Advertisement

Acta Biologica Hungarica

, Volume 56, Issue 3–4, pp 345–357 | Cite as

Genotypic Analysis of Variability in Zygomycetes

A Mini-Review
  • M. TakóEmail author
  • Á. Csernetics
Article

Abstract

Different types of molecular markers are available for use in evolutionary and population studies of microscopic fungi. These approaches have proved their merits and have been successfully applied to a wide range of fungal species belonging in the Ascomycetes and Basidiomycetes. Species in the class Zygomycetes have been rather neglected from this aspect. This review discusses the information available from investigations of the genotypic variability in this group of fungi.

Keywords

Genetic variability molecular markers Zygomycetes 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Abbas, J. D., Hetrick, B. A. D., Jurgenson, J. E. (1996) Isolate specific detection of mycorrhizal fungi using genome specific primer pairs. Mycologia 46, 939–946.CrossRefGoogle Scholar
  2. 2.
    Ács, K., Kasza, Zs., Lukács, Gy., Schwab, H., Vágvölgyi, Cs. (2002) Cloning and sequence analysis of Mucor circinellodes glyceraldehyde-3-phosphate dehydrogenase gene. Acta Microbiol. Immunol. Hung. 46, 305–312.CrossRefGoogle Scholar
  3. 3.
    Beadle, J., Wright, M., McNeely, L., Bennett, J. W. (2003) Electrophoretic karyotype analysis in fungi. Adv. Appl. Microbiol. 46, 243–270.CrossRefGoogle Scholar
  4. 4.
    Bidochka, M. J., Walsh, S. R. A., Ramos, M. E., Stleger, R. J., Silver, J. C., Roberts, D. W. (1995) Pathotypes in the Entomophaga grylli species complex of grasshopper pathogens differentiated with random amplification of polymorphic DNA and cloned DNA probes. Appl. Environ. Microbiol. 46, 556–560.Google Scholar
  5. 5.
    Buck, K. W. (1986) Fungal virology: an overview. In: Buck, K. W. (ed.) Fungal Virology. CRC Press: Boca Raton, Florida, USA, pp. 1–84.Google Scholar
  6. 6.
    Buck, K. W. (1998) Molecular variability of viruses of fungi. In: Bridge, P. D., Couteaudier, Y., Clarkson, J. M. (eds) Molecular Variability of Fungal Pathogens. CAB International, Wallingford, pp. 53–72.Google Scholar
  7. 7.
    Burmester, A., Wöstemeyer, J. (1994) Variability in genome organization of the zygomycete Parasitella parasitica. Curr. Genet. 46, 456–460.CrossRefGoogle Scholar
  8. 8.
    Carter, A. T., Mackenzie, D. A., Pearson, B. M., Archer, D. B. (1999) PCR fingerprinting with consensus tRNA primer enables strain identification of Mortierella alpina. Mycol. Res. 46, 794–799.Google Scholar
  9. 9.
    Chakrabarti, A., Ghosh, A., Prasad, G. S., David, J. K., Gupta, S., Das, A., Sakhuja, V., Panda, N. K., Singh, S. K., Das, S., Chakrabarti, T. (2003) Apophysomyces elegans: an emerging zygomycete in India. J. Clin. Microbiol. 46, 783–788.CrossRefGoogle Scholar
  10. 10.
    Diaz-Minguez, J. M., Lopez-Matas, M. A., Eslava, A. P. (1999) Complementary mating types of Mucor circinelloides show electrophoretic heterogeneity. Curr. Genet. 46, 383–389.Google Scholar
  11. 11.
    Federici, B. A., Humber, R. A. (1977) A possible baculovirus in the insect parasitic fungus, Strongwellsea magna. J. Gen. Virol. 46, 387–391.CrossRefGoogle Scholar
  12. 12.
    Ghabrial, S. A. (1994) New developments in fungal virology. Adv. Virus Res. 46, 303–388.CrossRefGoogle Scholar
  13. 13.
    Guarro, J., Gene, J., Stichigel, A. M. (1999) Developments in fungal taxonomy. Clin. Microbiol. Rev. 46, 454–500.CrossRefGoogle Scholar
