Identification of Adsorption Inhibition, Restriction/Modification and Abortive Infection Type Phage Resistance Systems in Lactococcus lactis Strains
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Abstract
98 Lactococcus lactis strains were isolated from traditional fermented milk products in Turkey tested against 60 lactococcal lytic phages to determine their resistance levels. While 82 L. lactis strains were sensitive against lactic phages at different levels, 16 L. lactis strains showed resistance to all phages tested. Types of phage resistance among 16 L. lactis strains were identified as phage adsorption inhibition in eight strains, restriction/modification in six strains and abortive infection (heat sensitive phage resistance) in two strains, using three broad-spectrum phages Φpll 98-32, Φpld 67-42 and Φpld 67-44.
Keywords
L. lactis phage resistance adsorption restriction/modification abortive infectionPreview
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Acknowledgements
This research was supported by the Institute of Biotechnology, Ankara University, under the project entitled “Endüstriyel Açıdan Kullanışlı Lactococcus lactis Suşlarının Tanısıve Laktik Fajların İnaktivasyonu Üzerinde Araştırmalar”.
References
- 1.Akçelik, M. (1998) A phage DNA injection-blocking type resistance mechanisms encoded by chromosomal DNA in Lactococcus lactis subsp. lactis PLM18. Milchwissenchaft 53, 619–622.Google Scholar
- 2.Akçelik, M., Şanlibaba, P., Tükel, Ç. (2000) Phage resistance in Lactococcus lactis subsp. lactis strains isolated from traditional fermented milk products in Turkey. International Journal of Food Science and Technology 35, 473–481.CrossRefGoogle Scholar
- 3.Allison, G. E., Klaenhammer, T. R., (1998) Phage resistance mechanisms in lactic acid bacteria. Int. Dairy J. 8, 207–226.CrossRefGoogle Scholar
- 4.Brüssow, H., Desire, F. (2001) Comparative phage genomics and the evolution of Siphoviridae: Insights from dairy phages. Mol. Microbiol. 39, 213–222.CrossRefGoogle Scholar
- 5.Chopin, M. C., Chopin, A., Bidnenko, E. (2005) Phage abortive infection in lactococci: Variation on a theme. Current Opinion in Microbiology 8, 473–479.CrossRefGoogle Scholar
- 6.Daly, C., Fitzgerald, G. F., Davis, R. (1996) Biotechnology of lactic acid bacteria with special reference to bacteriophage resistance. Antonie van Leeuwenhoek 70, 99–110.CrossRefGoogle Scholar
- 7.Deng, Y.-M., Harvey, M. L., Lui, C. Q., Dunn, N. W., (1997) A novel plasmid encoded phage abortive infection system from Lactococcus lactis biovar. diacetylactis. FEMS Microbiology Letters 146, 149–154.CrossRefGoogle Scholar
- 8.Desiere, F., Lucchini, S., Canchaya, C., Ventura, M., Brüssow, H. (2002) Comparative genomics of phages and prophages in lactic acid bacteria. Antonie van Leeuwenhoek 82, 73–91.CrossRefGoogle Scholar
- 9.Dinsmore, P. K., Klaenhammer, T. R., (1995) Bacteriophage resistance in Lactococcus. Mol. Biotechnology 4, 297–313.CrossRefGoogle Scholar
- 10.Garvey, P., Hill, C., Fitzgerald, G. F., (1996) The lactococcal plasmid pNP40 encodes a third bacteriophage resistance mechanisms, one which affects phage DNA penetration. Appl. Environ. Microbiol. 62, 676–679.PubMedPubMedCentralGoogle Scholar
- 11.Higgins, D. L., Sanozky, R. B., Klaenhammer, T. R., (1988) Restriction and modification activities from Streptococcus lactis ME2 are encoded by a self-transmissible plasmid, pTN 20, that forms cointegrates during mobilization of lactose-fermenting ability. J. Bacteriol. 170, 3435–3442.CrossRefGoogle Scholar
