Copper-Induced Oxidative Stress in Maize Shoots (Zea Mays L.): H2O2 Accumulation and Peroxidases Modulation

Abstract

The effect of copper excess on growth, H2O2 level and peroxidase activities were studied in maize shoots. Ten-day-old seedlings were cultured in nutrient solution that contained Cu2+ ions at various concentrations (50 and 100 μM) for seven days. High concentrations of Cu2+ ions caused significant decrease both in matter production and elongation of maize shoots. In addition, treatment with CuSO4 increased levels of H2O2 and induced changes in several peroxidase activities. Moreover, the disturbance of the physiological parameters was accompanied by the modulation of the peroxidase activities: GPX (Guaiacol peroxidase, EC 1.11.1.7), CAPX (Coniferyl alcohol peroxidase, EC 1.11.1.4) and APX (Ascorbate peroxidase, EC.1.11.1.11). Furthermore, this modulation becomes highly significant, especially, in the presence of 100 μM of CuSO4.

References

  1. 1.

    Alloway, B. J. (1995) Heavy Metals in Soils. Second Edition, Blackie Academic and Professional, London.

    Book  Google Scholar 

  2. 2.

    Alvarez, M. E., Penel, R. I., Meijer, P. J., Ishikaw, A., Dixon, R. A., Lamb, C. (1998) Reactive oxygen intermediate a systemic signal network in the establishment of plant immunity. Cell 92, 773–784.

    CAS  Article  Google Scholar 

  3. 3.

    Baccouche, S., Chaoui, A., El Ferjani, E. (1998) Nickel induced oxidative damage and antioxidant responses in Zea mays shoots. Plant Physiol. Biochem. 36, 6896–6898.

    Google Scholar 

  4. 4.

    Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principale of protein-dye binding. Anal. Biochem. 72, 248–258.

    CAS  Article  Google Scholar 

  5. 5.

    Chen, E. L., Chen, Y. A., Chen, L. M., Liu, Z. H. (2002) Effect of copper on peroxidase activity and lignin content in Raphanus sativus. Plant Physiol. Biochem. 40, 439–444.

    CAS  Article  Google Scholar 

  6. 6.

    Cuypers, A., Vangronsveld, J., Clijsters, H. (2000) Biphasic effect of copper on the ascorbate-glutathione pathway in primary leaves of Phaseolus vulgaris seedlings during early stages of metal assimilation. Physiol. Plant. 110, 512–517.

    CAS  Article  Google Scholar 

  7. 7.

    Elstner, E. F., Wagner, G. A., Schütz, W. (1988) Activated oxygen in green plants in relation to stress situations. Curr. Topics Plant Biochem. 36, 873–877.

    Google Scholar 

  8. 8.

    Fielding, J. L., Hall, J. L. (1978) A biochemical and cytochemical study of peroxidase activity in roots of Pisum sativum. J. Exp. Bot. 29, 979–986.

    Google Scholar 

  9. 9.

    Gabbrielli, R. M., Pandolfini, T. M., Vergano, O., Palandri, M. R. (1990) Comparison of two serpentine species with different nickel tolerance strategies. Plant Soil 122, 271–277.

    CAS  Article  Google Scholar 

  10. 10.

    Gupta, M., Cuypers, A., Vangronsveld, J., Clijsters, H. (1999) Copper affects the enzymes of the ascorbate-glutathione cycle and its related metabolites in roots of Phaseolus vulgaris. Physiol Plant. 106, 262–267.

    CAS  Article  Google Scholar 

  11. 11.

    Halliwell, B., Gutteridge, J. M. C. (1984) Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem. J. 219, 1–14.

    CAS  Article  Google Scholar 

  12. 12.

    Jouili, H., El Ferjani, E. (2003) Changes in antioxidant and lignifying enzyme activities in sunflower roots (Helianthus annuus L.) stressed with copper excess. C. R. Biologies 326, 639–644.

    CAS  Article  Google Scholar 

  13. 13.

    Jouili, H., El Ferjani, E. (2004) Effect of copper excess on superoxide dismutase, catalase, and per-oxidase activities in sunflower seedlings (Helianthus annuus L.). Acta physiologiae Plantarum 26, 29–35.

    CAS  Article  Google Scholar 

  14. 14.

    Lin, C. C., Chen, L. M., Liu, Z. H. (2005) Rapid effect of copper on lignin biosynthesis in soybean roots. Plant Sci. 168, 858–861.

    Google Scholar 

  15. 15.

    Mazhoudi, S., Chaoui, A., Gorbal, M. H., El Ferjani, E. (1997) Response of antioxidant enzymes to excess copper in tomato (Lycopersion esculentum, Mill.). Plant Sci. 127, 182–186.

