Acta Biologica Hungarica

, Volume 59, Issue 3, pp 305–314 | Cite as

Zinc Content in the Diet Affects the Activity of Cu/ZnSOD, Lipid Peroxidation and Lipid Profile of Spontaneously Hypertensive Rats

  • Anelia A. DimitrovaEmail author
  • D. Strashimirov
  • Tatiana Betova
  • Adelaida Russeva
  • Margarita Alexandrova


The present study focused on the effect of Zn containing diets on the activity of superoxide dismutase (Cu/ZnSOD), systolic blood pressure (SBP), lipid peroxides (ROOH) and lipids (LDL, HDL, triglycerides and cholesterol) in male spontaneously hypertensive rats (SHR). Three experimental groups of animals were studied: a control (G1-40 mg), and two with zinc-supplemented diets (G2-100 and G3-160 mg Zn/kg lab chow). The diets were introduced at the beginning of the development of hypertension (2 months after birth) and the animals were fed for 8 weeks. The activity of CuZnSOD in erythrocytes was determined by spectrophotometry with the use of RANSOD kit (RANDOX Laboratories Ltd., UK). Atomic-absorption spectrometry was used to determine Zn and Cu concentrations in the rat’s sera. A significantly increased Cu/ZnSOD activity was found in G3 compared with rats fed with control diet G. (p = 0.020). SBP was significantly decreased in G3 in relation to G. (p = 0.0048). The lipid hydroperoxide concentration was significantly decreased in G3 compared with G. (p = 0.016) and G. (p = 0.005). Zinc supplement affected lipids profile by decreasing LDL and increasing HDL. The present data suggest that Zn concentration in the diet plays an important role in the regulation of SBP and can be a critical nutrient for maintenance of anti-oxidative events in SHR.


Zinc diet SHR Cu/ZnSOD lipid hydroperoxides systolic blood pressure 


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© Akadémiai Kiadó, Budapest 2008

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Anelia A. Dimitrova
    • 1
    Email author
  • D. Strashimirov
    • 1
  • Tatiana Betova
    • 2
  • Adelaida Russeva
    • 3
  • Margarita Alexandrova
    • 4
  1. 1.Department of PathophysiologyMedical UniversityPlevenBulgaria
  2. 2.Department of PathoanatomyMedical UniversityPlevenBulgaria
  3. 3.Department of Clinical LaboratoryMedical UniversityPlevenBulgaria
  4. 4.Department of BiophysicsMedical UniversityPlevenBulgaria

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