Acta Biologica Hungarica

, Volume 56, Issue 1–2, pp 21–34 | Cite as

Antinociceptive Action of Aqueous Extract of The Leaves of Ixora Coccinea

  • W. D. RatnasooriyaEmail author
  • S. A. Deraniyagala
  • S. D. N. K. Bathige
  • C. L. Goonasekara
  • J. R. A. C. Jayakody


The aim of this study was to examine the antinociceptive potential of leaves of Ixora coccinea (family: Rubeaceae). One of four doses (500, 750, 1000 or 1500 mg/kg, n=8/dose) of aqueous leaf extract (ALE) or 1 ml of distilled water was orally administered to male rats and antinociceptive activity was ascertained using three models of nociception (tail flick, hot plate and formalin tests). The results showed that ALE possesses considerable antinociceptive activity (when evaluated in hot plate and formalin test but not in tail flick test). The antinociceptive activity of the ALE had a rapid onset (within 1 h) and a fairly long duration of action (up to 5 h) with a peak effect at 3 h. Further, the antinociceptive activity was dose-dependent and was not associated with harmful side-effects or toxicity even following subchronic administration. The antinociceptive action was mediated centrally at the supraspinal level mainly via dopaminergic mechanism. In addition, it is likely that antioxidant activity of the ALE could have played an auxiliary role in inducing antinociception. Dopaminergic and antioxidative activities of ALE could arise, respectively, from its quaternary base alkaloid and flavonoid constituents.


