Advertisement

Acta Biologica Hungarica

, Volume 57, Issue 3, pp 301–313 | Cite as

Investigation of Long-Term Stress Induced by Several Stressors by Determination of the Concentration of Different Blood Plasma Components in a Model of Prussian Carp (Carassius auratus gibelio Bloch, 1783) and Common Carp (Cyprinus carpio L., 1758)

  • Á. HegyiEmail author
  • T. Béres
  • L. Váradi
  • K. K. Lefler
  • B. Tóth
  • B. Urbányi
Article

Abstract

Several compounds (carbohydrates, proteins, hormones, etc.) were used in fish to quantify the level of stress. Our investigations focused on two parameters of the blood plasma: plasma glucose and serum/plasma fructosamine (SeFa) that has not been tested on fish as yet. Experiments were conducted on two fish species. The concentrations of these components were investigated on Common carp (Cyprinus carpio L., 1758) and on Prussian carp (Carassius auratus gibelio Bloch, 1783) from the Gödöllő-Isaszeg pond system by creating conditions different from ideal. Stress effects caused a fluctuating tendency in blood plasma glucose levels each week for both Common carp and Prussian carp, thus, there was no steady growth. However, SeFa concentrations exactly followed stress effects, moreover, it tolerated short-term negative effects (handling of fish, blood sampling) and did not cause alterations at individuals blood samplings. This experimental method can offer assistance to farmers in the daily routine (e.g. in fish transport) and in the technology of propagation.

Keywords

Stress serum/plasma fructosamine plasma glucose Common carp Prussian carp 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Notes

Acknowledgements

The authors thank Ferenc Imre, Károly Oláh (Béke Anglers’ Association) and Krisztián Szabó (Dinnyés Fish Hatchery) for the experimental fish stocks.

