Separate Distribution of Deutocerebral Projection Neurons in the Mushroom Bodies of the Cricket Brain

Abstract

Deutocerebral projection neurones in the brain of the cricket (Gryllus bimaculatus) have been investigated by experimental dextran staining, viewed by light and electron microscopy. These neurones of two separate somata clusters innervate two separate primary glomerular neuropils of the deutocerebral segment, either the antennal lobe receiving only antennal nerve sensory input, or the glomerular lobe, receiving input from sensory neurones of lower segmental origin, including chemosensory fibres from mouth parts. Projection neurones of the antennal lobe only invade the anterior calyx of the mushroom body neuropil via the inner antenno glomerular tract, while glomerular relay neurones of the glomerular lobe innervate only the posterior calyx via the tritocerebral tract. All types of projection neurones give rise to presynaptic boutons, forming the central core of microglomeruli with patterned distribution. These projection neurons are cholinergic. The results are discussed in view of maintained segregated modal information, first processed in the separated primary deutocerebral neuropiles and further on in the second order input neuropils of the mushroom bodies. The large posterior calyces are proposed as a compartment for gustatory information.

References

  1. 1.

    Anton, S., Homberg, U. (1999) Antennal lobe structure. In: Hansson, B. (ed.) Insect olfaction. Springer Verlag, Berlin, pp. 97–12

    Chapter  Google Scholar 

  2. 2.

    Blaney, W. M. (1974) Electrophysiological responses of the terminal sensilla on the maxillary palp of Locusta migratoria (L.) to some electrolytes and non electrolytes. J. Exp. Biol. 60, 275–293.

    CAS  PubMed  Google Scholar 

  3. 3.

    Ernst, K.-D., Boeckh, J., Boeckh, V. (1977) A neuroanatomical study on the organization of the central antennal pathways in insects. Cell Tiss. Res. 176, 285–308.

    CAS  Google Scholar 

  4. 4.

    Fiala, A., Spall, T., Diegelmann, S., Eisermann, B., Sachse, S., Devaud, J.-M., Buchner, E., Galizia, C. G. (2002) Genetically expressed cameleon in Drosophila melanogaster is used to visualize olfactory information in projection neurons. Curr. Biol. 12, 1877–1884.

    CAS  Article  Google Scholar 

  5. 5.

    Galizia, C. G., Menzel, R. (2001) The role of glomeruli in the neural representation of odours: results from optical recording studies. J. Ins. Physiol. 47, 115–129.

    Google Scholar 

  6. 6.

    Gronenberg, W. (1999) Modality-specific segregation of input to ant mushroom bodies. Brain Behav. Evol. 54, 85–95.

    CAS  Article  Google Scholar 

  7. 7.

    Ignell, R., Anton, S., Hansson, B. S. (2000) The maxillary palp sensory pathways of Orthoptera. Arthropod Structure & Development 29, 295–305.

    CAS  Article  Google Scholar 

  8. 8.

    Schildberger, K. (1984) Multimodal interneurons in the cricket brain: properties of extrinsic mushroom body cells. J. Comp. Physiol. (A) 154, 71–79.

    Article  Google Scholar 

  9. 9.

    Schürmann, F.-W. (1973) Über die Struktur der Pilzkörper des Insektenhirns. III. Die Anatomie der Nervenfasern in den Corpora pedunculata bei Acheta domesticus L. Z. Zellforsch. 145, 247–2

    Google Scholar 

  10. 10.

    Schürmann, F.-W. (1987) The architecture of the mushroom bodies and related neuropils in the insect brain. In: Gupta, A. P. (ed.) Arthropod Brain. John Wiley, New York, pp. 231–264.

    Google Scholar 

  11. 11.

    Staudacher, E., Schildberger, K. (1999/2000): A newly described neuropile in the deutocerebrum of the cricket: Antennal afferents and descending interneurons. Zoology 102, 212–226.

    Google Scholar 

  12. 12.

    Strausfeld, N. J. (2002) Organization of the honey bee mushroom body: Representation of the calyx within the vertical and gamma lobes. J. Comp. Neurol. 450, 4–33.

    Article  Google Scholar 

  13. 13.

    Strausfeld, N. J., Li, Y. S. (1999) Organization of olfactory and multimodal afferent neurons supplying the calyx and pedunculus of the cockroach mushroom bodies. J. Comp. Neurol. 409, 603–625.

    CAS  Article  Google Scholar 

  14. 14.

    Stocker, R. F., Schorderet, M. (1981) Cobalt filling of sensory projections from internal and external mouthparts in Drosophila. Cell Tiss. Res. 216, 513–523.

    CAS  Google Scholar 

  15. 15.

    Weiss, M. J. (1981) Structural patterns in the corpora pedunculata of orthoptera: a reduced silver analysis. J. Comp. Neurol. 203, 515–553.

    CAS  Article  Google Scholar 

  16. 16.

    Yasuyama, K., Meinertzhagen, I. A., Schürmann, F.-W. (2002) Synaptic organization of the mushroom body calyx in Drosophila melanogaster. J. Comp. Neurol. 445, 211–226.

    Article  Google Scholar 

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Correspondence to Ina Frambach.

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Presented at the 10th ISIN Symposium on Invertebrate Neurobiology, July 5–9, 2003, Tihany, Hungary.

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Frambach, I., Schürmann, FW. Separate Distribution of Deutocerebral Projection Neurons in the Mushroom Bodies of the Cricket Brain. BIOLOGIA FUTURA 55, 21–29 (2004). https://doi.org/10.1556/ABiol.55.2004.1-4.4

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Keywords

  • Mushroom bodies
  • deutocerebral projection neurones
  • dextran staining
  • synapses
  • Gryllus bimaculatus