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Acta Biologica Hungarica

, Volume 55, Issue 1–4, pp 201–210 | Cite as

Serotonin, Nitric Oxide and Histamine Enhance the Excitability of Neuron MCC by Diverse Mechanisms

  • J. JackletEmail author
  • J. Grizzaffi
  • D. Tieman
Article

Abstract

Serotonin, nitric oxide (NO) and histamine are neuromodulators used in molluscan nervous systems. We have found that each of them depolarizes and increases the excitability of the serotonergic feeding neural circuit modulator neuron, MCC, of Aplysia, but each induces different changes in background ionic currents and uses a different second messenger. Stimulation of neuron C2 in the cerebral ganglion induces a vsEPSP in MCC using NO and histamine. When these neurons are isolated in culture they form synapses that mediate the vsEPSP. The ionic currents induced by these neuromodulators were investigated in isolated cultured MCCs. Histamine reduced a background outward current between -70 and -30 mV that was blocked by cobalt treatment, indicating that it is a calcium activated potassium current. Serotonin reduced a background outward current from -65 mV to -30 mV and enhanced a potassium inward current more negative than -70 mV that was blocked by cesium and barium. This response was mimicked by 8-Br-cAMP NO donors reduced a cobalt insensitive background outward current between -70 and -30 mV. This response was mimicked by 8-Br-cGMP These responses show that MCC can produce complex time and state-dependent activity during its modulation of the feeding neural circuit.

Keywords

cAMP cGMP guanylyl cyclase potassium channels -Aplysia 

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© Akadémiai Kiadó, Budapest 2004

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Department of Biological SciencesUniversity at Albany, State University of New YorkAlbanyUSA

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