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Acta Biologica Hungarica

, Volume 58, Supplement 1, pp 51–64 | Cite as

Characterization of the APC10/DOC1 Subunit of the Anaphase Promoting Complex in Drosophila Melanogaster

  • Margit Pál
  • Kata Varga
  • Olga Nagy
  • P. DeákEmail author
Article

Abstract

The anaphase promoting complex or cyclosome (APC/C) is a large protein complex with an ubiquitin ligase activity which specifically targets mitotic regulatory proteins for proteasomal degradation. The APC/C contains at least 11 subunits, most of which are evolutionarily conserved from yeasts to humans. We have isolated and characterized mutant alleles of the gene that codes for the APClO/Docl subunit of the Drosophila APC/C. Loss of function APClO/Docl mutants have rudimentary imaginal discs and arrest their development as prepupae. Larval neuroblasts from these mutants show gross mitotic defects including high mitotic index, chromosome overcondensation, metaphase-like arrest and frequent aneu-ploid and polyploid cells. Mitotically arrested cells accumulate one of the main substrates of APC/C, cyclin B, most likely due to disabled ubiquitination activity. Our results suggest that the ApclO/Docl subunit has an essential role in establishing E3 ubiquitin ligase activity of APC/C in Drosophila.

Keywords

APC/C Ape 10/Doc 1 homologous recombination 

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© Akadémiai Kiadó, Budapest 2007

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Institute of Biochemistry, Biological Research CenterHungarian Academy of SciencesSzegedHungary

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