  14. 14.
    Hajek, A. E. (1997) Ecology of terrestrial fungal entomopathogens. In: Jones (ed.) Advances in Microbial Ecology, Vol. 15. Plenum Press, New York, pp. 193–249.CrossRefGoogle Scholar
  15. 15.
    Hajek, A. E., Jensen, A. B., Thomsen, L., Hodge, K. T., Eilenberg, J. E. (2003) PCR-RFLP is used to investigate relations among species in the entomopathogenic genera Eryniopsis and Entomophaga. Mycologia 46, 262–268.CrossRefGoogle Scholar
  16. 16.
    Havens, P. L. (1976) Comparative zone electrophoresis and mating experiments in the taxonomy of Mucor hiemalis. Mycotaxon 46, 218–232.Google Scholar
  17. 17.
    Helgason, T., Daniell, T. J., Husband, R., Fitter, A. H., Young, J. P. W. (1998) Ploughing up the woodwide web? Nature 394, 431.CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Hodge, K. T., Sawyer, A. J., Humber, R. A. (1995) RAPD-PCR for identification of Zoophtora radicans isolates in biological control of the potato leafhopper. J. Inverteb. Pathol. 46, 1–9.CrossRefGoogle Scholar
  19. 19.
    Jensen, A. B., Gargas, A., Eilenberg, J., Rosendahl, S. (1998) Relationships of the insect-pathogenic order Entomophthorales (Zygomycota, Fungi) based on phylogenetic analysis of nuclear small subunit ribosomal DNA (SSU rDNA). Fung. Genet. Biol. 46, 325–334.CrossRefGoogle Scholar
  20. 20.
    Kayser, T., Wöstemeyer, J. (1991) Electrophoretic karyotype of the zygomycete Absidia glauca: evidence for differences between mating types. Curr. Genet. 46, 279–284.CrossRefGoogle Scholar
  21. 21.
    Kohn, L. M. (1992) Developing new characters for fungal systematics: an experimental approach for determining the rank of resolution. Mycologia 46, 139–153.CrossRefGoogle Scholar
  22. 22.
    Lemmer, K., Losert, H., Rickerts, V., Just-Nübling, G., Sander, A., Kekrmann, M.-L., Tintelnot, K. (2002) Identification of Cunninghamella spec. by molecular methods. Mycoses 45 (Suppl. 1), 31–36.CrossRefPubMedPubMedCentralGoogle Scholar
  23. 23.
    Lusta, K. A., Kochkina, G. A., Sul, I. W., Chung, I. K., Park, H. S., Shin, D. (2003) An integrated approach to taxonomical identification of the novel filamentous fungus strain producing extracellular lipases: morphological, physiological and DNA fingerprinting techniques. Fung. Diversity 46, 135–149.Google Scholar
  24. 24.
    Marier, J.-F., Vago, C., Devauchelle, G., Duthoit, J.-L. (1971) Infection virale chez les Trichomycetes. C. R. Acad. Sci., Paris, Ser. D 273, 1241.Google Scholar
  25. 25.
    Micales, J. A., Bonde, M. R., Peterson, G. L. (1992) Isozyme analysis in fungal taxonomy and molecular genetics. In: Arora, D. K., Elander, R. P., Murekji, K. G. (eds) Handbook of Applied Mycology. Marcel Dekker, Inc., 57–79.Google Scholar
  26. 26.
    Murrin, F., Newcomb, W. (1987) Ultrastructure of virus-like particles in hyphae of Entomophaga aulicae (Zygomycetes). Mycologia 46, 644–649.CrossRefGoogle Scholar
  27. 27.
    Nagy, Á., Palágyi, Zs., Vastag, M., Ferenczy, L., Vágvölgyi, Cs. (2000) Electrophoretic karyotypes of some related Mucor species. Antonie van Leeuwenhoek 46, 33–37.CrossRefGoogle Scholar
  28. 28.
    Nagy, Á., Pesti, M., Galgóczy, L., Vágvölgyi, Cs. (2004) Electrophoretic karyotype of two Micromucor species. J. Basic Microbiol. 46, 36–41.CrossRefGoogle Scholar
  29. 29.
    Nagy, Á., Vágvölgyi, Cs., Balla, É., Ferenczy, L. (1994) Electrophoretic karyotype of Mucor circinelloides. Curr. Genet. 46, 45–48.CrossRefGoogle Scholar
