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Acta Biologica Hungarica

, Volume 59, Issue 1, pp 17–29 | Cite as

Effects of Dexamethasone on Insulin Receptor in Aging

  • G. KoricanacEmail author
  • Mojca Stojiljkovic
  • Snezana Radivojsa
  • Zorica Zakula
  • Nevena Ribarac-Stepic
  • Esma R. Isenovic
Article

Abstract

The aim of this study was to examine the effects of dexamethasone (Dex) on functional properties of the rat insulin receptor (IR). Male Mill Hill hooded rats, 3, 6, 12, 18 and 21 months old, were injected with Dex (4 mg/kg) and rat liver and erythrocytes were used for experiments 18 h after Dex administration. Treatment with Dex lowered the specific binding (SB) of insulin (INS) in the liver of 3- and 18-month-old rats and concentration of INS binding sites (N1 N2) and the dissociation constant of low-affinity binding sites (Kd2) in the liver of 6- and 18-month-old rats. In addition, Dex treatment lowered the liver IR protein level in all analyzed groups, except 21-month-old rats where it remained unchanged, but raised the IR mRNA level in 18-month-old rats. In erythrocytes, treatment with Dex decreased SB and Kd2 (in animals 3 and 6 months old) and N] (in ones 3 and 18 months old). Following Dex treatment, the INS plasma level increased (in rats 3,18 and 21 months old), while glucose (Glu) concentration increased in 3 and 12 months old, but decreased in 6- and 21-month-old rats. In summary, Dex exerts the strongest effect on the erythrocyte IR of 3- and 6-month-old rats and the hepatic IR of 18-month-old rats. IR in both tissues is almost insensitive to Dex in 12- and 21-month-old rats. The pattern of age-related changes of IR induced by Dex does not correlate with changes of plasma Glu and INS.

Keywords

Dexamethasone insulin receptor aging liver erythrocytes 

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© Akadémiai Kiadó, Budapest 2008

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • G. Koricanac
    • 1
    Email author
  • Mojca Stojiljkovic
    • 1
  • Snezana Radivojsa
    • 1
  • Zorica Zakula
    • 1
  • Nevena Ribarac-Stepic
    • 1
  • Esma R. Isenovic
    • 2
  1. 1.Laboratory for Molecular Biology and Endocrinology“Vinca” Institute of Nuclear SciencesBelgradeSerbia
  2. 2.Laboratory for Radiobiology and Molecular Genetics“Vinca” Institute of Nuclear SciencesBelgradeSerbia

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