 2073–2084.CrossRefGoogle Scholar
- 15.Miller, A. F. (2004) Superoxide dismutases: active sites that save, but a protein that kills. Curr. Opin. Chem. Biol. 8, 162–168.CrossRefGoogle Scholar
- 16.Oteiza, P. L., Olin, K. L., Fraga, C. G., Keen, C. L. (1996) Oxidant defense systems in testes from zinc deficient rats. Proc. Soc. Exp. Biol. Med. 213, 85–91.CrossRefGoogle Scholar
- 17.Paul, C. F. (1991) Lipids, lipoproteins and apolipoproteins. In: Howanitz, S. H., Howanitz, P. S. (eds). Laboratory Medicine. Test Selection and Interpretation. New York, Edinburgh, London, Melburne, Tokyo.Google Scholar
- 18.Roughead, Z. K., Johnson, L. K., Hunt, J. R. (1999) Dietary copper primarily affects antioxidant capacity and dietary iron mainly affects iron status in a surface response study of female rats fed varying concentrations of iron, zinc and coppe. J. Nutr. 129, 1368–1376.CrossRefGoogle Scholar
- 19.Russo, C., Olivieri, O., Girelli, D., Faccini, G., Zenari, M. L., Lombardi, S., Corrocher, R. (1998) Anti-oxidant status of lipid peroxidation in patients with essential hypertension. J. Hypertens. 16, 1267–1271.CrossRefGoogle Scholar
- 20.Sakanashi, T. M., Keen, C. L., Hong, K. H. et al. (1993) Zinc deficiency associated alterations in the chemotactic and respiratory burst responses of rat peripheral blood neutrophils. FASEB J. 7, A723.CrossRefGoogle Scholar
- 21.Sato, M., Yanagisawa, H., Nojima, Y. et al. (2002) Zn deficiency aggravates hypertension in spontaneously hypertensive rats: Possible role of Cu/ZnSOD. Clin. Exp. Hypertens. 24, 355–370.CrossRefGoogle Scholar
- 22.Shaheen, A., Abd El-Fattah, A. (1995) Effect of dietary zinc on lipid peroxidation, glutathione, protein thiols levels and superoxide dismutase activity in rat tissues. Int. J. Biochem. Cell Biol. 27, 89–95.CrossRefGoogle Scholar
- 23.Yagi, K. (1982) Assay for serum and lipid peroxide level and its clinical significance. In: Yagi, K. (ed.). Lipid Peroxides in Biology and Medicine. Academic Press, Orlando, pp. 223–243.CrossRefGoogle Scholar
- 24.Yanagisawa, H., Moridaira, K., Wada, O. (2000) Zinc deficiency further increases the enhanced expression of endothelin-1 in glomeruli of the obstricted kidney. Kidney Int. 58, 575–586.CrossRefGoogle Scholar
- 25.Yelinova, V. I., Khramtsov, V. V., Markel, A. L. (1999) Manifestation of oxidative stress in the pathogenesis of arterial hypertension in ISIAH rats. Biochem. Biophys. Res. Commun. 263, 450–453.CrossRefGoogle Scholar
- 26.Yokoi, K., Alcock, N. W., Sandstead, H. H. (1994) Iron and zinc nutriture of premenopausal women: Associations of diet with serum ferritin and plasma zinc disappearance. J. Lab. Clin. Med. 124, 852–861.PubMedGoogle Scholar
- 27.Yousef, M. I., El-Hendy, H. A., El-Demerdash, F. M. et al. (2002) Dietary zinc deficiency induced-changes in the activity of enzymes and the levels of free radicals, lipids and protein electrophoretic behavior in growing rats. Toxicology 14, 223–234.CrossRefGoogle Scholar
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