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Acta Biologica Hungarica

, Volume 56, Issue 3–4, pp 177–183 | Cite as

L-Glutamate and Phorbol Ester Stimulate The Release of Secretory Amyloid Precursor Protein from Rat Cortical Synaptosomes

  • L. KirazovEmail author
  • E. Kirazov
  • R. Schliebs
Article

Abstract

Treatment of rat cortical synaptosomes with micromolar concentrations of L-glutamate stimulated the release of the secreted form of amyloid precursor protein in a concentration-dependent, however biphasic manner as assayed by semiquantitative Western blot analysis. The secreted amyloid precursor protein released from synaptosomes into the incubation medium was highest in the presence of 500 μM L-glutamate (about 64% over the level assayed in the incubation medium in the absence of any drug). In contrast, direct stimulation of protein kinase C by phorbol-12-myristate-13-acetate resulted in a concentration- independent increase in secretory amyloid precursor protein release by about 100% already detectable at a concentration of 0.1 μM but with no significant change at higher concentrations up to 10 μM. The presented data show that there is a constitutive release of secretory amyloid precursor protein from synaptosomes and suggest that (i) processing of amyloid precursor protein at the synaptic level is controlled by L-glutamate presumably via activation of protein kinase C, and (ii) isolated cortical synaptosomes represent a useful experimental approach to selectively study amyloid precursor protein metabolism at the synaptic level.

Keywords

Secreted amyloid precursor protein synaptosomes glutamate phorbol ester protein kinase C 

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© Akadémiai Kiadó, Budapest 2005

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Department of Neuromorphology, Institute of Experimental Morphology and AnthropologyBulgarian Academy of SciencesSofiaBulgaria
  2. 2.Department of NeurochemistryPaul Flechsig Institute for Brain ResearchLeipzigGermany

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