Advertisement

Acta Biologica Hungarica

, Volume 59, Issue 1, pp 93–102 | Cite as

Effects of Polyamines and Polyamine Biosynthetic Inhibitors on Mitotic Activity of Allium Cepa Root Tips

  • Meral Unal
  • Narcin Palavan-Unsal
  • M. A. TufekciEmail author
Open Access
Article

Abstract

The genotoxic and cytotoxic effects of exogenous polyamines (PAs), putrescine (Put), spermidine (Spd), spermine (Spm) and PA biosynthetic inhibitors, α-difluoromethylornithine (DFMO), cyclohexilamine (CHA), methylglioxal bis-(guanylhydrazone) (MGBG) were investigated in the root meristems of Allium cepa L. The reduction of mitotic index and the induction of chromosomal aberrations such as bridges, stickiness, c-mitotic anaphases, micronuclei, endoredupliction by PAs and PA biosynthetic inhibitors were observed and these were used as evidence of genotoxicity and cytotoxicity.

Keywords

Allium cepa chromosome aberration mitotic activity polyamine polyamine biosynthetic inhibitor 

References

  1. 1.
    Anehus S., Pohjanpelto P., Baldetorp B., Langstrom E., Heby O. (1984) Polyamine starvation prolongs the S and G2 phases of polyamine-dependent (arginase-deficient) CHO cells. Mol. Cell. Biol. 4, 915–922.CrossRefGoogle Scholar
  2. 2.
    Bharti R., Rajam M. V. (1995) Effect of polyamine biosynthesis inhibitor difluoromethyl-ornithine on growth, polyamine levels, chromosome behaviour and polygenic traits in wheat (Triticum aes-tivum L.). Ann. Botany. 76, 297–301.CrossRefGoogle Scholar
  3. 3.
    Bloomfield V. A. (2000) Static and dynamic light scattering from aggregating particles. Biopolymers 54, 168–172.CrossRefGoogle Scholar
  4. 4.
    Cebolla J. M., Vinardell E., Kiss B., Olah, E., Roudier A., Kondorosi E. (1999) The mitotic inhibitor ccs52 is required for endoreduplication and ploidy-depent cell enlargement in plants. The EMBO J. 18, 4476–4484.CrossRefGoogle Scholar
  5. 5.
    de Agazio M., Federico R., Angelini R., De Cesare F., Grego S. (1992) Spermidine pretreatment or root tip removal in maize seedlings: effect on K+ uptake and tissue modifications. J. Plant Physiol. 140, 741–746.CrossRefGoogle Scholar
  6. 6.
    de Agazio M., Grego S., Ciofi-Luzzatto A., Rea E., Zaccaria M. L., Federico R. (1995) Inhibition of maize primary root elongation by spermidine: effect on cell shape and mitotic index. J. Plant Growth Regul. 14, 85–89.CrossRefGoogle Scholar
  7. 7.
    Evans P. T., Malmberg R. L. (1989) Do polyamines have roles in plant development? Annu. Rev. Plant Physiol. Plant Mol. Biol. 40, 235–269.CrossRefGoogle Scholar
  8. 8.
    Feuerstein B. G., Pattabiraman N., Marton L. C. (1989) Molecular dynamics of spermine-DNA interactions: sequence specifity and DNA bending for a simple ligand. Nucleic Acids Res. 17, 6883–6892.CrossRefGoogle Scholar
  9. 9.
    Gatta L. I., Marchitelli, C., Federico R. (1992) Effect of polyamines and their oxidative products on maize and lentil root growth. Ann. Botany (Italy) 50, 43–48.Google Scholar
  10. 10.
    Goyns M. H. (1979) Polyamine content of non-aqueously isolated chromosome preparation. Exp. CellRes. 122, 377–380.CrossRefGoogle Scholar
  11. 11.
    Hougaard D. M. (1992) Polyamine cytochemistry: localization and possible functions of polyamines. Int. Rev. Cytol. 138, 51–88.CrossRefGoogle Scholar
  12. 12.
    Hougaard D. M., Blound L., Fujiwara K., Larsson L. I. (1987) Endogenous polyamines are intimately associated with highly condensed chromatin in-vivo. A fluorescence cytochemical and immunocytochemical study of spermine and spermidine during the cell cycle and in reactivated nuclei. EJCB 44, 151–155.Google Scholar
  13. 13.