  30. 30.
    Nelson, R. T., TeStrake, D., Cochrane, B. J. (1998) The distribution of Basidiobolus and Conidiobolus in soil and litter at sites near Tampa, Florida. Mycologia 46, 761–766.CrossRefGoogle Scholar
  31. 31.
    Nyilasi, I., Papp, T., Heinrich, H., Lukács, Gy., Vágvölgyi, Cs. (2002) Identification of mating-typespecific markers in Gilbertella persicaria. Acta Microbiol. Immunol. Hung. 46, 404–405.Google Scholar
  32. 32.
    O’Donnell, K., Lutzoni, F. M., Ward, T. J., Benny, G. L. (2001) Evolutionary relationships among mucoralean fungi (Zygomycota): Evidence for family polyphyly on a large scale. Mycologia 46, 286–296.CrossRefGoogle Scholar
  33. 33.
    Papp, T., Ács, K., Kasza, Zs., Galgóczy, L., Nagy, E., Vágvölgyi, Cs. (2003) Phylogenetic relationship of the genus Gilbertella and related genera within the Mucorales based on rDNA sequences. Acta Biol. Immunol. Hung. 46, 393–402.CrossRefGoogle Scholar
  34. 34.
    Papp, T., Lukács, Gy., Vastag, M., Vágvölgyi, Cs., Nagy, E. (2004) Identification of Rhizomucor species by means of biochemical and molecular methods. Clin. Microbiol. Infect. 10, Suppl. 3. 508.Google Scholar
  35. 35.
    Papp, T., Nagy, Á., Palágyi, Zs., Vastag, M., Vágvölgyi, Cs. (1999) Genetic studies on sexual processes of Gilbertella persicaria. Acta Microbiol. Immunol. Hung. 46, 327–328.Google Scholar
  36. 36.
    Papp, T., Nyilasi, I., Fekete, Cs., Ferenczy, L., Vágvölgyi, Cs. (2001) Presence of double-stranded RNA and virus-like particles in Rhizopus isolates. Can. J. Microbiol. 46, 443–447.CrossRefGoogle Scholar
  37. 37.
    Papp, T., Palágyi, Zs., Ferenczy, L., Vágvölgyi, Cs. (1999) The mitochondrial genome of Mucor piriformis. FEMS Microbiol. Lett. 46, 67–72.CrossRefGoogle Scholar
  38. 38.
    Papp, T., Vágvölgyi, Cs., Kerényi, Z., Nagy, Á., Michailides, T. J. (1997) DNA amplification polymorphisms of Mucor piriformis. Ant. Leeuwenhoek 46, 167–173.CrossRefGoogle Scholar
  39. 39.
    Papp, T., Vastag, M., Ferenczy, L., Vágvölgyi, Cs. (2000) Investigation of the genetic variability of Gilbertella persicaria via molecular markers. ECFG-5, Arcachon, Abstracts 314.Google Scholar
  40. 40.
    Papp, T., Vastag, M., Ferenczy, L., Vágvölgyi, Cs. (2000) Phylogenetic position of Gilbertella persicaria inferred from glyceraldehyde-3-phosphate dehydrogenase gene sequences. ECFG-5, Arcachon, Abstracts 315.Google Scholar
  41. 41.
    Papp, T., Vastag, M., Nagy, Á., Michailides, T. J., Vágvölgyi, Cs. (2001) Genetic variability of the postharvest pathogen Gilbertella persicaria: identification of randomly amplified polymorphic DNA (RAPD) markers correlating with (+) and (-) mating types. Ant. Leeuwenhoek 46, 301–309.CrossRefGoogle Scholar
  42. 42.
    Papp, T., Vastag, M., Vágvölgyi, Cs. (1999) Genetic variability of the postharvest pathogen Gilbertella persicaria. Acta Microbiol. Immunol. Hung. 46, 343–344.Google Scholar
  43. 43.
    Paquin, B., Laforest, M.-J., Forget, L., Roewer, I., Wang, Z., Longcore, J., Lang, B. F. (1997) The fungal mitochondrial genomes and their expression. Curr. Genet. 46, 380–395.CrossRefGoogle Scholar
  44. 44.
    Quiles-Rosillo, M. D., Ruiz-Vazquez, R. M., Torres-Martinez, S., Garre, V. (2003) Cloning, characterization and heterologous expression of the Blakeslea trispora gene encoding orotidine-5-monophosphate decarboxylase. FEMS Microbiol. Letters 46, 229–236.CrossRefGoogle Scholar
  45. 45.
