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Acta Biologica Hungarica

, Volume 59, Issue 2, pp 147–161 | Cite as

Postnatal Expression of Doublecortin (Dcx) in the Developing Cerebellar Cortex of Mouse

  • J. TakácsEmail author
  • Roberta Zaninetti
  • Julianna Víg
  • Cs. Vastagh
  • J. Hámori
Article

Abstract

We have investigated the expression of Doublecortin (Dcx) protein in the developing cerebellum of mouse from postnatal 2nd day to postnatal 22nd day and in young adults by immunohistochemistry. Strong expression of Dcx was present in the inner zone of the external granule cell layer, and remained strong while postmitotic granule cell precursors were present in this transitory layer. Descending granule cell precursors exhibited Dcx immunostaining not only while migrating but for a short time also after their settlement. Dcx-immunostained cells appeared in deep cerebellocortical territories and in the cerebellar white matter during the first postnatal week. These bipolar cells were arranged in the sagittal plane and built up transitory migratory streams during the second postnatal week and their number gradually decreased during the third postnatal week. Upward migration of bipolar cells was observed while leaving the migratory streams, penetrating the internal granule cell layer and the molecular layer. These cells were considered as precursors of late migrating molecular layer interneurons. However, a proportion of Dcx-immunostained cells underwent a bipolar-to-multipolar dendritic remodellation and — on the basis of strong morphological similarities — was taken for “multipotent progenitor cells”, described recently in the neocortex of adult rat.

Keywords

Cerebellum development neuronal migration remodeling 

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Notes

Acknowledgements

We are grateful to Dr. R. Maggi for critical reading of successive versions of the manuscript, and thank for providing Doublecortin (C-18) antibody. This work was supported by OTKA grant T 43478. The Hungarian Science and Technology Foundation together with the Italian Ministry of Foreign Affairs also supported this work in the frame of Hungarian—Italian Intergovernmental Scientific and Technology Cooperation, I-14/03.

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© Akadémiai Kiadó, Budapest 2008

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • J. Takács
    • 1
    Email author
  • Roberta Zaninetti
    • 2
  • Julianna Víg
    • 1
  • Cs. Vastagh
    • 3
  • J. Hámori
    • 1
  1. 1.Infobionic and Neurobiological Plasticity Research Group of the Hungarian Academy of SciencesPéter Pázmány Catholic University - Semmelweis UniversityBudapestHungary
  2. 2.Laboratory of Developmental Neuroendocrinology, Department of Endocrinology, Centre of Excellence on Neurodegenerative DiseasesUniversity of MilanoMilanoItaly
  3. 3.Institute of Experimental MedicineHungarian Academy of SciencesBudapestHungary

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