An All Optical Microfluidic Sorter


We present a microfluidic cell sorter that is able to count, characterize and sort micrometer sized particles and cells. In addition to optical counting and characterization, also sorting is performed by optical forces. The device is optimized for simplicity. The microfluidic channels and optical waveguides that carry the illuminating, detecting and sorting light form a single integrated structure, all built from the same material in a single photopolymerization step.


  1. 1.

    Applegate, Jr R. W., Squier, J., Vestad, T., David, J. O., Marr, W. M., Bado, R., Dugand, M. A., Saidd, A. A. (2006) Microfluidic sorting system based on optical waveguide integration and diode laser bar trapping. Lab-on-a-Chip 6, 422–426.

    CAS  Article  Google Scholar 

  2. 2.

    Ashkin, A., Dziedzic, J. M. (1987) Optical trapping and manipulation of viruses and bacteria. Science 235, 1517–1520.

    CAS  Article  Google Scholar 

  3. 3.

    Chena, C. C., Zappe, S., Sahin, O., Zhang, X. J., Fish, M., Scott, M, Solgaard, O. (2004) Design and operation of a microfluidic sorter for Drosophila embryos. Sensors and Actuators B102, 59–66.

    Google Scholar 

  4. 4.

    Cizmar, T., Siler, M., Sery, M., Zemanek, P., Garces-Chavez, V., Dholakia, K. (2006) Optical sorting and detection of submicrometer objects in a motional standing wave. Phys. Rev. B74, 035105.

    Article  Google Scholar 

  5. 5.

    Eriksson, E., Enger, J., Nordlander, B., Erjavec, N., Ramser, K., Goksör, M., Hohmann, S., Nyström, T., Hanstorp, D. (2007) A microfluidic system in combination with optical tweezers for analyzing rapid and reversible cytological alterations in single cells upon environmental changes. Lab-on-a-Chip (in press).

    Google Scholar 

  6. 6.

    Givan, A. L. (2001) Flow Cytometry-First Principles, 2nd ed., Wiley Liss, New York.

    Book  Google Scholar 

  7. 7.

    Hertzberg, R. P., Pope, A. J. (2004) High-throughput screening: New technology for the 21st century. Curr. Opin. Chem. Biol. 2000, 445.

    Google Scholar 

  8. 8.

    Juarez-Martinez, G., Steinmann, P., Roszak, A. W., Isaacs, N. W., Cooper, J. M. (2002) High-throughout screens for postgenomics: Studies of protein crystallization using microsystems technology. Anal. Chem. 74, 3505.

  9. 9.

    Kruger, J., Singh, K., O’Neill, A., Jackson, C, Morrison, A., O’Brien, P. (2002) Development of a microfluidic device for fluorescence activated cell sorting. J. Micromech. Microeng. 12, 486–494.

  10. 10.

    Lee, G.-B., Fu, L.-M., Yang, R. Y., Pan, Y.-J. (2003) Micro flow cytometers using electrokinetic forces with integrated optical fibers for on-line cell particle counting and sorting. Proc. 7th International Conference on Miniaturized Chemical and Biochemical Analysts Systems, Squaw Valley, California, USA.

    Google Scholar 

  11. 11.

    MacDonald, M. P., Splading, G. C., Dholakia, K. (2003) Microfluidic sorting in an optical lattice. Nature 426, 421.

    CAS  Article  Google Scholar 

  12. 12.

    Wang, Z., El-Ali, J., Perch-Nielsen, I. R., Mogensen, K. B., Snakenborg, D., Kutter, J. P., Wolff, A. (2004) Microchip Flow Cytometer with Integrated Polymer Optical Elements for Measurement of Scattered Light. Proc. IEEE MEMS pp. 710–713.

    Google Scholar 

  13. 13.

    Wang, M. M., Tu, E., Raymond, D. E., Yang, J. M., Zhang, Z., Hagen, N., Dees, B., Mercer, E. M., Forster, A. H., Kariv, I., Marchand, P. J., Butler, W. F. (2005) Microfluidic sorting of mammalian cells by optical force switching Nature Biotechnol. 23, 83–87.

    CAS  Article  Google Scholar 

  14. 14.

    Whitesides, G. M. (2006) The origins and the future of microfluidics. Nature 442, 368–373.

    CAS  Article  Google Scholar 

  15. 15.

    Wolff, A., Perch-Nielsen, I. R., Larsen, U. D., Friis, P., Goranovic, G., Poulsen, C. R., Kutter, J. P., Telleman, P. (2003) Integrating advanced functionality in a micro fabricated high-throughput fluorescent-activated cell sorter. Lab-on-a-Chip, 3, 22–27.

    CAS  Article  Google Scholar 

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Correspondence to P. Ormos.

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Kirei, H.I., Oroszi, L., Valkai, S. et al. An All Optical Microfluidic Sorter. BIOLOGIA FUTURA 58, 139–148 (2007).

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  • Cell particles
  • optical counting
  • FACS
  • photopolymerization