    Radford, A. (1993) A fungal phylogeny based upon orotidine 5’-monophosphate decarboxylase. J. Mol. Evol. 46, 389–395.Google Scholar
  46. 46.
    Redecker, D. (2000) Specific PCR primers to identify arbuscular mycorrhizal fungi with colonized roots. Mycorrhiza 46, 73–80.CrossRefGoogle Scholar
  47. 47.
    Redecker, D., Morton, J. B., Bruns, T. D. (2000) Ancestral lineages of arbuscural mycorrhyzal fungi (Glomales). Mol. Phylogen. Evol. 46, 276–284.CrossRefGoogle Scholar
  48. 48.
    Redecker, D., Thierfelder, H., Walker, C., Werner, D. (1997) Restriction analysis of PCR-amplified internal transcribed spacers of ribosomal DNA as a tool for species identification in different genera of the order Glomales. Appl. Environ. Microbiol. 46, 1756–1761.Google Scholar
  49. 49.
    Sanders, I. R., Alt, M., Groppe, K., Boller, T., Wiemken, A. (1995) Identification of ribosomal DNA polymorphisms among and within spores of the Glomales: Application to studies on the genetic diversity of arbuscular mycorrhizal fungal communities. New Phytol. 46, 419–427.CrossRefGoogle Scholar
  50. 50.
    Schipper, M. A. A. (1973) A study on variability in Mucor hiemalis and related species. Stud. Mycol. 46, 1–40.Google Scholar
  51. 51.
    Schipper, M. A. A. (1978) On the genera Rhizomucor and Parasitella. Stud. Mycol. 46, 53–71.Google Scholar
  52. 52.
    Schipper, M. A. A. (1984) A revision of the genus Rhizopus. I. The Rhizopus stolonifer-group and Rhizopus oryzae. Stud. Mycol. 46, 1–19.Google Scholar
  53. 53.
    Schramke, M. L., Orlowski, M. (1993) Mitochondrial genome of the dimorphic zygomycete Mucor racemosus. Curr. Genet. 46, 337–343.CrossRefGoogle Scholar
  54. 54.
    Seif, E., Leight, J., Liu, Y., Roewer, I., Forget, L., Lanf, B. F. (2005) Comparative mitochondrial genomics in zygomycetes: bacteria-like RNase P RNAs, mobile elements and a close source of the group I intron invasion in angiosperms. Nucl. Acid. Res. 46, 734–744.CrossRefGoogle Scholar
  55. 55.
    Simon, L., Lalonde, M., Bruns, T. D. (1992) Specific amplification of 18S fungal ribosomal genes from vesicular-arbuscular endomycorrhizal fungi colonizing roots. Appl. Environ. Microbiol. 46, 291–295.Google Scholar
  56. 56.
    Stout, D. L., Shaw, C. R. (1974) Genetic distance among certain species of Mucor. Mycologia 46, 969–977.CrossRefGoogle Scholar
  57. 57.
    Taylor, J. W., Geiser, D. M., Burt, A., Koufopanou, V. (1999) The evolutionary biology and population genetics underlying fungal strain typing. Clin. Microbiol. Rev. 46, 126–146.CrossRefGoogle Scholar
  58. 58.
    Thomsen, L., Jensen, A. B. (2002) The distribution of Basidiobolus and Conidiobolus in soil and litter at sites near Tampa, Florida. Mycologia 46, 794–802.CrossRefGoogle Scholar
  59. 59.
    Tudzynski, P., Weltring, K-M. (1993) Molecular genetics of phytopathogenic fungi. Progress in Botany 46, 358–372.Google Scholar
  60. 60.
    Tymon, A. M., Shah, P. A., Pell, J. K. (2004) PCR-based molecular discrimination of Pandora neoaphidis isolates from related entomopathogenic fungi and development of species-specific diagnostic primers. Mycol. Res. 46, 419–433.CrossRefGoogle Scholar
  61. 61.
    Vágvölgyi, Cs., Heinrich, H., Ács, K., Papp, T. (2004) Genetic variability in the species Rhizopus stolonifer, assessed by random amplified polymorphic DNA analysis. Antonie van Leeuwenhoek 46, 181–188.CrossRefGoogle Scholar
  62. 62.
