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Acta Biologica Hungarica

, Volume 54, Issue 3–4, pp 275–283 | Cite as

Immunohistochemical Analysis of Substance P Containing Nerve Fibres and Their Contacts with Mast Cells in the Diabetic Rat’s Tongue

  • Bayarchimeg Batbayar
  • J. Somogyi
  • T. Zelles
  • Erzsébet FehérEmail author
Article

Abstract

Sensory neuropathy is common symptom of the diabetes mellitus and the prevalence of oral lesions is higher in diabetic patients. The distribution of substance P was studied immunohistochemically in streptozotocin induced diabetic rat’s tongue. The morphological association of sensory nerves (substance P immunoreactive) with mast cells (nerve fibre-mast cell contact) was monitored. The substance P nerve fibre-mast cell contacts were very scanty in control tongue. The number of substance P nerve terminals and mast cells was significantly increased (p < 0.05) in diabetes mellitus after 4 weeks of the treatment compared with the control tongue. The number of mast cell-nerve contacts was even more significantly increased (p < 0.001) in diabetes. The distance between nerve fibres and mast cells was about 1 mm and very often less than 200 nm. In some instances, the mast cells were degranulated in the vicinity to nerve fibres. Increased number of mast cell-nerve contacts in neurogenic inflammation might cause vasoconstriction and lesions of the oral mucosa, so some disorders such lichen planus, leukoplakia and cancer might frequently develop in diabetes mellitus.

