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Acta Biologica Hungarica

, Volume 58, Issue 2, pp 173–185 | Cite as

Myocardial Antioxidant Status and Oxidative Stress after Combined Action of Exercise Training and Ethanol in Two Different Age Groups of Male Albino Rats

  • K. Pushpalatha
  • K. Nishanth
  • K. Sathyavelu ReddyEmail author
Article

Abstract

The interaction of exercise training and ethanol on the myocardial antioxidant enzymes and the oxida-tive stress markers was investigated in the Wistar strain male albino rats. We also tested the interactive effects of exercise training and ethanol on the age-associated free radical production and antioxidant defense system. We found a significant decrease (p <0.05) in the activity levels of superoxide dismutase (SOD) and catalase (CAT) in the myocardium of old rats when compared to young rats by 26% and 58%, respectively, suggesting the onset of age-dependent decrease in the myocardial antioxidant enzyme system. In contrast to the decreased antioxidant enzyme activity, xanthine oxidase (XOD) and lipid peroxidation (LPO) levels were elevated, suggesting the age-induced oxidative stress. Exercise training significantly (p < 0.05) elevated the activities of SOD, CAT, XOD and LPO levels in both the age groups of animals. Ethanol consumption significantly lowered the SOD and C AT activities in both the age groups, whereas a significant increase was observed in the XOD and LPO levels. In contrast, the combination of exercise training plus ethanol lowered XOD and LPO levels in both the age groups of rats compared to ethanol treated rats. A significant (p < 0.05) increase in the activities of SOD and CAT was reported in the rats treated with the combination of exercise training plus ethanol. This increase was more pronounced in the younger rats than the older rats. The findings of the present investigation on the potential role of antioxidant enzymes to counter the ethanol-induced pro-oxidants showed an increase with the interaction of exercise training. With age, a decrease in the antioxidant enzyme capacity was observed. This reveals that the old age rats were more affected to the pro-oxidants when compared to the young age rats. In conclusion it is demonstrated that two months treadmill endurance exercise training is beneficial to both young and old rats in improving antioxidant defense to challenge the oxidative stress in the myocardial tissue and thereby successfully countering the free radical production due to ethanol intoxication.

Keywords

Aging exercise training ethanol oxidative stress antioxidant defense system 

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Notes

Acknowledgements

Grant sponsor: University Grants Commission (UGC), New Delhi (Project No. MRP-F.3-125/2001 (SR-II) dated 29-3-2001) to Dr. K. Sathyavelu Reddy.

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© Akadémiai Kiadó, Budapest 2007

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • K. Pushpalatha
    • 1
  • K. Nishanth
    • 2
  • K. Sathyavelu Reddy
    • 1
    Email author
  1. 1.Department of Zoology, Division of Exercise PhysiologySri Venkateswara UniversityTirupatiIndia
  2. 2.Department of BiotechnologySree Vidyaniketan Engineering CollegeA. RangampetaIndia

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