Amplified Fragment Length Polymorphism (AFLP) is Useful for Finding Markers Associated with QTL for Architectural Trait in Hedysarum coronarium L.

Abstract

Amplified fragment length polymorphism (AFLP) has been proposed as a valuable tool for finding molecular markers linked to QTL controlling architectural trait. Segregation of polymorphic AFLP fragments was followed in F2 offsprings Hedysarum coronarium derived from two native wild accesions crossing: Jebel Zit × El Haouaria, which had previously characterised by morphological and AFLP markers. A comparison of profiles from the morphologically contrasted parental individuals and F2 offspring has been assessed using five AFLP primer combinations. These generated 178 bands revealed by silver-stained denaturing polyacrylamide gels, of which 150 were polymorphic. Several AFLP markers appeared to be implied in the orthotropic form that can assist the selection and improve Hedysarum forage crop.

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Acknowledgements

This research was supported by grants in Tunisia from the “Ministère de la Recherche Scientifique, de la Technologie et du Développement des Compétences”. We are grateful to Pr Rebai A (CB Sfax, Tunisia) for his fruitful help.

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Correspondence to N. Trifi-Farah.

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Marghali, S., Chennaoui, H., Bourguiba, H. et al. Amplified Fragment Length Polymorphism (AFLP) is Useful for Finding Markers Associated with QTL for Architectural Trait in Hedysarum coronarium L.. BIOLOGIA FUTURA 57, 459–471 (2006). https://doi.org/10.1556/ABiol.57.2006.4.8

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Keywords

  • AFLP markers
  • QTL
  • association
  • architecture
  • Hedysarum