Keywords

Substance P inflamed tongue diabetic rat streptozotocin immunohistochemistry 

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References

  1. 1.
    Albrecht, M., Banoczy, J., Dinya, T., Tamas, G. Jr. (1992) Occurrence of oral leukoplakia and lichen planus in diabetes mellitus. J. Oral Path. Med. 21, 364–366.CrossRefGoogle Scholar
  2. 2.
    Arzubiaga, C., Morrow, J., Roberts, J., Biaggioni, I. (1991) Neuropeptide Y., a. putative cotransmitter in noradrenergic neurones, induces mast cell degranulation but not prostaglandin D2 release. J. Allergy Clin. Immunol. 87, 88–93.CrossRefGoogle Scholar
  3. 3.
    Bos, J. D., Hulsebosch, H. J., Krieg, S. R., Bakker, R. M., Cormane, R. H. (1983) Immunocompetent cells in psoriasis. Arch. Dermatol. Res. 275, 181–189.CrossRefGoogle Scholar
  4. 4.
    Botchkarev, V. A., Eichmuller, S., Peters, E. M., Pietsch, P., Johansson, O., Maurer, M., Pauss, R. (1997) A. simple immunofluorescence technique for simultaneous visualisation of mast cells and nerve fibres reveals selectivity and hair cycle-dependent changes in mast cell-nerve fibre contacts in murine skin. Arch. Dermatol. Res. 289, 292–302.CrossRefGoogle Scholar
  5. 5.
    Boulton, A. J. M., Ward, J. D. (1986) Diabetic neuropathies and pain. Clin. Endocrinol. Metab. 15, 917–931.CrossRefGoogle Scholar
  6. 6.
    Britland, S. T., Young, R. J., Sharma, A. K., Clarke, B. F. (1992) Acute and remitting painful diabetic polyneuropathy: a. comparison of peripheral nerve fibre pathology. Pain 48, 361–370.CrossRefGoogle Scholar
  7. 7.
    Dimitriadou, V., Rouleau, A., Trung-Tuong, M. D., Newlands, G. J., Miller, H. R., Luffau, G., Schwartz I. C., Garbarg, M. (1997) Functional relationships between sensory nerve fibres and mast cells of dura mater in normal and inflammatory conditions. Neuroscience 77, 829–839.CrossRefGoogle Scholar
  8. 8.
    Dvorak, A. M., McLeod, R. S., Onderdonk, A. B., Monahan-Earley, R. A., Cullen, J. B., Antonioli, D. A., Morgan, E., Blair, J. E., Estrella, P., Cisneros, R. L. (1992) Human gut mucosal mast cells ultrastructural observations and anatomical variation in mast cell nerve association in vivo. Int. Arch. Allergy. Immunol. 98, 158–168.CrossRefGoogle Scholar
  9. 9.
    Dyck, P. J., Lambert, E. H., O’Brien, R. C. (1976) Pain in peripheral neuropathy related to rate and kind of fibre degeneration. Neurology 26, 466–171.CrossRefGoogle Scholar
  10. 10.
    Ebertz, J. M., Hirshman, C. A., Kettelkamp, N. S., Uno, H., Hanifin, J. M. (1987) Substance P-induced histamine release in human cutaneous mast cells. J. Invest. Dermatol. 88, 682–685.CrossRefGoogle Scholar
  11. 11.
    Fehér, E., Altdorfer, K., Bagaméri, G., Fehér, J. (2001) Neuroimmune interactions in experimental colitis. Neuroimmunomodulation 9, 247–256.CrossRefGoogle Scholar
  12. 12.
    Foster, C. A., Mandak, B., Kromer, E., Rot, A. (1991) The neuropeptide Substance P. is chemotactive for human T. cells. Clin. Res. 39, 535A.Google Scholar
  13. 13.
    Fowler, J. J., Ochoa, J. (1975) Unmyelinated fibres in normal and compressed peripheral nerves of the baboon: a. quantitative electron microscopic study. Neuropath. Appl. Neurobiol. 247–265.Google Scholar
  14. 14.
    Geppetti, R., Holzer, P. (1996) Neurogenic inflammation. Boca Raton, FL: CRC PressGoogle Scholar
  15. 15.
    Hartung, H. P., Toyka, K. V. (1989) Substance P., the immune system and inflammation. Int. Rev. Immunol. 4, 229–249.CrossRefGoogle Scholar
  16. 16.
    Holzer, P. (1998) Neurogenic vasodilatation and plasma leakage in the skin. Gen. Pharmacol. 30, 5–11.CrossRefGoogle Scholar
  17. 17.
    Kulkari-Narla, A., Beitz, A. J., Brown, D. R. (1999) Catecholaminergic, cholinergic and peptidergic innervation of gut-associated lymphatic tissue in porcine jejunum and ileum. Cell. Tissue Res. 298, 275–286.CrossRefGoogle Scholar
  18. 18.
    Llewelyn, J. G., Thomas, P. K., Fonseca, V., King, R. H. M., Dandona, R. (1986) Acute painful dia-betic neuropathy precipitated by strict glycaemic control. Acta Neuropathol. (Berl.) 72, 157–163.CrossRefGoogle Scholar
  19. 19.