    Article  Google Scholar 

  16. 16.

    Mocquot, B., Vangronseveld, J., Clijsters, H., Mench, M. (1996) Copper toxicity in young maize (Zea mays L.) plants: effects on growth, mineral and chlorophyll contents and enzyme activities. Plant and Soil 182, 287–300.

    CAS  Article  Google Scholar 

  17. 17.

    Nakano, Y., Asada, K. (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplast. Plant Cell Physiol. 22, 423–430.

    Google Scholar 

  18. 18.

    Pandolfini, T., Gabbrielli, R., Comparini, C. (1992) Nickel toxicity and peroxidase activity in seedlings of Triticum aestivum L. Plant Cell Environ. 15, 719–725.

    CAS  Article  Google Scholar 

  19. 19.

    Pedereńo, M. A., Barcelo, A. R., Sabatter, F., Munoz, R. (1989) Control by pH of cell wall peroxi-dase activity involved in lignification. Plant Cell Physiol. 30, 237–241.

    Article  Google Scholar 

  20. 20.

    Quiroga, M., Guerrero, C., Botella, M. A., Barceló, Amaya, I., Medina, M. I., Alonso, F. J., Forchetti, S. M., Tigier, H., Valpuesta, V. (2000) A tomato peroxidase involved in the synthesis of lignin and suberin. Plant Physiol. 122, 1119–1127.

    CAS  Article  Google Scholar 

  21. 21.

    Sandmann, G., Böger, P. (1980) Copper-mediated lipid peroxidation processes in photosynthetic membranes. Plant Physiol. 66, 797–800.

    CAS  Article  Google Scholar 

  22. 22.

    Sanita Di Troppi, L., Gabbrielli, R. (1999) Response to cadmium in higher plants. Environ. Exp. Bot. 41, 105–130.

    Article  Google Scholar 

  23. 23.

    Scarponil, L., Perucci, P. (1984) Effects of some metals and related metal organic compounds on ALA-deshydratase activity of corn. Plant and Soil 79, 69–75.

    Article  Google Scholar 

  24. 24.

    Schützendübel, A., Nikolova, I. P., Rudolf, C., Polle, A. (2002) Cadmium and H2O2-induced oxidative stress in Populus×canescens roots. Plant Physiol. Biochem. 40, 577–584.

    Article  Google Scholar 

  25. 25.

    Sergiev, I., Alexieva, V., Karanov, E. (1997) Effect of spermine, atrazine and combination between them on some endogenous protective systems and stress markers in plants. Comt. Rend. Acad. Bulg. Sci. 51, 121–124.

    Google Scholar 

  26. 26.

    Sgherri, C., Milone, M. T. A., Clijsters, H., Navari-Izzo, F. (2001) Antioxidative enzymes in two wheat cultivars, differently sensitive to drought and subjected to subsymptomatic copper doses. J. Plant Physiol. 158, 1439–1447.

    CAS  Article  Google Scholar 

  27. 27.

    Shigeoka, S., Ishikawa, T., Tamoi, M., Miyagawa, Y., Takeda, T., Yabuta, Y. R., Kazuya, Y. (2002) Regulation and function of ascorbate peroxidase isoenzymes. J. Exp. Bot. 53, 1305–1319.

    CAS  Article  Google Scholar 

  28. 28.

    Van Assche, F., Clijsters, H. (1990) Effects of metals on enzyme activity in plants. Plant Cell Environ. 13, 195–206.

    Article  Google Scholar 

  29. 29.

    Velikova, V., Yordanov, I., Edreva, Q. (2000) Oxidative and some antioxidant systems in acid rain-treated bean plants. Protection role of exogenous polyamines. Plant Science 151, 59–66.

    CAS  Article  Google Scholar 

  30. 30.

    Wainwright, S. J., Woolhouse, H. W. (1977) Some physiological aspects of copper and zinc tolerance in Agrostis tenius sibth: cell elongation and membrane damage. J. Exp. Bot. 281, 1029–1036.

    Article  Google Scholar 

  31. 31.

    Yruela, I. (2005) Copper in plants. Braz. J. Plant Physiol. 171, 145–156.

    Article  Google Scholar 

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Correspondence to E. El Ferjani.

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Bouazizi, H., Jouili, H. & El Ferjani, E. Copper-Induced Oxidative Stress in Maize Shoots (Zea Mays L.): H2O2 Accumulation and Peroxidases Modulation. BIOLOGIA FUTURA 58, 209–218 (2007). https://doi.org/10.1556/ABiol.58.2007.2.7

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Keywords

  • Peroxidases
  • copper
  • H2O2
  • Zea mays L.