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Acta Biologica Hungarica

, Volume 54, Issue 3–4, pp 285–298 | Cite as

A Comparative Methodical Study of the Faecal Steroid Analysis on Birds: Looking for A Valid Method of Testosterone Determination

  • K. KelemenEmail author
  • P. Péczely
  • Zs. Szőke
  • V. Ladjánszky
Article

Abstract

In a comparative study, a relatively simple and high sensitivity method was developed for analysis of testosterone-equivalent(s) in the faeces of different bird species. To determine the recovery of extractions and purifications, tritium-labelled testosterone was added to the wet samples. Then the samples were treated with sodium dodecil sulphate (SDS), an emulsificator to “open-up” the complex, lipid-coated particles of faecal samples. This emulsification resulted in the decrease of the quantity of interfering substances after diethyl-ether extraction and the linearity of the measured testosterone equivalents from aliquots in the range of 2 and 10 mg of faeces. In the RIA, we applied a group specific polyclonal testosterone antibody which cross-reacted with reduced metabolites and at a certain level with sulphate conjugates as well. The use of Helix enzymes did not modified significantly the results of the analysis relating to a low level of conjugated androgens in the faecal extracts. The biological validity of the method was tested on domestic cockerels, where between the plasma and faecal testosterone values a four hours phase shift was observed, with a correlation of 0.6355. This method is suitable for “non invasive”, behavioural-ethological studies.

Keywords

Steroids testosterone faeces birds methods biochemistry 

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Notes

Acknowledgements

This work was supported by a grant from OTKA, No. T030769. We are grateful to Mrs Katalin Bara for her excellent technical assistance and to Mr. Szilamér Ferenczi for his consultative assistance.

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© Akadémiai Kiadó, Budapest 2003

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • K. Kelemen
    • 1
    Email author
  • P. Péczely
    • 1
  • Zs. Szőke
    • 1
  • V. Ladjánszky
    • 1
  1. 1.Department of Reproductive BiologySzt. István UniversityGödöllőHungary

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