Ixora coccinea antinociception pain toxicology 


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  1. 1.
    Anonymous, British National Formulary 2000. The British Medical Association and the Royal Pharmaceutical Society of Great Britain, London.Google Scholar
  2. 2.
    Bowers, M. B., Van Woert, M., Davis, L. (1971) Sexual behavior during L-dopa treatment for Parkinsonism. Am. J. Psychiatry 127, 1691–1693.CrossRefGoogle Scholar
  3. 3.
    de Campos, R. O. P., Santos, A. R. S., Vaz, Z. R., Pinheiro, T. R., Pizzolatti, M. G., Filho, V. C., Monache, F. D., Yunes, R. A., Calixto, J. B. (1997) Antinociceptive properties of the hydroalcoholic extract and preliminary study of a xanthone isolated from Polygala cyparissias. Life Sci. 61, 1619–1630.CrossRefGoogle Scholar
  4. 4.
    Deraniyagala, S. A., Ratnasooriya, W. D., Goonesekera, C. L. (2003) Antinociceptive effect and tox-icological study of the aqueous bark extract of Barringtonia racemosa on rats. J. Ethnopharmacol. 2846, 1–6.Google Scholar
  5. 5.
    Dharmasiri, M. G., Ratnasooriya, W. D., Thabrew, M. I. (2003) Water extract of leaves and stems of pre-flowering but not flowering plants of Anisomeles indica possesses analgesic and antihyperalgesic activity in rats. Pharm. Biol. 41, 37–44.CrossRefGoogle Scholar
  6. 6.
    Dorman, H. S. D., Deans, S. G., Noble, R. C. (1995) Evaluation in vitro of plant essential oils as natural antioxidants. J. Essent. Oil Res. 7, 645–651.CrossRefGoogle Scholar
  7. 7.
    Farnsworth, N. R. (1998) Phytochemical Screening. Chicago College of Pharmacy, University of Illinois, pp. 32–65.Google Scholar
  8. 8.
    Farsam, H., Amanlou, M., Dehpour, A. Z., Jahaniani, F. (2000) Anti-inflammatory and analgesic activity of Biebersteinia multifida DC. root extract. J. Ethnopharmaco. 71, 443–447.CrossRefGoogle Scholar
  9. 9.
    File, S. E., Wardill, A. (1975) Validity of head dipping as a measure of exploration in modified holeboard. Psychopharmacologia 44, 53–57.CrossRefGoogle Scholar
  10. 10.
    Ghai, C. L. (1993) A Textbook of Practical Physiology. Jaypee Brothers Medical Publishers Ltd., New Delhi, pp. 119–202.Google Scholar
  11. 11.
    Halliwell, B. (1994) Free radical antioxidants in human disease: Curiosity, cause or consequence. Lancet 344, 721–724.CrossRefGoogle Scholar
  12. 12.
    Hull, E. M., Du, J., Lorrain, D. S., Matuszewich, L. (1997) Testosteron, preoptic dopamine, and copulation in male rats. Brain Res. Bull. 44, 327–333.CrossRefGoogle Scholar
  13. 13.
    Jayaweera, D. M. A. (1982) Medicinal Plants Used in Ceylon, part I V. National Science Council of Sri Lanka, Colombo, Sri Lanka.Google Scholar
  14. 14.
    Kaseda, S., Nomoto, M., Iwata, S. (1999) Effect of selegiline on dopamine concentration in the stria-tum of a primate. Brain Res. 815, 44–50.CrossRefGoogle Scholar
  15. 15.
    Latha, P. G., Nayar, M. N. S., Sing, O. V., George, K. R., Panikkar, K. R., Pushpangadan, P. (2001) Isolation of antigenotoxic ursolic acid from Ixora coccinea flowers. Actual Biol. 23, 21–24.Google Scholar
  16. 16.
    Latha, P. G., Panikkar, K. R. (1998) Cytotoxic and antitumor principles from Ixora coccinea flowers. Cancer Lett. 130, 197–202.CrossRefGoogle Scholar
  17. 17.
    Latha, P. G., Panikkar, K. R. (2001) Chemoprotective effect of Ixora coccinea L. flowers on cisplatin induced toxicity in mice. Phytotherapy Res. 15, 364–366.CrossRefGoogle Scholar
  18. 18.
    Latha, P. G., Panikkar, K. R. (1999) Modulatory effects of Ixora coccinea flower on cyclophos-phamide-induced toxicity in mice. Phytotherapy Res. 13, 517–520.CrossRefGoogle Scholar
  19. 19.
    Luedtke, R. R., Freeman, R. A., Volk, M., Arfan, M., Reinecke, M. G. (2003) Pharmacological survey of medicinal plants for activity at dopamine receptor subtypes. II. Screen for binding activity at the D1 and D2 dopamine receptor subtypes. Pharmaceutical Biol. 41, 45–58.CrossRefGoogle Scholar
  20. 20.
    Miguel, O. G., Calixto, J. B., Santos, A. R. S., Messana, I., Ferrari, F., Filho, V. C., Pizzolatti, M. G., Yunes, R. A. (1996) Chemical and preliminary analgesic evaluation of Geraniin and Furosin isolated from Phyllanthus sellowianus. Planta Medica 62, 97–192.CrossRefGoogle Scholar
  21. 21.
    Rang, H. P., Dale, M. M., Ritter, J. M. (1995) Pharmacology. Churchill, Livingston, London.Google Scholar
  22. 22.
    Ratnasooriya, W. D., Dharmasiri, M. G. (1999) Water extracts of Psychotria sarmentosa has analgesic and antihyperalgesic activity in rats. Med. Sci. Res. 27, 715–718.Google Scholar
  23. 23.
    Ratnasooriya, W. D., Deraniyagala, S. A., Bathige, S. D. N. K., Galhena, G., Liyanage, S. S. P., Jayakody, J. R. A. C. (2004) Anti-inflammatory activity of the aqueous leaf extract of Ixora coccinea in rats. J. Pharm. Biol. (in press).Google Scholar
  24. 24.
    Ratnasooriya, W. D., Galhena, G., Liyanage, S. S. P., Jayakody, J. R. A. C., Ediriwcera, E. R. H. S. S. (2003) Analgesic and antihyperalgesic activities of decoction of Sri Lankan Scoparia dulcis in rats. J. Tropical Med. Plants (in press).Google Scholar
  25. 25.
    Reena, Z., Sudhakaran, N., Velayudha, C. R., Panicker, P. (1994) Anti-inflammatory and anti-mitot-ic activities of lupeol isolated from the leaves of Ixora coccinea Linn. Indian J. Pharm. Sci. 56, 129–132.Google Scholar
  26. 26.
    Richardson, J. D., Aanonsen, L., Hargreaves, K. M. (1998) Antihyperalgesic effects of spinal connabinoids. European J. Pharmacol. 345, 145–153.CrossRefGoogle Scholar
  27. 27.
    Sasidharan, V. K. (1997) Search for antibacterial and antifungal activity of some plants of Kerala. Acta Pharm. 47, 47–51.Google Scholar
  28. 28.
    Tjolsen, A., Berge, O. G., Hunskaar, S., Rosland, J. H., Hole, K. (1992) The formalin test: an evaluation of the method. Pain 51, 5–17.CrossRefGoogle Scholar
  29. 29.
    Wong, C. H., Day, P., Yarmush, J., Wu, W., Zbuzek, U. K. (1994) Nifedipine-induced analgesic after epidural injections in rats. Anesthesia Analgesia 79, 303–306.CrossRefGoogle Scholar

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© Akadémiai Kiadó, Budapest 2005

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • W. D. Ratnasooriya
    • 1
    Email author
  • S. A. Deraniyagala
    • 2
  • S. D. N. K. Bathige
    • 2
  • C. L. Goonasekara
    • 2
  • J. R. A. C. Jayakody
    • 1
  1. 1.Department of ZoologyUniversity of ColomboColombo-03Sri Lanka
  2. 2.Department of ChemistryUniversity of ColomboColombo-03Sri Lanka

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