- 12.Hill, C. (1993) Bacteriophage and bacteriophage resistance in lactic acid bacteria. FEMS Microbiology Reviews 12, 87–108.CrossRefGoogle Scholar
- 13.Hill, C., Massey, I. J., Klaenhammer, T. R., (1991) Rapid method to characterize lactococcal bacteriophage genomes. Appl. Environ. Microbiol. 57, 286–288.Google Scholar
- 14.Jarvis, A. W., Fitzgerald, G. F., Mata, M., Mercenier, A., Neve, H., Powell, I. B., Rondo, C., Saxelin, M., Teuber, M. (1991) Species and types phages of lactococcal bacteriophages. Intervirology 32, 2–9.CrossRefGoogle Scholar
- 15.Josephsen, J., Madsen, A. (2001) The LlaG1 restriction and modification system of Lactococcus lactis W10 consists of only one single polypeptide. FEMS Microbiology Letters 200, 91–96.CrossRefGoogle Scholar
- 16.Lauridsen, B. S., Janzen, T., Schnabl, J., Johansen, E. (2003) Identification of the host determinants of two prolate-headed phages infecting Lactococcus lactis. Virology 309, 10–17.CrossRefGoogle Scholar
- 17.Lucey, M., Daly, C., Fitzgerald, G. F., (1992) Cell surface characteristics of Lactococcus lactis harboring pCI 528 a 46 kb plasmid encoding inhibition of phage adsorption. J. Gen. Microbiol. 138, 2137–2143.CrossRefGoogle Scholar
- 18.Madera, C., Garcia, P., Janzen, T., Rodriguez, A., Suárez, E. J. (2003) Characterization of the technologically proficient wild Lactococcus lactis strains resistant to phage infection. International Journal of Food Microbiology 86, 213–222.CrossRefGoogle Scholar
- 19.McGrath, S., Seegers, J. F., M. L., Fitzgerald, G. F., Van Sinderen, D. (1999) Molecular characterization of a phage encoded resistance system in Lactococcus lactis. Appl. Environ. Microbiol. 65, 1891–1999.PubMedPubMedCentralGoogle Scholar
- 20.McGrath, S., Fitzgerald, G. F., Van Sinderen, D. (2001) Improvement and optimization of two engineered phage resistance mechanisms in Lactococcus lactis. Appl. Environ. Microbiol. 67, 608–616.CrossRefGoogle Scholar
- 21.McGrath, S., Fitzgerald, G. F., Van Sinderen, D. (2004) The impact of bacteriophage genomics. Current Opinion in Biotechnology 15, 94–99.CrossRefGoogle Scholar
- 22.McKay, L. L., Baldwin, K. A., Zottola, E. A., (1972) Loss of lactose metabolism in lactic Streptococci. Appl. Microbiol. 23, 1090–1096.PubMedPubMedCentralGoogle Scholar
- 23.Moineau, S., Levesque, C. (2005) Control of bacteriophages in industrial fermentations. In: Kutter, E., Sulakvelidze, A. (eds) Bacteriophages: Biology and Applications. CRC Press, Chap. 10, pp. 285–296.Google Scholar
- 24.O’Sullivan, D., Ross, R. P., Twomey, D. P., Hill, C. (2001) Naturally occurring lactococcal plasmid pAH90 links bacteriophage resistance and mobility function to a food grade selectable marker. Appl. Environ. Microbiol. 67, 929–938.CrossRefGoogle Scholar
- 25.Sturnio, J. M., Klaenhammer, T. R., (2004) Bacteriophage defense systems and strategies for lactic acid bacteria. Adv. Appl. Microbiol. 56, 331–378.CrossRefGoogle Scholar
- 26.Terzaghi, B. E., Sandine, W. E., (1975) Improved medium for lactic streptococci and their bacteriophage. Appl. Microbiol. 29, 807–813.PubMedPubMedCentralGoogle Scholar
- 27.Twomey, D. P., Gabillet, N., Daly, C., Fitzgerald, G. F., (1997) Molecular characterization of the restriction endonuclease gene (ScrFI) associated with the ScrFI restriction/modification system from Lactococcus lactis subsp. cremoris UC503. Microbiology 143, 2277–2286.CrossRefGoogle Scholar
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