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Acta Biologica Hungarica

, Volume 58, Issue 4, pp 397–409 | Cite as

Anti-Genotoxic Effect of Ocimum sanctum L. Extract against Cyproterone Acetate Induced Genotoxic Damage in Cultured Mammalian Cells

  • Y. H. SiddiqueEmail author
  • Gulshan Ara
  • T. Beg
  • M. Afzal
Article

Abstract

The anti-genotoxic effect of Ocimum sanctum L. extract was studied against the genotoxic effect induced by a synthetic progestin cyproterone acetate, on human lymphocytes using chromosomal aberrations, mitotic index, sister chromatid exchanges and replication index as a parameters. About 30 μM of cyproterone acetate was treated with O. sanctum L. infusion, at dosages of 1.075 × 10−4, 2.125 × 10−4 and 3.15 × 10−4 g/ml of culture medium. A clear dose-dependent decrease in the genotoxic damage of cyproterone acetate was observed, suggesting a possible modulating role of the plant infusion. The results of the present study suggest that the plant infusion per se does not have genotoxic potential, but can modulate the genotoxicity of cyproterone acetate on human lymphocytes in vitro.

Keywords

Ocimum sanctum cyproterone acetate anti-genotoxicity human lymphocytes aqueous extract 

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Notes

Acknowledgements

Thanks are due to the CSIR, New Delhi for awarding the Fellowship No. 9/112(353)/2003-EMR to the author (YHS) and to the Chairman, Department of Zoology, A.M.U., Aligarh, U.P., for laboratory facilities.

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© Akadémiai Kiadó, Budapest 2007

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Y. H. Siddique
    • 1
    Email author
  • Gulshan Ara
    • 1
  • T. Beg
    • 1
  • M. Afzal
    • 1
  1. 1.Human Genetics and Toxicology Laboratory, Section of Genetics, Department of Zoology, Faculty of Life SciencesAligarh Muslim UniversityAligarhIndia

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