Acetylsalicylic Acid Decreases the Labeling of Blood Constituents with Technetium-99m
Acetylsalicylic acid is the most widely used drug as antipyretic, analgesic, anti-inflammatory agent and for secondary prevention of thrombotic phenomena in the heart, brain and peripheral circulation. Drugs can modify the labeling of blood constituents with technetium-99m (99mTc). This work has evaluated the effect of in vivo treatment with acetylsalicylic acid on the in vitro labeling of the blood constituents with 99mTc. Wistar rats were treated with different doses (1.5, 3.0 and 6.0 mg/kg) of acetylsalicylic acid during 1 hour. At higher dose used (6.0 mg/kg) animals were treated during different period of time (0.25, 1.0 and 4.0 hours). Animals treated with physiologic saline solution were used as control. After the labeled process; plasma (P), blood cells (BC), insoluble (IF-P, IF-BC) and soluble (SF-P, SF-BC) fractions were separated. Afterwards, the percentage of radioactivity (%ATI) in each fraction was calculated. The treatment during 1 hour with acetylsalicylic acid at higher dose has significantly (p < 0.05) modified the fixation of 99mTc on blood cells. Considering the results, we suggest that acetylsalicylic acid used at therapeutic doses may interfere with the nuclear medicine procedures related to these blood constituents.
KeywordsAcetylsalicylic acid blood constituents technetium-99m
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- 1.Abreu, P. R., Almeida, M. C., Bernardo, R. M., Bernardo, L. C., Brito, L. C., Garcia, E. A., Fonseca, A. S., Bernardo-Filho, M. (2006) Guava extract (Psidium guajava) alters the labelling of blood constituents with technetium-99m. J. Zhejiang Univ. Sci. B 7, 429–435.PubMedPubMedCentralGoogle Scholar
- 2.Antithrombotic Trialists’Collaboration, meta-analysis of randomised trials of antiplatelet therapy for prevention of death, myocardial infarction, and stroke in high risk patients. (2002) Br. Med. J. 324, 71–86.Google Scholar
- 3.Aude, Y. W., Mehta, J. L. (2002) Nonplatelet-mediated effects of aspirin. Drugs Today (Barc) 38, 501–507.Google Scholar
- 4.Baker, W. J., Datz, F. L. (1987) Preparation and clinical utility of labeled blood products. In: Saha, G. B. (ed.) Essentials of Nuclear Medicine Sciences. Williams and Wilkins, Sydney, pp. 90–98.Google Scholar
- 5.Bernardo-Filho, M., Gutfilen, B., Maciel, O. S. (1994) Technetium-99m binding on plasma proteins and red blood cells: role of various precipitating agents. Biomed. Lett. 50, 17–24.Google Scholar
- 6.Bernardo-Filho, M., Moura, I. N. S., Boasquevisque, E. M. (1983) 99mTechnetium-labeled red blood cells “in vitro”. Arch. Biol. Technol. 26, 455–461.Google Scholar
- 9.Catella-Lawson, F. (2001) Vascular biology of thrombosis: platelet-vessel wall interactions and aspirin effects. Neurology 57 (supp 2), 5-7.Google Scholar
- 11.Christioan, T. F., Milavetz, J. J., Miller, T. D., Clements, I. P., Holmes, D. R. Gibbons, R. J. (1998) Prevalence of spontaneous reperfusion and associated myocardial salvage in patients with acute myocardial infarction. Am. Heart J. 135, 421–427.Google Scholar
- 15.Dewanjee, M. K., Rao, S. A., Penniston, J. T. (1982) Mechanism of red blood cell labeling with 99mTechnetium-pertecnetate and the role of cation pumps at RBC membrane on distribution and binding of Sn+2 and 99mTechnetium with membrane proteins and hemoglobin. J. Labelled Compd. Radiopharm. 11, 1464–1466.Google Scholar
- 18.Etminan, M., Gill, S., Samii, A. (2003) Effect of non-steroidal anti-inflammatory drugs on risk of Alzheimer’s disease: systematic review and meta-analysis of observational studies. BMJ 327(7407), 128.Google Scholar
- 20.Frydman, J. N. G., Oliveira, M. B. N., Santos, A. E. O., Fonseca, A. S., Santos, R., Bernardo-Filho, M. (2004) Influence of methylxanthines on the labeling of blood elements with 99mTechnetium. Pak. J. Biol. Sci. 4, 521–524.Google Scholar
- 22.Guerrero, A., González-Correa, J. A., Arrebola, M. M., Muñoz-Marín, J., Sánchez de La Cuesta, F., De La Cruz, J. P. (2004) Antioxidant effects of a single dose of acetylsalicylic acidand salicylic acid in rat brain slices subjected to oxygen-glucose deprivation in relation with its antiplatelet effect. Neurosci. Lett. 328, 153–156.Google Scholar
- 23.Gutfilen, B., Boasquevisque, E. M., Bernardo-Filho, M. (1992) Calcium channel blockers: interference on red blood cells and plasma proteins labeling with 99mTc. Rev. Esp. Med. Nucl. 11, 195–199.Google Scholar
- 24.Harel, F., Dupuis, J., Benelfassi, A., Ruel, N., Gregoire, J. (2005) Radionuclide plethysmography for non-invasive evaluation of peripheral arterial blood flow. Am. J. Physiol. Heart Circ. Physiol. 289, 258–262.Google Scholar
- 25.Insel, P. A. (2001) Analgesic-antipyretic and antiinflammatory agents and drugs employed in the treatment of gout. In: Hardman, J. G., Limbird, L. E., Gilman, A. G. (eds) The Pharmacological Basis of Therapeutics. 10th McGraw-Hill, New York, pp. 617–657.Google Scholar
- 29.Langman, M. J. (1994) Adverse effects of conventional non-steroidal anti-inflammatory drugs on the upper gastrointestinal tract. Fundam. Clin. Pharmacol. 17, 393–403.Google Scholar
- 30.Moore, G. J. (1985) Kinetics of acetylation-deacetylation of angiotensin II. Int. J. Peptide Protein Res. 26, 469–481.Google Scholar
- 35.Schwenger, P., Bellosta, P., Vietor, I., Basilico, C., Skolnik, E. Y., Vilcek, J. (1997) Sodium salicylate induces apoptosis via p38 mitogen-activated protein kinase but inhibits tumor necrosis factor-induced c-Jun N-terminal kinase/stress-activated protein kinase activation. Proc. Natl. Acad. Sci. USA 94, 2869–2873.PubMedGoogle Scholar
- 41.Weissmann, G. (1992) NSAIDs: aspirin and aspirin-like drugs. In: Wyngaarden, J. B., Smith, L. H. Jr., Benneth, J. C. (eds) Cecil Textbook of Medicine. 19th Edition. Saunders, Philadelphia, p. 114.Google Scholar
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