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Acta Biologica Hungarica

, Volume 55, Issue 1–4, pp 287–291 | Cite as

Microglia Proliferation as a Response to Activation in the Freshwater Snail Planorbarius Corneus: a BrdU Incorporation Study

  • Elisa PeruzziEmail author
  • D. Sonetti
Article

Abstract

Invertebrate microglia constitute a class of cells resident in the ganglionic nervous system which are activated after tissue injury or by the presence of pathogens. The microglia activation response includes graduated morpho-functional and biochemical changes and cell proliferation. In this study we verified in the freshwater snail Planorbarius corneus that an activation caused by a traumatic event may induce microglia division. Cell proliferation was assessed immunocytochemically using BrdU incorporation technique and documented on both ganglionic sections and microglia cultured cells at different experimental conditions and times after activation. In addition, we studied the possibility of increasing microglia proliferation by adding to the cultured medium the Macrophage-Colony Stimulating Factor (M-CSF) that has been shown to stimulate specifically this process in vertebrates.

Keywords

Microglia BrdU proliferation Planorbarius corneus M-CSF 

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Copyright information

© Akadémiai Kiadó, Budapest 2004

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Dipartimento di Biologia AnimaleUniversit à di Modena e Reggio EmiliaModenaItaly

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