    Lozada-Nur, F., Miranda, C. (1997) Oral lichen planus: epidemiology, clinical characteristics and associated diseases. Seminars in Cutaneous Medicine and Surgery 16, 273–277.CrossRefGoogle Scholar
  20. 20.
    Makkos, G., Szűcs, J., Kerényi, Z. et al. (1987) Az első hazai diabeteses tömegszűrés az EVSZ 1980-as új diagnosztikus kritériumainak alkalmazasaval. Népegészségügy 68, 138–141.Google Scholar
  21. 21.
    Matis, W. L., Lavker, R. M., Murphy, G. F. (1990) Substance P. induces the expression of an endothe-lial-leukocyte adhesion molecule b. microvascular endothelium. J. Invest. Dermatol. 94, 492–195.CrossRefGoogle Scholar
  22. 22.
    Matsunaga, Y., Kawasaki, H., Terada, T. (1999) Stromal mast cells and nerve fibres in various chron-ic liver disease; relevance to hepatic fibrosis. Am. J. Gastroenterol. 94, 1923–1932.CrossRefGoogle Scholar
  23. 23.
    Nagato, T., Ren, X., Ton, H., Tandler, B. (1997) Ultrastructure of Weber’s salivary glands of the root of the tongue in the rat. The Anatomical Record 249, 435–140.CrossRefGoogle Scholar
  24. 24.
    Naukkarinen, A., Harvima, I., Paukkonen, K., Aalto, M. L., Horsmanheimo, M. (1993) Immuno-histochemical analysis of sensory nerves and neuropeptides, and their contacts with mast cells in developing and mature psoriatic lesions. Arch. Dermatol. Res. 285, 341–346.CrossRefGoogle Scholar
  25. 25.
    Ottaway, C. A., Stanisz, A. M. (1995) Neural-immune interactions in the intestine: Implications of inflammatory bowel disease: In: Karger J. B., Shorter R. G. (eds) Inflammatory Bowel Disease. Baltimore: Williams and Wilkins, 281–300.Google Scholar
  26. 26.
    Ruocco, I., Cuello, A. C., Shigemoto, I., Ribeiro-da-Silva, A. (2001) Light and electronmicroscopic study of the distribution of substance P-immunoreactive fibers and neurokinin-1 receptors in the skin of the rat lower lip. J. Comp. Neurol. 432, 466–180.CrossRefGoogle Scholar
  27. 27.
    Stead, R. H., Perdue, M. H., Blennerhassett, M. G., Kakuta, Y., Sestini, P., Bienenstock, J. (1990) The innervation of mast cells. In: Freier, S. (ed.) The neuroendocrine-immune network. Boca Raton, FL: CRC Press, 19–37.Google Scholar
  28. 28.
    Tandan, R., Lewis, G. A., Krusinski, P. B., Badger, G. B., Fries, T. J. (1992) Topical capsaicin in painful diabetic neuropathy (controlled study with long-term follow up). Diabetes Care 15, 8–14.CrossRefGoogle Scholar
  29. 29.
    Thomas, R. K. (1994) Painful diabetic neuropathy: mechanisms and treatment. Diabet. Nutr. Metab. 7, 359–368.Google Scholar
  30. 30.
    Újpál, M., Matos, O., Bíbók, G., Szabó, G. (2002) A. diabetes mellitus előfordulása malignus szajüre-gi daganatos betegekben. Orvosi Hetilap 143, 2731–2733.PubMedGoogle Scholar
  31. 31.
    Williams, R. M., Berthoud, H. R., Stead, R. H. (1997) Vagal afferent nerve fibres contact mast cells in rat small intestinal mucosa. Neuroimmun. 4, 266–270.CrossRefGoogle Scholar
  32. 32.
    Williams, R. M., Bienenstock, J., Stead, R. H. (1995) Mast cells: the neuroimmune connection. In: Marone, G. (ed.) Human Basophils and Mast Cells: Biological Aspects. vol. 61. Basel: Karger, 208–235.Google Scholar
  33. 33.
    Yohenara, N., Yoshimura, M. (2001) Influence of painful chronic neuropathy on neurogenic inflammation. Pain 92, 259–265.CrossRefGoogle Scholar
  34. 34.
    Zhang, W. Y., Po, A. L. W. (1994) The effectiveness of topically applied capsaicin: a. meta-analysis. Eur. J. Clin. Pharmacol. 46, 517–522.CrossRefGoogle Scholar
  35. 35.
    Zhao, Z. Z., Savage, N. W., Pujic, Z., Walsh, L. J. (1997) Immunohistochemical localisation of mast cells and mast cell-nerve interactions in oral lichen planus. Oral Dis. 3: 71–76.Google Scholar

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© Akadémiai Kiadó, Budapest 2003

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Bayarchimeg Batbayar
    • 1
  • J. Somogyi
    • 2
  • T. Zelles
    • 3
  • Erzsébet Fehér
    • 1
    Email author
  1. 1.Laboratory of Oral Morphology, Department of AnatomyHistology and EmbryologyBudapestHungary
  2. 2.Department of Medical ChemistryMolecular Biology and PathobiochemistryBudapestHungary
  3. 3.Department of Oral BiologySemmelweis UniversityBudapestHungary

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