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Acta Biologica Hungarica

, Volume 57, Issue 3, pp 355–365 | Cite as

Protection Against Salt Toxicity in Azolla pinnata-Anabaena azollae Symbiotic Association by Using Combined-N Sources

  • A. K. Mishra
  • Satya S. Singh
Article

Abstract

Protection from salt stress was observed in the terms of yield (fresh and dry weight, chlorophyll and protein) and nitrogenase activity. Azolla pinnata appeared highly sensitive to 40 mM external NaCl stress. Fronds of Azolla unable to grow beyond a concentration of 30 mM NaCl and accordingly death was recorded at 40 mM NaCl on the 6th day of incubation. Yield was inhibited by various levels of NaCl (0, 10, 20 and 30 mM). Addition of combined-N to the growth medium protected the association partially from salt toxicity. Among the N-sources (NO3, NH4+ and urea) tried, urea mitigated the salt-induced toxicity most efficiently. Reduction in nitrogenase activity was observed when intact Azolla was grown in nutrient medium either supplemented with different levels of NaCl or combined nitrogen. Only NO3 (5 mM) protected the enzymatic activity from salt toxicity while other concentrations of ammonium, nitrate and urea slowed down the salt-induced inhibition of enzyme activity in Azolla-Anabaena association. These results suggested that an optimum protection from salt stress could be obtained by using a combination of combined nitrogen sources. The reason for this protection might be due to the availability of combined nitrogen to the association, nitrogen is only available through the biological nitrogen fixation which is the most sensitive to salt stress.

Keywords

Azolla pinnata combined-N growth nitrogenase salinity 

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Notes

Acknowledgements

Dr. Satya S. Singh is thankful to CSIR, New Delhi for financial assistance. Thanks are also due to Head, Department of Botany for providing laboratory facilities.

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© Akadémiai Kiadó, Budapest 2006

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • A. K. Mishra
    • 1
  • Satya S. Singh
    • 1
  1. 1.Department of BotanyBanaras Hindu UniversityVaranasiIndia

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