    Vágvölgyi, Cs., Kasza, Zs., Nyilasi, I., Ács, K., Papp, T. (2001) Variability of isozyme and RAPD markers among isolates of Mucor genevensis. Acta Biol. Immunol. Hung. 46, 365–373.Google Scholar
  63. 63.
    Vágvölgyi, Cs., Klaudia, M., Papp, T., Vastag, M., Ferenczy, L., Hornok, L., Fekete, Cs. (1998) Detection of double-stranded RNA molecules and virus-like particles in different Mucor species. Ant. van Leeuwenhoek 46, 207–210.CrossRefGoogle Scholar
  64. 64.
    Vágvölgyi, Cs., Lukács, Gy., Nyilasi, I., Papp, T. (2004) Development of a lovastatin resistancebased transformation system for Rhizomucor miehei. Clin. Microbiol. Inf. 10 (S3), 507.Google Scholar
  65. 65.
    Vágvölgyi, Cs., Manczinger, L. (1990) Separation of chromosomes from Mucor circinelloides. In: Reisinger, A., Bresinsky, A. (eds) IMC4. Regensburg, Abstracts 344.Google Scholar
  66. 66.
    Vágvölgyi, Cs., Papp, T., Palágyi, Zs., Michailides, T. J. (1996) Isozyme variation among isolates of Mucor piriformis. Mycologia 46, 602–607.CrossRefGoogle Scholar
  67. 67.
    Vágvölgyi, Cs., Varga, J., Ferenczy, L. (1993) Detection of double-stranded RNA in Mucor ramannianus. Fungal Genet. Newsl. 40, 79.Google Scholar
  68. 68.
    Vágvölgyi, Cs., Vastag, M., Ács, K., Papp, T. (1999) Rhizomucor tauricus: a questionable species of the genus. Mycol. Res. 46, 612–618.Google Scholar
  69. 69.
    van Tuinen, D., Jacquot, E., Zhao, B., Gollotte, A., Gianinazzi-Pearson, V. (1998) Characterization of root colonization profiles by a microcosm community of arbuscular mycorrhizal fungi using 25rDNA-targeted nested PCR. Mol. Ecol. 46, 879–887.CrossRefGoogle Scholar
  70. 70.
    Varga, J., Tóth, B., Vágvölgyi, Cs. (2003) Recent advances in mycovirus research. Acta Microbiol. Immunol. Hung. 46, 77–94.CrossRefGoogle Scholar
  71. 71.
    Varga, J., Vágvölgyi, Cs. (1991) Isoenzyme analysis of some mesophyl Mucor strains. Acta Microbiol. Immunol. Hung. 38, 224.Google Scholar
  72. 72.
    Vastag, M., Kasza, Zs., Ács, K., Papp, T., Schwab, H., Vágvölgyi, Cs. (2004) Cloning and sequences analysis of the glyceraldehyde-3-phosphate dehydrogenase gene from the zygomycetes fungus Rhizomucor miehei. Ant. Leeuwenhoek 86, 111–119.CrossRefGoogle Scholar
  73. 73.
    Vastag, M., Papp, T., Kasza, Zs., Vágvölgyi, Cs. (1998) Differentiation of Rhizomucor species by means of carbon source utilization and isoenzyme analysis. J. Clin. Microbiol. 46, 2153–2156.Google Scholar
  74. 74.
    Vastag, M., Papp, T., Kasza, Zs., Vágvölgyi, Cs. (2000) Intraspecific variation in two species of Rhizomucor assessed by random amplified polymorphic DNA analysis. J. Basic Microbiol. 46, 269–277.CrossRefGoogle Scholar
  75. 75.
    Voigt, K., Cigelnik, E., O’Donnell, K. (1999) Phylogeny and PCR identification of clinically important zygomycetes based on nuclear ribosomal-DNA sequence data. J. Clin. Microbiol. 46, 3957–3964.Google Scholar
  76. 76.
    Voigt, K., Wöstemeyer, J. (2001) Phylogeny and origin of 82 zygomycetes from all 54 genera of the Mucorales and Mortierellales based on combined analysis of actin and translation elongation factor EF-1α genes. Gene 46, 113–120.CrossRefGoogle Scholar
  77. 77.
    Zolan, M. E. (1995) Chromosome-length polymorphism in fungi. Microbiol. Rev. 46, 686–698.Google Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2005

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Department of Microbiology, Faculty of SciencesUniversity of SzegedSzegedHungary

Personalised recommendations