References

  1. 1.
    Barcellos, L. J. G., Kreutz, L. C., Quevedo, R. M., Fioreze, I., Cericato, L., Soso, A. B., Fagundes, M., Conrad, J., Baldissera, R. K., Bruschi, A., Ritter, F. (2004) Nursery rearing of jundia, Rhamdia quelen (Quoy & Gaimard) in cages: cage type, stocking density and stress response to confinement. Aquaculture 232, 383–394.CrossRefGoogle Scholar
  2. 2.
    Clement, T. S., Parikh, V., Schrumpf, M., Fernald, R. D. (2005) Behavioral coping strategies in a cichild fish: the role of social status and acute stress response in direct and displaced aggression. Horm. Beh. 47, 336–342.CrossRefGoogle Scholar
  3. 3.
    Colombo, L., Pickering, A. D., Belvedere, P., Schreck, C. B. (1990) Stress inducing factors and stress reaction in aquaculture. In: N. De Pauw and R. Billard (eds) Aquaculture Europe ’89 — Business Joins Science. European Aquaculture Society, Special Publ. No. 12. Bredene, Belgium. 93–121.Google Scholar
  4. 4.
    Fernald, R. D. (2003) How does behavior change the brain? Multiple methods to answer old questions. Integ. Comp. Biol. 43, 771–779.CrossRefGoogle Scholar
  5. 5.
    Gensic, M., Wissing, P. J., Keefe, T. R., Mustafa, A. (2004) Effects of iodized on stress modulation in steelhead trout, Oncorhynchus mykiss (Walbaum). Aquac. Res. 35, 1117–1121.CrossRefGoogle Scholar
  6. 6.
    Gravel, A., Campbell, P. G. C., Hontela, A. (2005) Disruption of the hypothalamo-pituitary-interrenal axis in 1+ yellow perch (Perca flavescens) chronically exposed to metals in the environment. Can. J. Fish. Aquat. Sci. 62, 982–990.CrossRefGoogle Scholar
  7. 7.
    Gutierrez-Estrada, J. C., de Pedro-Sanz, E., Lopez-Luque, R., Pulido-Calvo, I. (2004) Comparison between traditional methods and artificial nueral networks for ammonia concentration forecasting in an eel (Anguilla anguilla L.) intensive rearing system. Aquacult. Eng. 31, 183–203.CrossRefGoogle Scholar
  8. 8.
    Henrique, M. M. F., Gouillou-Coustans, M. F., Gomes, E. (2002) Effect of dietary ascorbic acid supplementation and chronic hypoxia on sea bream growth and vitamin C status. J. Fish Biol. 60, 442–452.CrossRefGoogle Scholar
  9. 9.
    Hoeger, B., van den Heuvel, M. R., Hitzfeld, B. C., Dietrich, D. R. (2004) Effect of treated sewage effluent on immune function in rainbow trout (Oncorhyncus mykiss). Aquat. Toxicol. 70, 345–355.CrossRefGoogle Scholar
  10. 10.
    Jurvelius, J., Riikonen, R., Marjomaki, T. J., Lilja, J. (2000) Mortality of pike-perch (Stizostedion lucioperca), brown trout (Salmo trutta) and landlocked salmon (Salmo salar m. sebago) caught as bycatch in pelagic trawling in a Finnish lake. Fish. Res. 45, 291–296.CrossRefGoogle Scholar
  11. 11.
    Lyytikainen, T., Pylkko, P., Ritola, O., Lindstrom-Seppa, P. (2002) The effect of acute stress and temperature on plasma cortisol and ion concentration and growth of Like Inari Arctic charr, Salvenius alpinus. Environ. Biol. Fish. 64, 195–202.CrossRefGoogle Scholar
  12. 12.
    Olsen, R. E., Sundell, K., Hansen, T., Hemre, G. I., Myklebust, R., Mayhew, T. M., Ringo, E. (2002) Acute stress alters the intestinal lining of Atlantic salmon, Salmo salar L.: An electron microscopical study. Fish. Physiol. Biochem. 26, 211–221.CrossRefGoogle Scholar
  13. 13.
    Oppel, K., Kulcsár, M., Bárdos, L., Ferencz, A., Lakner, H., Simon, J., Temesváry, K., Karchesz, K. (2000a) A new, modern, cost-saving micro/macro method for the determination of serum frutosamine. Acta Vet. Hung. 48, 285–291.CrossRefGoogle Scholar
  14. 14.
    Oppel, K., Bárdos, L., Lakner, H., Temesváry, K., Bölcsházy, G., Kulcsár, M., Ferencz, A., Simon, J. (2000b) Glucosylated (glycated) proteins and the importance of their investigation in domestic animals: Literature rewiev (in Hungarian, with English abstract). Magy. Állatorv. Lapj. 122, 106–111.Google Scholar
  15. 15.
    Pierson, P. M., Lamers, A., Flik, G., Mayer-Gostan, N. (2004) The stress axis, stanniocalcin, and ion balance in rainbow trout. Gen. Comp. Endocr. 137, 263–271.CrossRefGoogle Scholar
  16. 16.
    Phillips, T. A., Summerfelt, R. C., Atchison, G. J. (2002) Environmental biological, and methodological factors affecting cholinesterase activity in walleye (Sitzostedion vitreum). Arch. Environ. Con. Tox. 43, 75–80.CrossRefGoogle Scholar
  17. 17.
    Plante, S., Audet, C., Lambert, Y., de la Noue, J. (2003) Comparison of stress responses in wild and captive winter flounder (Pseudopleuronectes americanus Walbaum) broodstock. Aquac. Res. 34, 803–812.CrossRefGoogle Scholar
  18. 18.
    Shrimpton, J. M., Zydlewski, J. D., McCormick, S. D. (2001) The stress response of juvenile American shad to handling and confinement is greater duting migration in freshwater than in seawa-ter. Trans. Am. Fish. Soc. 130, 1203–1210.CrossRefGoogle Scholar
  19. 19.
    Wingfield, J. C., Sapolsky, R. M. (2003) Reproduction and resistance to stress: When and how. J. Neuroendocrinol. 15, 711–724.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2006

Authors and Affiliations

  • Á. Hegyi
    • 1
    Email author
  • T. Béres
    • 1
  • L. Váradi
    • 1
  • K. K. Lefler
    • 1
  • B. Tóth
    • 2
  • B. Urbányi
    • 1
  1. 1.Department of Fish Culture, Faculty of Agricultural and Environmental SciencesSzent István University, Páter Károly u. 1GödöllőHungary
  2. 2.Duna-Ipoly National Park DirectorateBudapestHungary

Personalised recommendations