Estradiol-Stimulated Nitric Oxide Release In Nervous Tissue, Vasculature, and Gonads of the Giant Cockroach Blaberus Craniifer
The vertebrate system of steroid hormones appears to have been conserved widely throughout the animal kingdom. The sex hormone estrogen, 17-β-estradiol (E2), long considered to be exclusively a vertebrate hormone, is found also in invertebrates related to reproductive and developmental processes such as spawning, vitellogenesis and molting. These processes are affected by estrogen induced changes at the genomic level and take place at a large time scale. The discovery of surface membrane receptors for E2 has opened new possibilities for the involvement of estrogen in biological functions other than reproductive. These processes take place within a few seconds to minutes and involve sudden cytosolic calcium transients, activation of adenylate cyclase or activation of phospholipase C (PLC). E2 can modulate the production of nitric oxide (NO) in endotheliar and other cells. A similar mechanism linking estrogen to cNOS catalized nitric oxide (NO) release is reported herein for the first time in several tissues of the giant cockroach Blaberus craniifer. This process has been identified in the brain, nerve cord, vasculature and ovaries. This effect is concentration dependent and is inhibited by tamoxifen an estrogen receptor blocker.
KeywordsEstradiol estrogen receptors nitric oxide invertebrate insect
Unable to display preview. Download preview PDF.
- 1.Bidmon, H. J., Stumpf, W. E. (1991) Uptake, distribution and binding of vertebrate and invertebrate steroid hormones and time-dependence of ponasterone A. binding in Calliphora vicina. Comparisons among cholesterol, corticosterone, Cortisol, dexamethasone, 5 alpha-dihydrotestosterone, 1,25-dihy-droxyvitamin D3, ecdysone, estradiol-17 beta, ponasterone A., progesterone, and testosterone. Histochemistry 96, 419–434.CrossRefGoogle Scholar
- 8.Keshan, B., Ray, A. K. (2001) The presence of estradiol-17beta and its specific binding sites in posterior silk gland of Bombyx mori. Gen. Comp. Endocrinol. 123, 23–30.Google Scholar
- 13.Levin, E. R. (2003) Bidirectional signaling between the estrogen receptor and the epidermal growth factor receptor. Mo/. Endocrinol. 17, 309–317.Google Scholar
- 14.Ogiso, M., Ohnishi, E. (1986) Does estradiol play a. role in ovarian maturation or embryonic development of the silkworm? Gen. Comp. Endocrinol. 61, 82–86.Google Scholar
- 17.Prevot, V., Croix, D., Rialas, C. M., Poulain, P., Fricchione, G. L., Stefano, G. B., Beauvillain, J. C. (1999) Estradiol coupling to endothelial nitric oxide stimulates gonadotropin-releasing hormone release from rat median eminence via a. membrane receptor. Endocrinology 140, 652–659.CrossRefGoogle Scholar
- 18.Stefano, G. B., Cadet, P., Breton, C., Goumon, Y., Prevot, V., Dessaint, J. P., Beauvillain, J. C., Roumier, A. S., Welters, I., Salzet, M. (2000) Estradiol-stimulated nitric oxide release in human granulocytes is dependent on intracellular calcium transients: evidence of a. cell surface estrogen receptor. Blood 95, 3951–3958.CrossRefGoogle Scholar
- 21.Stefano, G. B., Prevot, V., Beauvillain, J. C., Cadet, P., Fimiani, C., Welters, I., Fricchione, G. L., Breton, C., Lassalle, P., Salzet, M., Bilfmger, T. V. (2000) Cell-surface estrogen receptors mediate calcium-dependent nitric oxide release in human endothelia. Circulation 101, 1594–1597.CrossRefGoogle Scholar
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.