    Kaur-Sawhney R., Flores H. E., Galston A. W. (1980) Polyamine-induced DNA synthesis and mitosis in oat leaf protoplast. Plant Physiol. 65, 368–371.CrossRefGoogle Scholar
  14. 14.
    Liu D., Jiang W., Gao X. (2003) Effects of cadmium on root growth, cell division and nucleoli in root tip cells of garlic. Biol. Plantarum. 47, 79–83.CrossRefGoogle Scholar
  15. 15.
    Loikkanen I. (2005) Polyamine homeostasis: Cellular responses to perturbation of polyamine biosynthetic enzymes. (Doctorate thesis), University of Oulu.Google Scholar
  16. 16.
    Martin-Tanguy J. (2001) Metabolism and function of polyamines in plants: recent development (new approaches). Plant Growth Regul. 34, 135–148.CrossRefGoogle Scholar
  17. 17.
    Mercedes, G., Manuel E. G., Angel J. M., Paloma M. R., Isabel M. S. (1994) Inhibition of polyamine synthesis by cyclohexylamine stimulates the ethylene pathway and accelerates the germination of Cicer arietinum seeds. Physiol. Plant. 91, 1–9.Google Scholar
  18. 18.
    Mirza J. I., Bagni N. (1991) Effect of exogenous polyamines and difluoromethylornithine on seed germination and root growth of Arabidopsis thaliana. Plant Growth Reg. 10, 163–168.CrossRefGoogle Scholar
  19. 19.
    Palavan N., Galston A. W. (1982) Polyamine biosynthesis and titer during various developmental stages of Phaseolus vulgaris. Physiol. Plantarum. 55, 438–444.CrossRefGoogle Scholar
  20. 20.
    Pohjanpelto P., Nordling S., Knuutila S. (1994) Flow cytometric analysis of the cell cycle in polyamine-depleted cells. Cytometry 16, 331–338.CrossRefGoogle Scholar
  21. 21.
    Rajam M. V. (1993) Polyamine biosynthesis inhibitors: New protectans against fungal plant disease. Curr Sci. 65, 461–469.Google Scholar
  22. 22.
    Sauve D. M., Anderson H. J., Ray J. M., James W. M., Roberge M. (1999) Phosphorylation-induced rearrangement of the histone H3 NH2-terminal domain during mitotic chromosome condensation. J. Cell Biol. 145, 225–235.CrossRefGoogle Scholar
  23. 23.
    Seidenfeld J., Gray J. W., Marton L. J. (1981) Depletion of 9L rat brain tumor cell polyamine content by treatment with D,L-alpha-difluoromethylornithine inhibits proliferation and the Gl to S transition. Exp. CellRes. 131, 209–216.CrossRefGoogle Scholar
  24. 24.
    Smith T. A. (1985) Polyamines. ANN Rev. Plant Physiol. 36, 117–143.CrossRefGoogle Scholar
  25. 25.
    Smith T. A., Best G. R. (1977) Polyamines in barley seedlings. Phytochemistry 16, 841–843.CrossRefGoogle Scholar
  26. 26.
    Soh Y. W., Yang, W. Y. (1993) Effect of plant growth regulators on mitotic chromosomes in Allium cepa L. Nucleus 36, 109–113.Google Scholar
  27. 27.
    Thomas T., Thomas T. J. (2001) Polyamines in cell growth and cell death: molecular mechanisms and therapeutic applications. Cell Mol. Life Sci. 58, 244–258.CrossRefGoogle Scholar
  28. 28.
    Tscherma-Woess E., Hasitschka G. (1953) Veranderungen der Kernstruktur wahrend der Endomitose, rhythmisches Kernwachstum und verschiedenes Heterochromatin bei Angiospermen. Chromosoma 5, 574–614.CrossRefGoogle Scholar
  29. 29.
    Unal M., Palavan-Unsal N., Tufekci M. A. (2002) Role of putrescine and its biosynthetic inhibitor on seed germination, root elongation and mitosis in Hordeum vulgare L. Bull. Pure App. Sci. 21B, 33–38.Google Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2008

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Meral Unal
    • 1
  • Narcin Palavan-Unsal
    • 2
  • M. A. Tufekci
    • 3
    Email author
  1. 1.Department of Biology, Arts and Sciences FacultyMarmara UniversityIstanbulTurkey
  2. 2.Faculty of Science and LettersIstanbul Kultur UniversityIstanbulTurkey
  3. 3.Department of Molecular Biology and Genetics, Faculty of Arts and SciencesTC Halic UniversityIstanbulTurkey

Personalised recommendations