Acta Biologica Hungarica

, Volume 54, Issue 3–4, pp 275–283 | Cite as

Immunohistochemical Analysis of Substance P Containing Nerve Fibres and Their Contacts with Mast Cells in the Diabetic Rat’s Tongue

  • Bayarchimeg Batbayar
  • J. Somogyi
  • T. Zelles
  • Erzsébet FehérEmail author


Sensory neuropathy is common symptom of the diabetes mellitus and the prevalence of oral lesions is higher in diabetic patients. The distribution of substance P was studied immunohistochemically in streptozotocin induced diabetic rat’s tongue. The morphological association of sensory nerves (substance P immunoreactive) with mast cells (nerve fibre-mast cell contact) was monitored. The substance P nerve fibre-mast cell contacts were very scanty in control tongue. The number of substance P nerve terminals and mast cells was significantly increased (p < 0.05) in diabetes mellitus after 4 weeks of the treatment compared with the control tongue. The number of mast cell-nerve contacts was even more significantly increased (p < 0.001) in diabetes. The distance between nerve fibres and mast cells was about 1 mm and very often less than 200 nm. In some instances, the mast cells were degranulated in the vicinity to nerve fibres. Increased number of mast cell-nerve contacts in neurogenic inflammation might cause vasoconstriction and lesions of the oral mucosa, so some disorders such lichen planus, leukoplakia and cancer might frequently develop in diabetes mellitus.


Substance P inflamed tongue diabetic rat streptozotocin immunohistochemistry 


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© Akadémiai Kiadó, Budapest 2003

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Bayarchimeg Batbayar
    • 1
  • J. Somogyi
    • 2
  • T. Zelles
    • 3
  • Erzsébet Fehér
    • 1
    Email author
  1. 1.Laboratory of Oral Morphology, Department of AnatomyHistology and EmbryologyBudapestHungary
  2. 2.Department of Medical ChemistryMolecular Biology and PathobiochemistryBudapestHungary
  3. 3.Department of Oral BiologySemmelweis UniversityBudapestHungary

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