Cryopreservation of European Eel (Anguilla anguilla) Sperm Using Different Extenders and Cryoprotectants
Experiments were carried out on the sperm cryopreservation of artificially induced eels. The effects of several extenders and two cryoprotectants on the motility of spermatozoa were investigated. The highest post-thaw motility was observed with the combination of Tanaka’s extender and DMSO as cryoprotectant. Further dilution after thawing resulted in complete loss of motility in samples frozen in presence of DMSO while sperm frozen with methanol as cryoprotectant retained its motility after further dilution.
KeywordsEel sperm cryopreservation methanol DMSO
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