The present study focused on the effect of Zn containing diets on the activity of superoxide dismutase (Cu/ZnSOD), systolic blood pressure (SBP), lipid peroxides (ROOH) and lipids (LDL, HDL, triglycerides and cholesterol) in male spontaneously hypertensive rats (SHR). Three experimental groups of animals were studied: a control (G1-40 mg), and two with zinc-supplemented diets (G2-100 and G3-160 mg Zn/kg lab chow). The diets were introduced at the beginning of the development of hypertension (2 months after birth) and the animals were fed for 8 weeks. The activity of CuZnSOD in erythrocytes was determined by spectrophotometry with the use of RANSOD kit (RANDOX Laboratories Ltd., UK). Atomic-absorption spectrometry was used to determine Zn and Cu concentrations in the rat’s sera. A significantly increased Cu/ZnSOD activity was found in G3 compared with rats fed with control diet G. (p = 0.020). SBP was significantly decreased in G3 in relation to G. (p = 0.0048). The lipid hydroperoxide concentration was significantly decreased in G3 compared with G. (p = 0.016) and G. (p = 0.005). Zinc supplement affected lipids profile by decreasing LDL and increasing HDL. The present data suggest that Zn concentration in the diet plays an important role in the regulation of SBP and can be a critical nutrient for maintenance of anti-oxidative events in SHR.
Apostolova, M., Nachev, C., Koleva, M., Bontchev, P. R., Kehaiov, I. (1998) New competitive enzyme-linked immunosorbent assay for determination of metallothionein in tissue and sera. Talanta 46, 325–333.
Black, M. R., Medeiros, D. M., Brunett, E., Welke, R. (1988) Zinc supplements and serum lipids in young adult white males. Am. J. Clin. Nutr. 47, 970–975.
Bray, T. M., Bettger, W. J. (1990) The physiological role of zinc as an antioxidant. Free Radic. Biol. Med. 8, 281–291.
Cabell, K. S., Ma, L., Johnson, P. (1997) Effects of antihypertensive drugs on rat tissue antioxidant enzyme activities and lipid peroxidation levels. Biochem. Pharmacol. 54, 133–141.
Cebeci, S. A., Kocuturk, P. A., Kavas, G. O. (2002) Hypertension: does impaired endothelium-depen-dent relaxation affect superoxide scavenging. Biol. Trace Elem. Res. 90, 239–249.
Cousins, R. J. (1995) Absorption, transport and hepatic metabolism of copper and zinc: special reference to metalothionein and ceruloplasmin. Phys. Rev. 65, 238–309.
Cunnane, S. C. (1988) Role of zinc in lipid and fatty acid metabolism and in membranes. Prog. Food Nutr. Sci. 12, 151–188.
Di Silvestro, R. (2000) Zinc in relation to diabetes and oxidative disease. J. Nutr. 130, 1509–1511.
Faure, P., Roussel, A. M., Richard, M. J., Foulon, T., Groslambert, P., Hadjian, A., Favier, A. (1991) Effect of an acute zinc depletion on rat lipoprotein distribution and peroxidation. Biol. Trace Elem. Res. 28, 135–146.
Hennig, B., Toborec, M, McClain, J. C. (1999) Antioxidant-like properties of zinc in activated endothelial cells. J. Amer. Coll. Nutr. 18, 152–158.
Khoja, S. M., Marzouki, Z. M., Ashry, K. M. et al. (2002) Effect of dietary zinc deficiency on rat lipid concentrations. Saudi Med. J. 23, 82–86.
Koo, S. I., Williams, D. A. (1981) Relationship between the nutritional status of zinc and cholesterol concentration of serum lipoproteins in adult male rats. Am. J. Clin. Nutr. 34, 2376–2381.
McCord, J. M., Fridovich, J. (1969) Superoxide dismutase. An enzymatic function for Erythro-cuprein. J. Biol. Chem. 244, 6049–6055.
Mertens, A., Holvoet, P. (2001) Oxidized LDL and HDL: antagonists in atherothrombosis. FASEB J. 15, 2073–2084.
Miller, A. F. (2004) Superoxide dismutases: active sites that save, but a protein that kills. Curr. Opin. Chem. Biol. 8, 162–168.
Oteiza, P. L., Olin, K. L., Fraga, C. G., Keen, C. L. (1996) Oxidant defense systems in testes from zinc deficient rats. Proc. Soc. Exp. Biol. Med. 213, 85–91.
Paul, C. F. (1991) Lipids, lipoproteins and apolipoproteins. In: Howanitz, S. H., Howanitz, P. S. (eds). Laboratory Medicine. Test Selection and Interpretation. New York, Edinburgh, London, Melburne, Tokyo.
Roughead, Z. K., Johnson, L. K., Hunt, J. R. (1999) Dietary copper primarily affects antioxidant capacity and dietary iron mainly affects iron status in a surface response study of female rats fed varying concentrations of iron, zinc and coppe. J. Nutr. 129, 1368–1376.
Russo, C., Olivieri, O., Girelli, D., Faccini, G., Zenari, M. L., Lombardi, S., Corrocher, R. (1998) Anti-oxidant status of lipid peroxidation in patients with essential hypertension. J. Hypertens. 16, 1267–1271.
Sakanashi, T. M., Keen, C. L., Hong, K. H. et al. (1993) Zinc deficiency associated alterations in the chemotactic and respiratory burst responses of rat peripheral blood neutrophils. FASEB J. 7, A723.
Sato, M., Yanagisawa, H., Nojima, Y. et al. (2002) Zn deficiency aggravates hypertension in spontaneously hypertensive rats: Possible role of Cu/ZnSOD. Clin. Exp. Hypertens. 24, 355–370.
Shaheen, A., Abd El-Fattah, A. (1995) Effect of dietary zinc on lipid peroxidation, glutathione, protein thiols levels and superoxide dismutase activity in rat tissues. Int. J. Biochem. Cell Biol. 27, 89–95.
Yagi, K. (1982) Assay for serum and lipid peroxide level and its clinical significance. In: Yagi, K. (ed.). Lipid Peroxides in Biology and Medicine. Academic Press, Orlando, pp. 223–243.
Yanagisawa, H., Moridaira, K., Wada, O. (2000) Zinc deficiency further increases the enhanced expression of endothelin-1 in glomeruli of the obstricted kidney. Kidney Int. 58, 575–586.
Yelinova, V. I., Khramtsov, V. V., Markel, A. L. (1999) Manifestation of oxidative stress in the pathogenesis of arterial hypertension in ISIAH rats. Biochem. Biophys. Res. Commun. 263, 450–453.
Yokoi, K., Alcock, N. W., Sandstead, H. H. (1994) Iron and zinc nutriture of premenopausal women: Associations of diet with serum ferritin and plasma zinc disappearance. J. Lab. Clin. Med. 124, 852–861.
Yousef, M. I., El-Hendy, H. A., El-Demerdash, F. M. et al. (2002) Dietary zinc deficiency induced-changes in the activity of enzymes and the levels of free radicals, lipids and protein electrophoretic behavior in growing rats. Toxicology 14, 223–234.
About this article
Cite this article
Dimitrova, A.A., Strashimirov, D., Betova, T. et al. Zinc Content in the Diet Affects the Activity of Cu/ZnSOD, Lipid Peroxidation and Lipid Profile of Spontaneously Hypertensive Rats. BIOLOGIA FUTURA 59, 305–314 (2008). https://doi.org/10.1556/ABiol.59.2008.3.4
- Zinc diet
- lipid hydroperoxides
- systolic blood pressure