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Acta Biologica Hungarica

, Volume 57, Issue 1, pp 133–136 | Cite as

Formaldehyde and Some Fully N-Methylated Substances in Boar Seminal Fluids

Short Communication
  • M. SzilágyiEmail author
  • Zs. I. Németh
  • L. Albert
  • P. Sarlós
  • E. Tyihák
Open Access
Article

Abstract

On the basis of recent observations it is supposed that seminal fluids may contain — mainly in hydroxymethyl groups — formaldehyde (HCHO) and quaternary ammonium compounds as potential HCHO generators, therefore, preliminary investigations were carried out for the identification of these compounds in pig seminal fluids using OPLC, HPLC and MALDI MS techniques. The fresh pig seminal fluid was frozen in liquid nitrogen, powdered and aliquots (0.25 g) were treated with 0.7 ml ethanolic dimedone solution. The suspension was centrifuged and the clear supernatant was used for analysis by OPLC or after dilution with HPLC or MALDI MS technique. After OPLC separation of formaldemethone the fully N-methylated compounds which are stayed on the start point were separated by OPLC using an other eluent system. It has been established that the HCHO is really a normal component of the pig seminal fluid, as well. It can be isolated and identified in dimedone adduct form. The measurable amount of HCHO depended on the concentration applied of dimedone. According to OPLC and MALDI MS investigations L-carnitine is the main quaternary ammonium compound in pig seminal fluid which can generate a protection of the sperm cells against environmental and other influences. Considerable differences have been found among individuals concerning concentrations of quaternary ammonium compounds in the seminal fluid of pigs.

Keywords

Formaldehyde carnitine trigonelline seminal fluid pig 

Notes

Acknowledgements

The skillful assistance of Mrs Edit Dobos-Harsányi is gratefully acknowledged.

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Copyright information

© Akadémiai Kiadó, Budapest 2006

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • M. Szilágyi
    • 1
    Email author
  • Zs. I. Németh
    • 2
  • L. Albert
    • 2
  • P. Sarlós
    • 1
  • E. Tyihák
    • 3
  1. 1.Research Institute for Animal Breeding and NutritionHerceghalomHungary
  2. 2.Institute of ChemistryUniversity of West-HungarySopronHungary
  3. 3.Plant Protection InstituteHungarian Academy of SciencesBudapestHungary

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