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Acta Biologica Hungarica

, Volume 58, Issue 4, pp 421–429 | Cite as

Cytological and Embryological Studies on Apospory in Bothriochloa ischaemum L.

  • G. H. MaEmail author
  • X. L. Huang
Article

Abstract

Cytological and embryological studies on apomictic species Bothriochloa ischaemum L. were carried out. Our studies revealed that the chromosome number of its root apical cells was 40, indicating that it was a tetraploid cytotype. During the stage of microsporogenesis, meiosis seemed irregular, as the pairing chromosome number of microspore mother cell was more than 20. It was often found that some chromosomes did not assemble in the equatorial plane or moved to the two poles of the cell, a few laggards were seen. Multiporate pollens (22.3%) were often observed. The studies showed that a high frequency (87.8%) of 1–3 or more aposporous embryo sacs developed in one ovule of the species. The mature aposporous sac was usually characterized by an egg cell and one polar nucleus. The egg cell could develop spontaneously into a large proembryo (100–200 μm) mass prior to anthesis. When several aposporous sacs occurred in the same ovule, usually 2 aposporous sacs were involved in pseudogamy and developed into separate endosperm masses in the same ovary. In the low frequency of mature seed, 13.5% twin-embryo seedlings could be obtained after mature seeds germinated.

Keywords

Bothriochloa ischaemum L. apospory pseudogamy cytology embryology 

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Notes

Acknowledgements

The support by Program of Guangdong Digital Botanical Garden and the Tropical and Subtropical Plant Germplasm Construction in Guangdong Province 2005B60301001 is greatly acknowledged and article has been reviewed by an Australia scientist Dr. Eric Bunn in Kings Park and Botanic Garden was also acknowledged.

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© Akadémiai Kiadó, Budapest 2007

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.South China Botanical GardenThe Chinese Academy of SciencesGuangzhouChina
  2. 2.College of Life SciencesZhongshan UniversityGuangzhouChina

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