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Acta Biologica Hungarica

, Volume 57, Issue 4, pp 459–471 | Cite as

Amplified Fragment Length Polymorphism (AFLP) is Useful for Finding Markers Associated with QTL for Architectural Trait in Hedysarum coronarium L.

  • S. Marghali
  • H. Chennaoui
  • H. Bourguiba
  • M. Marrakchi
  • N. Trifi-FarahEmail author
Open Access
Article

Abstract

Amplified fragment length polymorphism (AFLP) has been proposed as a valuable tool for finding molecular markers linked to QTL controlling architectural trait. Segregation of polymorphic AFLP fragments was followed in F2 offsprings Hedysarum coronarium derived from two native wild accesions crossing: Jebel Zit × El Haouaria, which had previously characterised by morphological and AFLP markers. A comparison of profiles from the morphologically contrasted parental individuals and F2 offspring has been assessed using five AFLP primer combinations. These generated 178 bands revealed by silver-stained denaturing polyacrylamide gels, of which 150 were polymorphic. Several AFLP markers appeared to be implied in the orthotropic form that can assist the selection and improve Hedysarum forage crop.

Keywords

AFLP markers QTL association architecture Hedysarum 

Notes

Acknowledgements

This research was supported by grants in Tunisia from the “Ministère de la Recherche Scientifique, de la Technologie et du Développement des Compétences”. We are grateful to Pr Rebai A (CB Sfax, Tunisia) for his fruitful help.

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© Akadémiai Kiadó, Budapest 2006

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • S. Marghali
    • 1
  • H. Chennaoui
    • 1
  • H. Bourguiba
    • 1
  • M. Marrakchi
    • 1
  • N. Trifi-Farah
    • 1
    Email author
  1. 1.Laboratoire de Génétique Moléculaire, Immunologie et BiotechnologieFaculté des Sciences de Tunis, Campus universitaireEl Manar, TunisTunisia

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