A Comparative Methodical Study of the Faecal Steroid Analysis on Birds: Looking for A Valid Method of Testosterone Determination

Abstract

In a comparative study, a relatively simple and high sensitivity method was developed for analysis of testosterone-equivalent(s) in the faeces of different bird species. To determine the recovery of extractions and purifications, tritium-labelled testosterone was added to the wet samples. Then the samples were treated with sodium dodecil sulphate (SDS), an emulsificator to “open-up” the complex, lipid-coated particles of faecal samples. This emulsification resulted in the decrease of the quantity of interfering substances after diethyl-ether extraction and the linearity of the measured testosterone equivalents from aliquots in the range of 2 and 10 mg of faeces. In the RIA, we applied a group specific polyclonal testosterone antibody which cross-reacted with reduced metabolites and at a certain level with sulphate conjugates as well. The use of Helix enzymes did not modified significantly the results of the analysis relating to a low level of conjugated androgens in the faecal extracts. The biological validity of the method was tested on domestic cockerels, where between the plasma and faecal testosterone values a four hours phase shift was observed, with a correlation of 0.6355. This method is suitable for “non invasive”, behavioural-ethological studies.

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Acknowledgements

This work was supported by a grant from OTKA, No. T030769. We are grateful to Mrs Katalin Bara for her excellent technical assistance and to Mr. Szilamér Ferenczi for his consultative assistance.

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Correspondence to K. Kelemen.

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Kelemen, K., Péczely, P., Szőke, Z. et al. A Comparative Methodical Study of the Faecal Steroid Analysis on Birds: Looking for A Valid Method of Testosterone Determination. BIOLOGIA FUTURA 54, 285–298 (2003). https://doi.org/10.1556/ABiol.54.2003.3-4.7

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Keywords

  • Steroids
  • testosterone
  • faeces
  • birds
  • methods
  • biochemistry